[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 17, Issue 2 (Bimonthly 2013) ::
Feyz 2013, 17(2): 181-187 Back to browse issues page
Identification of serological groups A, B, C, W135, Y, X Neisseria meningitidis carriers by multiplex PCR in the nasopharynx of students in Kashan during 2011-2012
Mahdi Valipour , Ahmad Piroozmand , Ahmad Khorshidi , Hossein Akbari , Hamed Mirzaee
Kashan University of Medical Sciences , khorshidi_a@kaums.ac.ir
Abstract:   (6782 Views)

Background: Neisseria meningitidis (N. meningitidis) is a pathogen which colonizes in the nasopharynx without any clinical manifestations. Among the 13 different serological groups, only the serogroups A, B, C, W135, Y, X play a major role in disease development. This study aimed to determine N. meningitidis cases carrying these serological groups using the multiplex PCR method in the nasopharynx of students in Kashan schools .

Materials and Methods: This cross-sectional study was conducted on 1289 students in Kashan during 2011-2012. Samples were collected from the students' nasopharynx using a sterile swab and cultured on a selective medium . Strains were identified through biochemical tests. Then the serological groups were determined using the multiplex PCR method.

Results: One-hundred and fifteen (8.9%) out of 1289 students were N.meningitidis carriers 75 (65.2%) male and 40 (34.8%) female. There was a significant difference between gender and the rate of carriers (P=0.032). The highest rate of carriers (12.3%) was in the 15 to 19 year age group. There was a significant relationship between the rate of carriers and increase in the number of family members (P<0.001). In this study, only the serological groups B (8 cases) and C (107 cases) were detected.

Conclusion: Since the serological group C is involved in the outbreak and there is no vaccine currently available for the serological group B to prevent the infection, detection of these serological groups can be important.

Keywords: N.meningitidis, Carriers, Nasopharynx, Multiplex PCR, Serological groups
Full-Text [PDF 265 kb]   (2583 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/05/7 | Revised: 2013/05/15 | Accepted: 2013/05/15 | Published: 2013/05/15
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Valipour M, Piroozmand A, Khorshidi A, Akbari H, Mirzaee H. Identification of serological groups A, B, C, W135, Y, X Neisseria meningitidis carriers by multiplex PCR in the nasopharynx of students in Kashan during 2011-2012. Feyz 2013; 17 (2) :181-187
URL: http://feyz.kaums.ac.ir/article-1-1911-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 17, Issue 2 (Bimonthly 2013) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645