:: Volume 9, Issue 1 (Quarterly 2005) ::
Feyz 2005, 9(1): 45-49 Back to browse issues page
Relevance of RIBA-3 Complementary Test to HCV RT-Positivity
Sedighe Amini Kafiabad *, Ali Talebian, Zahra Ataii, Fahime Ranjbar Karmani
Abstract:   (7917 Views)

Background: Hepatitis C virus (HCV) is the predominant cause of nonA-nonB and post transfusion hepatitis. This study was conducted to evaluate the efficacy of third generation Recombinant Immunoblot Assay (RIBA-3) especially structural and non-structural bands compared with Reverse Transcripatase Polymerase Chain Reactions (RT-PCR) for the diagnosis of hepatitis C infection.

Materials and Methods: Sera of 80 patients with ELIAS-3 positive were analyzed by RIBA-3 and RT-PCR.

Results: Forty-eight out of 80 patients were positive in RIBA-3. Of them 92% was RT-PCR positive and only 4 patients were RN negative. Among 20 samples with 4 bands, 18 (90%) were RNA positive. Among the 18, 3-bands positive, 16(88%) were RNA positive, whereas all of 11(100%) of RIBA-3 positive with anti-capsid and anti-NS3 reactivity are RNA positive. Among the RIBA-3 indeterminate cases (13 patients), HCV-RNA was positive only in 31 % (4 patients). Sera of these four patients were reactive with the core (C22) region in RIBA-3. All samples reacted to NS-4 regions were RT-PCR negative. So samples with indeterminate results in RIBA-3 should be evaluated by RT-PCR for HCV RNA. All samples without any reaction in RIBA-3 were RT-PCR negative.

Conclusion: There were not absolute correlations between the results of RIBA-3 and PCR but all of RIBA-3 positvie with anti-capsid and anti-NS3 reactivity were PCR positive, but other profiles were associated with HCV viremia in about 88%-90% of cases.

Keywords: Hepatitis C Virus, RIBA-3, RT-PCR, Structural and non-structural bands
Full-Text [PDF 192 kb]   (7402 Downloads)    
Type of Study: Research | Subject: General
Received: 2008/11/1 | Published: 2005/04/15


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Volume 9, Issue 1 (Quarterly 2005) Back to browse issues page