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:: Volume 28, Issue 6 (Bimonthly 2024) ::
Feyz Med Sci J 2024, 28(6): 574-586 Back to browse issues page
Effects of Hemiscorpius lepturus scorpion venom fractions on cytokine release in male rats
Daniel Shaebannia , Mohammad Razi jalali * , Ali Shahrirari , Hedieh Jafari , Masoumeh Ahmadi zadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran , jalali_m@scu.ac.ir
Abstract:   (321 Views)
Background and Aim: The secretion and activation of cytokines play a significant role in the pathogenesis of scorpion venom. This study aimed to investigate the effects of Hemiscorpius lepturus scorpion venom fractions on the release of IL-1, IL-6, and TNF-α cytokines in male rats.
Methods: In this study, scorpion venom was divided into six fractions using chromatography. A total of 180 male rats were randomly assigned to 15 equal groups, and the fractions were administered at two doses of 1000 µg/kg and 3000 µg/kg (equivalent to 1/2 and 3/1 LD50). The groups included one control group, one group treated with raw venom at a dose of 1000 µg/kg, one group treated with raw venom at a dose of 3000 µg/kg, six groups treated with venom fractions at 1000 µg/kg, and six groups treated with venom fractions at 3000 µg/kg. All treatments were administered intraperitoneally. Blood samples were collected at 1, 3, 6, and 24 hours post-injection, and cytokine levels were measured using laboratory kits.
Results: The results showed that the activity of all cytokines was significantly higher in the raw venom groups compared to the control group and other experimental groups (P<0.05). The highest effect was observed in the raw venom group, followed by fraction 2, and then fraction 6, which showed the most significant impact.
Conclusion: Fraction 2 of Hemiscorpius lepturus scorpion venom appears to be the most important component responsible for inducing inflammation and inflammatory symptoms in the body.
Keywords: Scorpion Venom, Fraction, Hemiscorpius lepturus, IL-1, IL-6, TNF-α
Full-Text [PDF 664 kb]   (75 Downloads)    
Type of Study: Research | Subject: General
Received: 2024/11/7 | Revised: 2025/02/5 | Accepted: 2025/01/12 | Published: 2025/02/5
References
1. Ghafourian M, Ganjalikhanhakemi N, Hemmati AA, Dehghani R, Kooti W. The effect of Hemiscorpius lepturus (Scorpionida: Hemiscorpiidae) venom on leukocytes and the leukocyte subgroups in peripheral blood of rat. J Arthropod Borne Dis. 2016; 10(2): 159-67.
2. Dehghani R, Djadid ND, Shahbazzadeh D, Bigdelli S. Introducing Compsobuthus matthiesseni (Birula, 1905) scorpion as one of the major stinging scorpions in Khuzestan, Iran. Toxicon. 2009; 54(3): 272-5. doi:10.1016/j.toxicon.2009.04.011 PMid:19393258
3. Shahbazzadeh D, Srairi-Abid N, Feng W, Ram N, Borchani L, Ronjat, et al.,Hemicalcin, a new toxin from the Iranian scorpion Hemiscorpius lepturus which is active on ryanodine-sensitive Ca2+ channels. Biochem J. 2007; 404(1): 89-96. doi:10.1042/BJ20061404 PMid:17291197 PMCid:PMC1868827
4. Pipelzadeh MH, Jalali A, Taraz M, Pourabbas R, Zaremirakabadi A. An epidemiological and a clinical study on scorpionism by the Iranian scorpion Hemiscorpius lepturus. Toxicon. 2007; 50(7): 984-92. doi:10.1016/j.toxicon.2007.07.018 PMid:17854855
5. Valavi E, Ansari MA. Hemolytic uremic syndrome following Hemiscorpius lepturus (scorpion) sting. Indian J Nephrol. 2008; 18(4): 166-8. doi:10.4103/0971-4065.45293 PMid:20142930 PMCid:PMC2813541
6. Heidarpour M, Ennaifer E, Ahari H, Srairi-Abid N, Borchani L, Khalili G, et al. Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice. Toxicon. 2012; 59(3): 373-8. doi:10.1016/j.toxicon.2011.12.011 PMid:22230352
7. Seyedian R, Pipelzadeh MH, Jalali A, Kim E, Lee H, Kang C, et al. Enzymatic analysis of Hemiscorpius lepturus scorpion venom using zymography and venom-specific antivenin. Toxicon. 2010; 56(4): 521-5. doi:10.1016/j.toxicon.2010.05.008 PMid:20493200
8. Abdoon NA, Fatani AJ. Correlation between blood pressure, cytokines and nitric oxide in conscious rabbits injected with Leiurus quinquestriatus quinquestriatus scorpion venom. Toxicon. 2009; 54(4): 471-80. doi:10.1016/j.toxicon.2009.05.009 PMid:19467253
9. Khodadadi A, Pipelzadeh MH, Vazirianzadeh B, Pipelzadeh M, Sharifat M. An in vitro comparative study upon the toxic properties of the venoms from Hemiscorpius lepturus, Androctonus crassicauda and Mesobuthus eupeus scorpions. Toxicon. 2012; 60(3): 385-90. doi:10.1016/j.toxicon.2012.04.348 PMid:22569320
10. Mirdehghan M, Motlagh Me, Chomeili B. A 5 Year Study Of Scorpion Stings In Children Referred To Abuzar Pediatric Center In Ahvaz. Stud Med Sci. 2001; 12(2): 138-46.
11. Dorce AL, Frare EO, Paulo ME, Dorce VA, Nencioni AL. Tityus bahiensis scorpion venom injected to dams during pregnancy affects some cytokines of fetuses. Toxicon. 2015; 103:114-8. doi:10.1016/j.toxicon.2015.06.023 PMid:26140840
12. Jalali MR, Fatemi SR, Shirali T. Comparative study on the effects of Hemiscorpius lepturus, Hottentotta saulcyi and Mesobuthus eupeus scorpions on blood hemostasis in rats. Bio Cell Arch. 2011; 11(1): 125-9.
13. Yazdkhati M, Razi-Jalali M, Khadjeh GH, Jafari H, Rezaei A. Cardiotoxic Effects of Hemiscorpius Lepturus Scorpion Venom Fractions in Rats. Iran J Toxic. 2021; 15: 27-36. doi:10.32598/IJT.15.1.691.1
14. Coelho JS, Ishikawa EA, Santos PR, Pardal PP. Scorpionism by Tityus silvestris in eastern Brazilian Amazon. J Venom Anim Toxins Incl Trop Dis. 2016; 22(1): 24. doi:10.1186/s40409-016-0079-2 PMid:27570532 PMCid:PMC5000479
15. Laaradia MA, El Hidan MA, Marhoume F, Bouimeja B, Oufquir S, Sokar Z, et al. Buthus lienhardi venom and pathophysiological effects at the histological, hematological, biochemical and motor skills levels. Toxicon. 2018; 1(146): 106-113.doi:10.1016/j.toxicon.2018.03.001 PMid:29526539
16. Sifi A, Adi-Bessalem S, Laraba-Djebari F. Role of angiotensin II and angiotensin type-1 receptor in scorpion venom-induced cardiac and aortic tissue inflammation. Exp Mol Pathol. 2017; 102(1): 32-40. doi:10.1016/j.yexmp.2016.11.006 PMid:27955986
17. Petricevich VL, Cruz AH, Coronas FI, Possani LD. Toxin gamma from Tityus serrulatus scorpion venom plays an essential role in immunomodulation of macrophages. Toxicon. 2007; 50(5): 666-75. doi:10.1016/j.toxicon.2007.06.001 PMid:17675201
18. De Oliveira Yamashita F, Torres-Rêgo, M, Dos Santos Gomes JA, Félix-Silva J, Passos JGR, De Santis Ferreira L, et al. Mangaba (Hancornia speciosa Gomes) fruit juice decreases acute pulmonary edema induced by Tityus serrulatus venom: Potential application for auxiliary treatment of scorpion stings. Toxicon. 2020; 179: 42-52. doi:10.1016/j.toxicon.2020.02.025 PMid:32174508
19. Meki AR, El-Deen ZM, El-Deen HM. Myocardial injury in scorpion envenomed children: significance of assessment of serum troponin I and interleukin-8. Neuro Endocrinol Lett. 2002; 23(2): 133-40. doi:10.1016/S0041-0101(02)00155-1 PMid:12565731
20. Kofler S, Nickel T, Weis M. Role of cytokines in cardiovascular diseases: a focus on endothelial responses to inflammation. Cli Sci. 2005; 108(3): 205-13. doi:10.1042/CS20040174 PMid:15540988
21. Jalali MR, Jalali MT, Mapar Z. Evaluation of plasma cytokine levels in Mesobuthus Eupeus (Scorpionida: Buthidae) scorpion envenomation in rats treated with polyvalent antivenom. Jundishapur J Health Sci. 2015; 7(1). doi:10.5812/jjhs.27159
22. Hadaddezfuli R, Khodadadi A, Assarehzadegan MA, Pipelzadeh MH, Saadi S. Hemiscorpius lepturus venom induces expression and production of interluckin-12 in human monocytes. Toxicon. 2015; 100: 27-31. doi:10.1016/j.toxicon.2015.04.007 PMid:25912944
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Shaebannia D, Razi jalali M, Shahrirari A, Jafari H, Ahmadi zadeh M. Effects of Hemiscorpius lepturus scorpion venom fractions on cytokine release in male rats. Feyz Med Sci J 2024; 28 (6) :574-586
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Volume 28, Issue 6 (Bimonthly 2024) Back to browse issues page
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