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:: Volume 20, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 20(6): 525-531 Back to browse issues page
Phenotypic evaluation of biofilm producing ability in Methicillin resistant Staphylococcus aureus
Naghmeh Moori-Bakhtiari *, Maryam Moslemi
Department of Microbiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, I. R. Iran. , n.moori@scu.ac.ir
Abstract:   (2190 Views)

Background: Like genomic changes, the ability for biofilm production is considered as one of the antibiotic resistant factors in bacteria which can cause recurrent infections.  The infection resulted from Methicillin-resistant staphylococcus aureus is the most common form of such complications manifested as recurrent infections. The aim of this study was to investigate biofilm production ability among isolated Methicillin-resistant Staphylococcus aureus (MRSA) in patients with different types of clinical infection.

Material and Methods: Fifty Staphylococcus aureus strains were isolated from different specimens and identified by biochemical and species-specific PCR tests. Methicillin- resistance specificity of isolates was confirmed by disk diffusion method and mecA gene presence; the biofilm- forming ability was evaluated by crystal violet microtiter plate assay and Congo red agar (CRA).

Results: Using turbidimetry with no acetic acid, the ability for biofim production was seen at 550 and 492 nm in 34 (68%) and 28 isolates (56%), respectively. In both methods, the most of isolates were weak biofilm producers. In CRA, 94% of isolates were biofilm producers which most (72.3%) of them were moderate producers.

Conclusions: While with the consideration of three studied methods high percentages of isolates were biofilm producers and despite the significant correlation seen between their results, there was a higher correlation coefficient between the results obtained from crystalviolet-treated microtiter plates with two reading methods.

Keywords: Methicillin-resistant staphylococcus aureus, Biofilm, Crystal violet microtiter plate, Congo red agar medium
Full-Text [PDF 233 kb]   (512 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/01/2 | Accepted: 2017/01/2 | Published: 2017/01/2
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Moori-Bakhtiari N, Moslemi M. Phenotypic evaluation of biofilm producing ability in Methicillin resistant Staphylococcus aureus . Feyz. 2017; 20 (6) :525-531
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