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:: Volume 21, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 21(6): 569-576 Back to browse issues page
Association between miR Let-7g gene expression and the risk of cervical cancer in human papillomavirus-infected patients
Fatemeh Barazandeh , Khadijeh Onsory *, Anahita Mohseni-Meybodi
Associated Professor Department of Biology, Faculty of Science, Parand Branch, Islamic Azad University, Parand, I. R. Iran. , onsory@gmail.com
Abstract:   (261 Views)
Background: The miR Let-7g expression has been investigated in several cancers but no report is available on cervical cancer patients. The aim of this study was to evaluate the association between miR Let-7g expression and the risk of cervical cancer in human papillomavirus (HPV)-infected patients.
Materials and Methods: In this case-control study, 20 paraffin-embedded tissue samples were collected from HPV-infected cervical cancer patients, 20 samples from patients with no infection and 20 healthy women referred to the Mirzakochak Khan Jangali Hospital in Tehran in 2015. Then, the miR Let-7g gene expression was measured by the real-time polymerase chain reaction method.
Results: The results showed that miR Let-7g expression was decreased in HPV-infected and non-infected cervical cancer patients compared to the control group (P=0.0001and P=0.03, respectively). There was a significant decrease in miR Let-7g expression in HPV-infected patients compared to non-infected ones (P=0.0001). Also, a positive correlation was observed between decreased miR Let-7g expression and the stage of the disease, but no significant correlation was found between age of the patients and the level of gene expression in the studied population.
Conclusion: According to the results of the present study, the miR Let-7g expression in cervical cancer patients is significantly decreased compared to the control group. Therefore, evaluation of the microRNA expression can be used as a valuable prognostic marker for early diagnosis of cervical cancer.
Keywords: Cervical cancer, Human papillomavirus, MicroRNA Let7g gene expression, Real-time PCR
Full-Text [PDF 513 kb]   (56 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/02/19 | Accepted: 2017/12/11 | Published: 2017/12/24
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Barazandeh F, Onsory K, Mohseni-Meybodi A. Association between miR Let-7g gene expression and the risk of cervical cancer in human papillomavirus-infected patients. Feyz. 2017; 21 (6) :569-576
URL: http://feyz.kaums.ac.ir/article-1-3268-en.html

Volume 21, Issue 6 (Bimonthly 2017) Back to browse issues page
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