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:: Volume 18, Issue 4 (Bimonthly 2014) ::
Feyz 2014, 18(4): 345-353 Back to browse issues page
The effect of intrathecal administration of Genipin on rat paw inflammatory edema in the presence and absence of morphine
Zohreh Abbasi, Masoud Fereidoni *, Morteza Behnam-Rasouli
Ferdowsi University of Mashhad , fereidoni@um.ac.ir
Abstract:   (1936 Views)

Background: Uncoupling protein 2 (UCP2) is an intramembranous mitochondrial protein that the knocking down of its gene causes an induction of the pro-inflammatory responses. However, genipin, as a pharmacological inhibitor of UCP2, has anti-inflammatory effects. In this study the effect of genipin on rat paw inflammatory edema induced by formalin in the presence and absence of morphine was investigated.

Materials and Methods: In this research, male Wistar rats (200-250g) were assigned to seven groups: Saline(i.p)-Saline(i.t), Saline(i.p)-DMSO(i.t), Saline(i.p)-genipin(i.t), Morphine (10mg/kg,i.p)-DMSO(i.t), Morphine(10mg/kg,i.p)-genipin(i.t), Morphine(1μg/kg,i.p)-DMSO(i.t) and Morphine(1μg/kg,i.p)-genipin(i.t). Inflammation was induced in the rat’s hind paw by the injection of 50 µl of formalin 2.5% and paw volume was measured using plethysmometer method before and after the injection.

Results: Results revealed an anti-inflammatory effect for genipin against the formalin-induced paw edema (P<0.001). Genipin potentiated the anti-inflammatory effects of morphine, whereas it had no effect on pro-inflammatory effects of low dose of morphine this dose of morphine reversed the anti-inflammatory effects of genipin.

Conclusion: Although genipin, a UCP2 inhibitor, was expected to increase the inflammatory edema, but probably its anti-inflammatory effects via the NF-κB inhibition is so robust that can cover its inhibitory effect on UCP2 and the subsequent inflammation. The anti-inflammatory effect of morphine was set in the range of anti-inflammatory effects of genipin. The finding that the anti-inflammatory effect of genipin is attenuated at the presence of ultra-low dose morphine may be due to their common mechanisms which suggest further research on KATP channels.

Keywords: UCP2, Genipin, Inflammation, Morphine
Full-Text [PDF 350 kb]   (633 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2014/08/16 | Accepted: 2014/08/16 | Published: 2014/08/16
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Abbasi Z, Fereidoni M, Behnam-Rasouli M. The effect of intrathecal administration of Genipin on rat paw inflammatory edema in the presence and absence of morphine. Feyz. 2014; 18 (4) :345-353
URL: http://feyz.kaums.ac.ir/article-1-2330-en.html


Volume 18, Issue 4 (Bimonthly 2014) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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