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:: Volume 30, Issue 3 (Bimonthly 2026) ::
Feyz Med Sci J 2026, 30(3): 281-297 Back to browse issues page
Synergistic Protective Effects of Hydroalcoholic Extract of Echinophora platyloba and Vitamin E against Testicular Ischemia–Reperfusion Injury
Ashkan Sadeghi , Mehdi Farid , Elham Moghtadaei-Khorasgani * , Mehrdad Yadegari , Moosa Javdani
Department of Pathobiology, ShK.C., Islamic Azad University, ShahreKord, Iran , Moghtadaiee@gmail.com
Abstract:   (437 Views)
Background and Aim: Testicular ischemia–reperfusion (I/R) injury is a major cause of male infertility, primarily mediated by excessive reactive oxygen species (ROS) production and disruption of the antioxidant defense system. This study aimed to investigate the synergistic effects of hydroalcoholic extract of Echinophora platyloba and Vitamin E on Doppler ultrasound indices, oxidative stress markers, antioxidant gene expression, sperm count, and testosterone levels in a rat model of testicular I/R injury.
Methods: In this experimental study, 30 adult male Wistar rats were randomly assigned to five groups: Sham, torsion–detorsion (T/D), T/D + E. platyloba extract (400 mg/kg), T/D + Vitamin E (160 mg/kg), and T/D + combination therapy. Color Doppler ultrasonography parameters, including echogenicity, resistance index, and testicular volume, were assessed on days 1 and 21. Malondialdehyde (MDA), total antioxidant capacity (TAC), SOD and CAT enzyme activities, and testosterone levels were measured using spectrophotometric, enzymatic, and ELISA methods. SOD and CAT gene expression was evaluated by Real-Time PCR.
Results: The T/D group exhibited increased oxidative stress and reduced antioxidant activity. Combination therapy resulted in a 44% reduction in MDA levels and a 2.67-fold increase in TAC compared with the T/D group (P<0.05). Moreover, SOD and CAT gene expressions increased by 1.7-fold and 4.7-fold, respectively. Sperm count improved by 4.7-fold, and Doppler ultrasound parameters and testosterone levels approached those observed in the Sham group.
Conclusion: Co-administration of E. platyloba extract and vitamin E enhanced antioxidant capacity and modulated SOD and CAT gene expression, providing significant protective effects against testicular I/R injury. This combination improved sperm parameters and testosterone levels and may represent a potential adjunctive approach for preserving fertility following testicular torsion in future preclinical studies.
Keywords: Echinophora platyloba, Vitamin E, Sperm, Oxidative stress, Doppler ultrasound, Ischemia-reperfusion
Full-Text [PDF 1562 kb]   (77 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2026/05/27 | Revised: 2026/09/2 | Accepted: 2026/07/13 | Published: 2026/08/23
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Sadeghi A, Farid M, Moghtadaei-Khorasgani E, Yadegari M, Javdani M. Synergistic Protective Effects of Hydroalcoholic Extract of Echinophora platyloba and Vitamin E against Testicular Ischemia–Reperfusion Injury. Feyz Med Sci J 2026; 30 (3) :281-297
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