[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Number 2 (Bimonthly 2017) ::
Feyz 2017, 21(2): 126-133 Back to browse issues page
Cytotoxic and antioxidant effect of chrysin on neonate mouse spermatogenic stem cells
Mehran Pordel, Javad Baharara , Elaheh Amini
Professor Research Center of Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran. , baharara@yahoo.com
Abstract:   (315 Views)

Background: So far, many plants have been used for the treatment of infertility. Several studies have revealed that chrysin (as an active metabolite) improves animals' reproduction. Therefore, the objective of this study was to evaluate the effect of chrysin on Balb/C mice spermatogenic stem cells.

 Materials and Methods: In this in vitro experimental study Balb/C neonate spermatogonia stem cells cultured in DMEM-F12 medium were treated with various concentrations of chrysin (2.5, 5, 10, 20, 40 µg/ml) for 6 and 12 days. Then the cytotoxicity was assessed using MTT, Akredin orange/Propodium Idid, DAPI and antioxidant concentration DCF-DA tests.

Results: Chrysin showed no remarkable cytoxicity in concentrations less than 5 µg/ml. While, after 6 days the viability of cells treated with chrysin 10, 20 and 40 µg/ml  was decreased to  30, 45 and 56 %  (P<0.05 and P<0.001, respectiely); after 12 days the viability of cells was decreased to 44, 56 and 65 % (P<0.05, P<0.01 and P<0.001, respectiely). DCF-DA results revealed a 80 % antioxidant capacity of chrysin in 5 and 2.5µg/ml concentrations.

Conclusion: Lower concentrations of chrysin has protective effect on Balb/C mice spermatogenic through improving cell viability, decreasing cells apoptosis and inhibiting free radicals.

Keywords: Chrysin, Antioxidant, Cytotoxicity, Infertility, Spermatogenic stem cells
Full-Text [PDF 464 kb]   (156 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/05/24 | Accepted: 2017/05/24 | Published: 2017/05/24
References
1. Venkatesh T, Suresh PS, Tsutsumi R. New insights into the genetic basis of infertility. Appl Clin Genet 2014; 7: 235-43.
2. Palmer NO, Bakos HW, Fullston T, Lane M. Impact of obesity on male fertility, sperm function and molecular composition. Spermatogenesis 2012; 2(4): 253-63.
3. Hermann BP, Sukhwani M, Winkler F, Pascarella JN, Peters KA, Sheng Y, et al. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell 2012; 11(5): 715-726.
4. Vasei N, Baharara J, Zafar Balanezhad S, Amini E. An investigation of the protective impact exerted by aqua extract of Persian Gulf sea cucumber (Holothuria arenicola Semper, 1868) against damages induced by electromagnetic field on male gonads of Balb/C mice. – Nova Biol. Reperta 2015; 2: 216-26.
5. Walker WH. Testosterone signaling and the regulation of spermatogenesis. Spermatogenesis 2011; 1(2): 116-20.
6. Dym M, Kokkinaki M, He Z. Spermatogonial stemcells: mouse and human comparisons. Birth Defects Res C Embryo Today 2009; 87(1): 27-43.
7. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of Oxidative Stress on Male Reproduction. World J Mens Health 2014; 32(1): 1-17.
8. Safarinejad MR. Infertility among couples in a population-based study in Iran: prevalence and associated risk factors. Int J Androl 2008; 31(3): 303-314.
9. Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach. BJU Int 2005; 95(4): 503-7.
10. Kumar R, Venkatesh S, Kumar M, Tanwar M, Shasmsi MB, Kumar R, et al. Oxidative stress and sperm mitochondrial DNA mutation iidiopathic oligoasthenozoospermic men. Indian J Biochem Biophys 2009; 46(2): 172-7.
11. Walczak–Jedrzejowska R, Wolski JK, Slowikowska–Hilczer J. The role of oxidative stress and antioxidants in male fertility. Cent European J Urol 2013; 66(1): 60-67.
12. Safarnavadeh T, Rastegarpanah M. Antioxidants and infertility treatment, the role of Satureja Khuzestanica: A mini-systematic review. Iran J Reprod Med 2011; 9(2): 61-70.
13. Seandel M, James D, Shmelkov SV, Falciatori I, Kim J, Chavala S, et al. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature 2007; 449(7160): 346-50.
14. Amini Sarteshnizi N, Teimori H, Zahri S, Mobini Dehkordi M, et al. Effect of Chrysin on AGS human gastric cancer cell line. J Gorgan Uni Med Sci 2015; 16(4): 63-8. [in Persian]
15. Ciftci O, Ozdemir I, Aydin M, Beytur A. Beneficial effects of chrysin on the reproductive system of adult male rats. Andrologia 2012; 44(3): 181-6.
16. Mehri S, Veis Karami H, Vahdati Hassani F, Hosseinzadeh H. Chrysin Reduced Acrylamide-Induced Neurotoxicity in Both in vitro and in vivo Assessments. Iran Biomed J 2014; 18(2): 101-6.
17. Huang YH, Chin CC, Ho HN, Chou CK, Shen CN, Kuo HC, et al. Pluripotency of mouse spermatogonial stem cells maintained by IGF-1- dependent pathway. FASEB J 2009; 23(7): 2076-87.
18. Amini E, Baharara J, Nikdel N, Salek Abdollahi F. Cytotoxic and Pro-Apoptotic Effects of Honey Bee Venom and Chrysin on Human Ovarian Cancer Cells. Asia Pacific J Med Toxicol 2015; 4(2): 68-73.
19. Hara K, Nakagawa T, Enomoto H, Suzuki M, Yamamoto M, Simons BD, et al. Mouse spermatogenic stem cells continually interconvert between equipotent singly isolated and syncytial states. Cell Stem Cell 2014; 14(5): 658-72.
20. Bjelakovic G, Nikolova D, Gluud C. Antioxidant supplements and mortality. Curr Opin Clin Nutr Metab Care 2014; 17(1): 40-4.
21. Gholami M, Saki G, Hemadi M, Khodadadi A, Mohammadi-asl J. Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice. Iran J Basic Med Sci 2014; 17(2): 93-9.
22. Ciftci O, Ozdemir I, Aydin M, Beytur A, Beneficial effects of chrysin on thereproductive systemof adult male rats. Andrologia 2012; 44(3): 181-6.
23. Alkan I, Simsek F, Haklar G, Kervancioglu E, Ozveri H, Yalcin S, et al. Reactive oxygen species production by the spermatozoa of patients with idiopathic infertility: relationship to seminal plasma antioxidants. J Urol 1997; 157(1): 140-3.
24. Miller JK, Brzezinska-Slebodzinska E, Madsen FC. Oxidative stress, antioxidants, and animal function. J Dairy Sci 1993; 76(9): 2812-23.
25. Bekdeşer B, Durusoy N, Özyürek M, Güçlü K, Apak R. Optimization of microwave-assisted extraction of polyphenols from herbal teas and evaluation of their in vitro hypochlorous acid scavenging activity. J Agric Food Chem 2014; 62(46): 11109–15.
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pordel M, Baharara J, Amini E. Cytotoxic and antioxidant effect of chrysin on neonate mouse spermatogenic stem cells. Feyz. 2017; 21 (2) :126-133
URL: http://feyz.kaums.ac.ir/article-1-3346-en.html
Volume 21, Number 2 (Bimonthly 2017) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.053 seconds with 813 queries by yektaweb 3461