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:: Volume 17, Issue 2 (Bimonthly 2013) ::
Feyz 2013, 17(2): 181-187 Back to browse issues page
Identification of serological groups A, B, C, W135, Y, X Neisseria meningitidis carriers by multiplex PCR in the nasopharynx of students in Kashan during 2011-2012
Mahdi Valipour, Ahmad Piroozmand, Ahmad Khorshidi *, Hossein Akbari, Hamed Mirzaee
Kashan University of Medical Sciences , khorshidi_a@kaums.ac.ir
Abstract:   (5274 Views)

Background: Neisseria meningitidis (N. meningitidis) is a pathogen which colonizes in the nasopharynx without any clinical manifestations. Among the 13 different serological groups, only the serogroups A, B, C, W135, Y, X play a major role in disease development. This study aimed to determine N. meningitidis cases carrying these serological groups using the multiplex PCR method in the nasopharynx of students in Kashan schools .

Materials and Methods: This cross-sectional study was conducted on 1289 students in Kashan during 2011-2012. Samples were collected from the students' nasopharynx using a sterile swab and cultured on a selective medium . Strains were identified through biochemical tests. Then the serological groups were determined using the multiplex PCR method.

Results: One-hundred and fifteen (8.9%) out of 1289 students were N.meningitidis carriers 75 (65.2%) male and 40 (34.8%) female. There was a significant difference between gender and the rate of carriers (P=0.032). The highest rate of carriers (12.3%) was in the 15 to 19 year age group. There was a significant relationship between the rate of carriers and increase in the number of family members (P<0.001). In this study, only the serological groups B (8 cases) and C (107 cases) were detected.

Conclusion: Since the serological group C is involved in the outbreak and there is no vaccine currently available for the serological group B to prevent the infection, detection of these serological groups can be important.

Keywords: N.meningitidis, Carriers, Nasopharynx, Multiplex PCR, Serological groups
Full-Text [PDF 265 kb]   (1739 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/05/7 | Accepted: 2013/05/15 | Published: 2013/05/15
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Valipour M, Piroozmand A, Khorshidi A, Akbari H, Mirzaee H. Identification of serological groups A, B, C, W135, Y, X Neisseria meningitidis carriers by multiplex PCR in the nasopharynx of students in Kashan during 2011-2012. Feyz. 2013; 17 (2) :181-187
URL: http://feyz.kaums.ac.ir/article-1-1911-en.html


Volume 17, Issue 2 (Bimonthly 2013) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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