:: Volume 20, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 20(6): 525-531 Back to browse issues page
Phenotypic evaluation of biofilm producing ability in Methicillin resistant Staphylococcus aureus
Naghmeh Moori-Bakhtiari *, Maryam Moslemi
Department of Microbiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, I. R. Iran. , n.moori@scu.ac.ir
Abstract:   (2313 Views)

Background: Like genomic changes, the ability for biofilm production is considered as one of the antibiotic resistant factors in bacteria which can cause recurrent infections.  The infection resulted from Methicillin-resistant staphylococcus aureus is the most common form of such complications manifested as recurrent infections. The aim of this study was to investigate biofilm production ability among isolated Methicillin-resistant Staphylococcus aureus (MRSA) in patients with different types of clinical infection.

Material and Methods: Fifty Staphylococcus aureus strains were isolated from different specimens and identified by biochemical and species-specific PCR tests. Methicillin- resistance specificity of isolates was confirmed by disk diffusion method and mecA gene presence; the biofilm- forming ability was evaluated by crystal violet microtiter plate assay and Congo red agar (CRA).

Results: Using turbidimetry with no acetic acid, the ability for biofim production was seen at 550 and 492 nm in 34 (68%) and 28 isolates (56%), respectively. In both methods, the most of isolates were weak biofilm producers. In CRA, 94% of isolates were biofilm producers which most (72.3%) of them were moderate producers.

Conclusions: While with the consideration of three studied methods high percentages of isolates were biofilm producers and despite the significant correlation seen between their results, there was a higher correlation coefficient between the results obtained from crystalviolet-treated microtiter plates with two reading methods.

Keywords: Methicillin-resistant staphylococcus aureus, Biofilm, Crystal violet microtiter plate, Congo red agar medium
Full-Text [PDF 233 kb]   (528 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/01/2 | Accepted: 2017/01/2 | Published: 2017/01/2
1. Lora-Tamayo J, Murillo O, Iribarren JA, Soriano A, Sánchez-Somolinos M, Baraia-Etxaburu JM, et al. A large multicenter study of methicillin-susceptible and methicillin-resistant Staphylococcus aureus prosthetic joint infections managed with implant retention. Clin Infect Dis 2013; 56(2): 182–94.
2. Salgado CD, Dash S, Cantey JR, Marculescu CE. Higher risk of failure of methicillin-resistant Staphylococcus aureus prosthetic joint infections. Clin Orthop Relat Res 2007; 461: 48–53.
3. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science 1999; 284(5418): 1318–22.
4. Swan A, Amer H, Dieppe P. The value of synovial fluid assays in the diagnosis of joint disease: a literature survey. Ann Rheum Dis 2002; 61(6): 493–8.
5. Simon GL, Miller HG, Borenstein DG. Synovial fluid inhibits killing of Staphylococcus aureus by neutrophils. Infect Immun 1983; 40(30): 1004–10.
6. Dastgheyb S, Parvizi J, Shapiro IM, Hickok NJ, Otto1 M. Effect of Biofilms on Recalcitrance of Staphylococcal Joint Infection to Antibiotic Treatment. J Infectious Dis 2015; 211(4): 641–50
7. Post JC, Stoodley P, Hall-Stoodley L, Ehrlich GD. The role of biofilms in otolaryngologic infections. Curr Opin Otolaryngol Head Neck Surg 2004; 12(3): 185–90.
8. Mack D, Beckerb P, Chatterjeec I, Dobinskya S, Knoblocha JKM, Petersb G, et al. Mechanisms of biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus: functional molecules, regulatory circuits, and adaptive responses. Int J Med Microbiol 2004; 294(2): 203–12.
9. Kawamura H, Nishi J, Imuta N, Tokuda K, Miyanohara H, Hashigu‌chi T, et al. Quantitative analysis of biofilm formation of meth‌icillin-resistant Staphylococcus aureus (MRSA) strains from pa‌tients with orthopaedic device-related infections. FEMS Immunol Med Microbiol 2011; 63(1): 10-5.
10. Joo HS, Otto M. Molecular basis of in vivo biofilm formation by bacterial pathogens. Chem Biol 2012; 19(12): 1503–13.
11. Atshan SS, Shamsudin MN, Lung LT, Sekawi Z, Ghaznavi-Rad E, Pei CP. Comparative characterisation of genotypically different clones of MRSA in the production of biofilms. J Biomed Biotechnol 2012; 2012: 417247.
12. Sasirekha B, Usha MS, Amruta AJ, Ankit S. Evaluation and Com‌parison of Different Phenotypic Tests to Detect Methicillin Resis‌tant Staphylococcus aureus and their Biofilm Production. Int J Pharm Tech Res 2012; 4(2): 532-541.
13. Samie A, Shivambu N. Biofilm production and antibiotic suscep‌tibility profiles of Staphylococcus aureus isolated from HIV and AIDS patients in the Limpopo Province, South Africa. Afr J Biotech‌nol 2011; 10(65): 14625-36.
14. Moori Bakhtiari N, Jamshidian J, Khalafi E. Effect of Juglans regia Li. Stem Bark Hydroalcoholic Extract on Methicillin Resistant Staphylococcus aureus. Jundishapur J Nat Pharm Prod 2016; 11(1): e29095.
15. Stepanovic S, Vukovic D, Dakic I, Savic B, Svabic-Vlahovic M. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 2000; 40(2): 175–9.
16. Stepanović S, Vuković D, Hola V, Di Bonaventura G, Djukić S, Cirković I, et al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for for assessment of biofilm production by staphylococci. APMIS 2007; 115(8): 891–9.
17. Rezaei M, Moniri R, Mousavi SGA, Jabari Shiade M. Prevalence of Biofilm Formation Among Methicillin Resistance Staphylococ‌cus aureus Isolated From Nasal Carriers. Jundishapur J Microbiol; 6(6): e9601.
18. El-Shekh NA, Ayoub AM, El-Hendawy HH, Abada EA, Khalifa SY. In vitro Activity of some Antimicrobial Agents against Intact and Disrupted Biofilms of Staphylococci in the Indwelling Vascular Catheter Patients. World Appl Sci J 2010; 10(1): 108-20.
19. El-Feky MA, El-Rehewy MS, Hassan MA, Abolella HA, Abd El-Baky RM, Gad GF. Effect of ciprofloxacin and N-acetylcysteine on bacte‌rial adherence and biofilm formation on ureteral stent surfaces. Pol J Microbiol 2009; 58(3): 261-7.
20. Molina A, Del Campo R, Maiz L, Morosini MI, Lamas A, Baquero F, et al. High prevalence in cystic fibrosis patients of multiresistant hospital-acquired methicillin-resistant Staphylococcus aureus ST228-SCCmecI capable of biofilm formation. J Antimicrob Che‌mother 2008; 62(5): 961-7.
21. O'Neill E, Pozzi C, Houston P, Smyth D, Humphreys H, Robinson DA, et al. Association between methicillin susceptibility and bio‌film regulation in Staphylococcus aureus isolates from device-related infections. J Clin Microbiol 2007; 45(5): 1379-88.
22. Wang L, Yu F, Yang L, Li Q, Zeng XZ, Xu Y. Prevalence of virulence genes and bio‌film formation among Staphylococcus aureus clinical isolates associated with lower respiratory infection. Afr J Microbiol Res 2010; 4(23): 2566-9.

XML   Persian Abstract   Print

Volume 20, Issue 6 (Bimonthly 2017) Back to browse issues page