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The effect of eight weeks of High intensity interval training along with Gallic acid on mTOR and ULK1 in kidney tissue of rats exposed to cadmium
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Maryam Zare    , Ahmad Hemat far * , Seyedd ali Hosseini , Arash Sameni |
| Borujerd Branch, Islamic Azad University, Borujerd, Iran , ahematfar@yahoo.com |
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Abstract: (67 Views) |
Background and Aims: Cadmium (Cd) as a toxic metal has irreparable effects on the kidney. Therefore, the aim of the present study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and Gallic acid (GA) on mTOR and ULK1 in kidney tissue of rats exposed to Cd.
Methods: 42 male Sprague-Dawley rats aged 8-9 months and weighing 250-270 grams were randomly divided to healthy control group (HC), Cd (5 mg/kg/day), sham, Cd+GA, Cd+HIIT and Cd+HIIT+GA groups. The supplement groups received 20 mg/kg/day GA dissolved in food flavoring and dissolved water orally. The exercise groups also performed HIIT exercises with an intensity of 80-110% of Vo2max for eight weeks, three sessions a week. Then, the gene expression of mTOR and ULK1 were used using q Teal Time PCR method.
Results: The gene expression of mTORC1 was significantly lower in the Cd group, while ULK1 expression was significantly higher compared to the HC group (P=0.001). In the Cd+GA (P=0.001), Cd+HIIT (P=0.001), and Cd+HIIT+GA (P=0.001) groups, mTORC1 gene expression was significantly higher, whereas ULK1 levels in these groups were lower than those in the Cd group. Additionally, the mTORC1 gene expression in the Cd+HIIT+GA group was significantly higher, and ULK1 levels in this group were lower than those in the Cd+GA (P=0.001) and Cd+HIIT (P=0.001) groups. The results indicated that ULK1 levels in the Cd+GA group were significantly lower than in the Cd+HIIT group (P=0.004).
Conclusion: It seems that although HIIT and GA are both effective in improving gene expression studied in kidney tissue following cadmium-induced kidney damage; But due to the nature of reinforcing each other's effect, the combination of exercise and GA has a more favorable effect on mTORC1 and ULK1 in kidney tissue. |
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| Keywords: High Intensity Interval Training, Gallic acid, Autophagy, Kidney, Cadmium |
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Type of Study: Research |
Subject:
General
Received: 2024/12/9 | Revised: 2025/08/3 | Accepted: 2025/07/30
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