[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 16, Issue 4 (Bimonthly 2012) ::
Feyz 2012, 16(4): 317-323 Back to browse issues page
Evaluating the immunogenicity for plasmid encoding GRA5 antigen of Toxoplasma gondii in BALB/c mice
Razi Naserifar , Fatemeh Ghaffarifar * , Abdolhossein Dalimi-Asl , Zohreh Sharifi , Saeedeh Shojaei , Mahbobeh Salimi
Tarbiat Modares University , ghafarif@modares.ac.ir
Abstract:   (6396 Views)

Background: Severe or lethal damages of toxoplasmosis clearly indicate the need for the development of a more effective vaccine. Immunization with recombinant plasmid encoding protective proteins is a promising vaccination technique. Therefore, this study aimed to evaluate the immunization with plasmid encoding GRA5 antigen of Toxoplasma gondii in BALB/c mice.

Materials and Methods: In this experimental study, three groups of BALB/c mice (n=10 in each group) were selected using simple random sampling. GRA5 gene was cloned into pcDNA3 plasmid and purified by plasmid purification kits and then the product was injected (IM). To determine the status of cellular and humoral immunity, the Il-4, IFN-y and IgG, IgG2a, IgG subtypes were evaluated respectively using the ELISA-based assay.

Results: The group immunized with pcGRA5 indicated a significant augmented response in humoral and cellular immunity (P≤0.05) which was confirmed by MTT test. The mean survival time for the experimental and control groups were 9 and 6 days, respectively.

Conclusion: The immunized mice by pcGRA5 produce the higher titers of IFNγ indicated a Th1 response which is confirmed by the high level of IgG2a. Findings of this study demonstrate that GRA5 gene of T. gondii can be a potential vaccine candidate against the toxoplasmosis.

Keywords: Toxoplasma gondii, DNA Vaccine, Cellular immunity, Humoral immunity, GRA5 antigen
Full-Text [PDF 287 kb]   (1522 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2012/07/25
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Naserifar R, Ghaffarifar F, Dalimi-Asl A, Sharifi Z, Shojaei S, Salimi M. Evaluating the immunogenicity for plasmid encoding GRA5 antigen of Toxoplasma gondii in BALB/c mice. Feyz. 2012; 16 (4) :317-323
URL: http://feyz.kaums.ac.ir/article-1-1552-en.html


Volume 16, Issue 4 (Bimonthly 2012) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3772