|
The effect of a period of aerobic exercise combined with movement on the expression of caspase 3 and 9 genes in muscle tissue of obese rats with calorie restriction
|
Hasan Sharifi   , Mohamad Malekipooya * , Mehdi Roozbahani , Mehdy Morady , Mojtaba Khansooz |
| Department of Exercise Physiology, Ar.C., Islamic Azad University, Arak, Iran , Mo.malekipooya@iau.ac.ir |
|
|
Abstract: (9 Views) |
Background and Aim: Excessive consumption of high-fat diets is associated with impaired cell function, inflammation, mitochondrial damage, and upregulation of apoptotic proteins. In contrast, aerobic exercise and electrical stimulation can provide the necessary adaptations to inhibit and stop apoptosis. The aim of the present study was to investigate the effect of a period of aerobic exercise combined with electrical stimulation on the expression of caspase 3 and 9 genes in muscle tissue of obese rats with calorie restriction.
Methods: In this experimental study, 50 male Wistar rats were randomly divided into 5 groups of 10, including aerobic exercise, electrical stimulation, aerobic exercise-electrical stimulation, calorie-restricted control, and non-calorie-restricted control. After obesity induction (with a high-fat diet, peanuts, milk chocolate, and sweet biscuits in a ratio of 3:2:2:1), aerobic exercise was performed for 5 sessions per week, 60 minutes at an intensity of 55-60% Vo2max and 20 minutes of electrical stimulation for 4 weeks. Then, Real Time PCR was used to determine the expression of the desired genes. Two-way analysis of variance and Tukey's post hoc test were used to analyze the data.
Results: Statistical analysis showed a significant decrease in caspase-3 expression in the exercise-electrical stimulation group compared to the exercise, electrical stimulation, calorie-restricted control group, and no calorie restriction (P<0.0001). The expression level of caspase-9 in the exercise-electrical stimulation group compared to the electrical stimulation, fasted control, and no control groups showed a significant increase (P<0.0001).
Conclusion: Exercise and electrical stimulation, each alone and together, have different effects on the expression of caspase 3 and caspase 9. It is expected that this type of exercise can provide a suitable platform for caspase mechanisms in rats.
|
|
| Keywords: Aerobic exercise, electrical stimulation, caspase 3, caspase 9, obese |
|
|
|
Type of Study: Research |
Subject:
medicine, paraclinic
Received: 2025/08/7 | Revised: 2025/11/3 | Accepted: 2025/10/25
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
_-_Copy.png)
.png){kind=logo}
