[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 21(6): 569-576 Back to browse issues page
Association between miR Let-7g gene expression and the risk of cervical cancer in human papillomavirus-infected patients
Fatemeh Barazandeh, Khadijeh Onsory *, Anahita Mohseni-Meybodi
Department of Biology, Faculty of Science, Parand Branch, Islamic Azad University, Parand, I. R. Iran. , onsory@gmail.com
Abstract:   (1416 Views)
Background: The miR Let-7g expression has been investigated in several cancers but no report is available on cervical cancer patients. The aim of this study was to evaluate the association between miR Let-7g expression and the risk of cervical cancer in human papillomavirus (HPV)-infected patients.
Materials and Methods: In this case-control study, 20 paraffin-embedded tissue samples were collected from HPV-infected cervical cancer patients, 20 samples from patients with no infection and 20 healthy women referred to the Mirzakochak Khan Jangali Hospital in Tehran in 2015. Then, the miR Let-7g gene expression was measured by the real-time polymerase chain reaction method.
Results: The results showed that miR Let-7g expression was decreased in HPV-infected and non-infected cervical cancer patients compared to the control group (P=0.0001and P=0.03, respectively). There was a significant decrease in miR Let-7g expression in HPV-infected patients compared to non-infected ones (P=0.0001). Also, a positive correlation was observed between decreased miR Let-7g expression and the stage of the disease, but no significant correlation was found between age of the patients and the level of gene expression in the studied population.
Conclusion: According to the results of the present study, the miR Let-7g expression in cervical cancer patients is significantly decreased compared to the control group. Therefore, evaluation of the microRNA expression can be used as a valuable prognostic marker for early diagnosis of cervical cancer.
Keywords: Cervical cancer, Human papillomavirus, MicroRNA Let7g gene expression, Real-time PCR
Full-Text [PDF 513 kb]   (719 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/02/19 | Accepted: 2017/12/11 | Published: 2017/12/24
1. Ferlay J, Steliarova-Foucher E, Lortet-Tieulent J, Rosso S, Coebergh JW, Comber H, et al. Cancer incidence and mortality patterns in Europe: estimates for 40 countries in 2012. Eur J Cancer 2013; 49(6): 1374-403.
2. Kitchener HC, Almonte M, Thomson C, Wheeler P, Sargent A, Stoykova B, et al. HPV testing in combination with liquidbased cytology in primary cervical screening (ARTISTIC): a randomized controlled trial. Lancet Oncol 2009; 10(7): 627-82.
3. Bernard HU, Burk RD, Chen Z, van Doorslaer K, Zur Hausen H, de Villiers EM. Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments. Virology 2010: 401(1): 70-9.
4. Schiffman M, Wentzensen N. A suggested approach to simplify and improve cervical screening in the united states. J Low Genit Tract Dis 2016; 20(1): 1-7.
5. Adebamowo SN, Olawande O, Famooto A, Dareng EO, Offiong R, Adebamowo CA Persistent Low-Risk and High-Risk Human Papillomavirus Infections of the Uterine Cervix in HIV-Negative and HIV-Positive Women. Front Public Health 2017; 5: 178.
6. Miska EA. How microRNAs control cell division, differentiation and death. Curr Opin Genet Dev 2005; 5(15): 563-8.
7. Boyerinas B, Park SM, Hau A, Murmann AE, Peter ME. The role of let-7 in cell differentiation and cancer. Endocr Related Cancer 2010; 17(1): F19-36.
8. Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, et al. Frequent deletions and down-regulation of microRNA genes miR16 at 13q14 in chronic lymphocytic leukemia. Pro Natl Acad Sci USA 2002; 99 (24): 15524-29.
9. Johnson CD, Esquel-kerscher A, Stefani G, Byrom M, Kelnar K, Ovcharenko D, et al. The let-7 microRNA represses cell proliferation pathways in human cells. Cancer Res 2007; 67(16): 7713–22.
10. Wu L, Wang Q, Yao J, Jiang H, Xiao C, Wu F, et al. MicroRNA Let-7g and Let-7i inhibit hepatoma cell growth concurrently via down regulation of the anti-apoptotic protein B-Cell lymphoma- extra large. Oncol Lett 2015; 9(1): 213-18.
11. Barh D, Malhotra R, Ravi B, Sindhurani P. MicroRNA Let-7: an emerging next- generation cancer therapeutic. Current Oncol 2010; 17(1): 70-80.
12. Park J, Sun D, Genest DR, Trivijitsilp P, Suh I, Crum CP. Coexistence of Low and High Grade Squamous Intraepithelial Lesions of the Cervix: Morphologic Progression or Multiple Papillomaviruses? Gynecol Oncol 1998; 70(3): 386-91.
13. Buechner J, Tomte E, Haug BH, Henriksen JR, Lokke C, Flægstad T, et al. Tumour-suppressor microRNAs let-7 and Mir-101 Target the protooncogene MYCN and inhibit cell proliferation in MYCN-amplified neuroblastoma. Br J Cancer 2011; 105(2): 296-303.
14. Peter ME. Let-7 and miR-200 microRNAs: guardians against pluripotency and cancer progression. Cell Cycle 2009; 8(6): 843-52.
15. Siomi H, Siomi MC. Posttranscriptional regulation of microRNA biogenesis in animals. Mol Cell 2010; 38(3): 323-32.
16. Vousden KH. Interactions between papillomavirus proteins and tumor suppressor gene products. Adv Cancer Res 1994; 64: 1-24.
17. Hooi DJ, Lissenberg-Witte BI, de Koning MNC, Pinedo HM, Kenter GG, Meijer CJLM, et al. High prevalence of high-risk HPV genotypes other than 16 and 18 in cervical cancers of Curaçao: implications for choice of prophylactic HPV vaccine. Sex Transm Infect 2017. 11. pii: sextrans-2017-053109. [Epub ahead of print]
18. Tewari D, Novak-Weekley S, Hong C, Aslam S, Behrens CM. Performance of the cobas HPV Test for the Triage of Atypical Squamous Cells of Undetermined Significance Cytology in Cervical Specimens Collected in SurePath. Am J Clin Pathol 2017; 148(5): 450-7.
19. [19] Lee YS, Dutta A. The tumor suppressor microRNA let-7 represses the HMGA2 oncogene. Genes Dev 2007; 21 (9): 1025–30.
20. Qian P, Zuo Z, Wu Z, Meng X, Li G, Wu Z, et al. Pivatal role of reduced Let-7g expression in breast cancer invasion and metastasis. Cancer Res 2011; 71(20): 6463-74.
21. Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, et al. Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res 2004; 64 (11): 3753–56.
22. Mayer C, Hemann MT, Bartel DP. Disrupting the pairing between Let-7 and HMGA2 enhances oncogenic transformation. Science 2007; 315(5818): 1576-79.
23. Guled M, Lahti L, Lindholm PM, Salmenkivi K, Bagwan I, Nicholson AG, et al. CDKN2A, NF2 and JUN are dysregulated among other genes by miRNAs in malignant mesothelioma-A miRNA microarray analysis. Genes Chromosome Cancer 2009; 48(7): 615-23.
24. Ross SA, Davis CD. MicroRNA, nutrition and cancer prevention. Adv Nutr 2011; 2(6):472-85.
25. Esquela- Kerscher A, Trang P, Wiggins JF, Patrawala L, Cheng A, Ford L, et al. The Let-7 microRNA reduces tumor growth in mous models of lung cancer. Cell Cycle 2008; 7(6): 759-64.
26. Oliveras-Ferraros C, Cufi S, Vazquez-Martin A, Torres-Carcia VZ, Del Barco S, Martin-Castillo B, et al. Micro(mi) RNA prperssion profile of breast cancer epithelial cells treated with the anti- diabetic drug metformin: induction of the tumor suppressor miRNA Let-7a and suppression of the TGFB- induced oncomir miRNA- 181a. Cell Cycle 2011; 10(7): 1144-51.
27. Simon P, Fehrenbach E, Niess AM. Regulation of immediate early gene expression by exercise: short cuts for the adaptation of immune function. Exerc Immunol Rev 2006; 12: 112-31.
28. Fendler A, Stephan C, Yousef GM, Jung K. MicroRNAs as regulators of signal transduction in urological tumors. Clin Chem 2011; 57(7): 954-68.
29. Chen KJ, Hou Y, Wang K, Li J, Xia Y, Yang XY, et al. Re-expression of Let-7g MicroRNA Inhabits the Proliferation and Migration via K-Ras/ HMGA2/Snail AXIS IN Hepato cellular Carcinoma. Biomed Res Int 2014; 2014: 742417
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Barazandeh F, Onsory K, Mohseni-Meybodi A. Association between miR Let-7g gene expression and the risk of cervical cancer in human papillomavirus-infected patients. Feyz. 2017; 21 (6) :569-576
URL: http://feyz.kaums.ac.ir/article-1-3268-en.html

Volume 21, Issue 6 (Bimonthly 2017) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 4110