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The effect of aerobic exercise followed by footshock electrical stimulation on ACTH and GnRH gene expression in brain tissue of obese rats with calorie restriction
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Alireza Bagheri    , Mehdy Morady * , Mohammad Malekipooya , Bahram Abedi |
| Department of Exercise Physiology, Ar.C., Islamic Azad University, Arak, Iran. , mehdy_morady59@yahoo.com |
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Abstract: (35 Views) |
Introduction: Obesity is a multifactorial disease that affects important aspects of human health and is directly related to hormone secretion. Hormones play a key role in regulating appetite, metabolism, and fat distribution in the body. Therefore, the aim of the present study was to investigate the effect of aerobic exercise followed by shock stimulation on ACTH and GnRH gene expression in the brain tissue of calorie restriction (fasted) obese rats.
Materials & Methods: In this experimental study, with a control group of 35 male Wistar rats (8 weeks old, weighing 200±19g), after induction of obesity and weight gain, randomly divided into 5 groups of 8: control, fasting-obese, fasting-endurance exercise, fasting-electrical stimulation and Fasting-endurance training-electrical stimulation were divided. Intervention groups for a period of 4 weeks under endurance sports activity (with a speed of 10 to 20 m/min and duration of 20 to 40 minutes), electrical stimulation (foot shock device for 0.5 mA and 20 minutes) and fasting (8 to 16 hours) were placed. After exercise and anesthesia, brain tissue sampling was done and after molecular processes, gene expression was measured using Real time-PCR machine. For data analysis, two-way analysis of variance test was used at a significance level of p<0.05 and GraphPad software.
Results: The results showed that endurance training significantly increased the expression of ACTH and GnRH genes in fasted obese rats compared to all groups (P=0.0001). Also, electrical stimulation was associated with a significant increase, especially compared to the fasted obese group, in each marker (P=0.0001). Electrical stimulation and its combination also showed a significant increase in GnRH gene expression compared to the fasted group (P=0.0001).
Conclusion: It seems that endurance training and electrical stimulation during fasting were effective in increasing ACTH and GnRH expression in obese samples with hormonal regulation in their physical, sexual health, and fat distribution.
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| Keywords: Obesity, Fasting, Endurance exercise, Electrical stimulation, ACTH, GnRH |
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Type of Study: Research |
Subject:
General
Received: 2025/09/18 | Revised: 2025/12/1 | Accepted: 2025/12/1
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