Non-invasive treatment of advanced atherosclerotic stenosis in the rabbit carotid artery using low-level combined dual- frequency ultrasonication
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Hosein Mehrad , Manijeh Mokhtari-Dizaji * , Hosein Ghanaati |
Tarbiat Modares University , mokhtarm@modares.ac.ir |
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Abstract: (5264 Views) |
Background: The present study aimed to investigate the effect of low-level combined dual- frequency of 1 MHz and 150 kHz ultrasonication on fibro-lipid plaque with neovascularization in the rabbit common carotid artery. Materials and Methods: In this experimental study, the common carotid arteries in 17 rabbits were injured perivascularly by liquid nitrogen, followed by a 1.5% cholesterol-rich diet for eight weeks. The animals were randomly divided into three groups including A: a cholesterol-rich diet (n=7) and evaluation at eighth week, B: control and discontinuation of cholesterol-rich diet (n=5) and evaluation at twelfth week and C: low-level combined dual- frequency ultrasonication and discontinuation of cholesterol-rich diet (n=7) and evaluation at twelfth week. Blood volume flow and blood mean velocity were measured by color Doppler ultrasonography. Moreover, mean wall thickness and percentage of luminal cross-sectional area of stenosis were measured by B-mode ultrasonographic and histological methods in the stenotic region of the common carotid artery. Results: Results showed a significant reduction in the mean value for blood peak systolic pressure, blood mean pressure, blood mean velocity, mean wall thickness and percentage of luminal cross-sectional area of stenosis and a significant increase in the mean value for blood peak diastolic pressure and blood volume flow in group C compared with the other groups (P<0.05). Conclusion: Enhanced inertial cavitation effect of low-level combined dual- frequency ultrasonication can cause the degradation of the microvessels and the fibro-lipid content, lipolysis and thrombolysis in the plaque and significantly dilate the luminal cross-sectional area of stenosis. |
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Keywords: Doppler ultrasonography, Carotid atherosclerosis, Low-level dual frequency ultrasonication, Cavitation, Histology |
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Full-Text [PDF 862 kb]
(5560 Downloads)
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Type of Study: Research |
Subject:
medicine, paraclinic Received: 2014/08/23 | Revised: 2014/09/13 | Accepted: 2014/08/23 | Published: 2014/08/23
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