Changes in protein expression of NMDA glutamate receptor and Pannexin-1 channel in gastric antrum tissue of rats with type 2 diabetes and treated with insulin

Rezazadeh Mehrizi, Hossein; Soltani, Nepton; Mehranfard, Nasrin; Ghasemi, Maedeh

doi:10.48307/FMSJ.2023.0.5.473

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Volume 27, Issue 5 (Bimonthly 2023)

Feyz Med Sci J 2023, 27(5): 473-480

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Changes in protein expression of NMDA glutamate receptor and Pannexin-1 channel in gastric antrum tissue of rats with type 2 diabetes and treated with insulin

Hossein Rezazadeh Mehrizi

, Nepton Soltani

, Nasrin Mehranfard

, Maedeh Ghasemi ^*

Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , ghasemi.m@med.mui.ac.ir

Abstract: (693 Views)

Background and Aim: Evidence shows that glutamate receptors play a role in the occurrence of gastrointestinal damage caused by diabetes. Therefore, in this study, the protein expression pattern of glutamate receptor N-methyl-D-aspartate (NMDA) and pannexin-1 (Panx-1) channel, which has an important functional relationship with glutamate receptors, in the stomach of type 2 diabetic rats were investigated. We also assessed the effect of long-term treatment with insulin on the possible changes of the mentioned proteins.
Methods: In this experimental study, 18 male Wistar rats weighing approximately 80-90 grams were randomly assigned into 3 groups (6 rats each): 1) control with standard diet, 2) type 2 diabetes: high-fat diet for 3 months + single dose of streptozotocin (35 mg/kg), and 3) type 2 diabetes + insulin treatment. The protein content of NMDA receptor as well as Panx-1 was detected using western blot method and compared among groups.
Results: In the stomach of type 2 diabetic rats, the expression of Panx-1 protein was significantly decreased (P<0.001), while the expression of NMDA receptor (P<0.001) showed a significant increase. Insulin treatment improved the expression level of Panx-1 protein in diabetic rats, in addition, insulin treatment decreased the expression level of NMDA receptor in diabetic rats (P<0.05).
Conclusion: The present findings indicate important changes in protein levels of NMDA receptor and Panx-1 channel and their possible role in the occurrence of sensory-motor and secretory damage related to diabetes. The correction of the above changes may play a role in improving the functional damage caused by type 2 diabetes.

Keywords: Type 2 diabetes, NMDA receptor, Pannexin-1 channel

Full-Text [PDF 503 kb] (486 Downloads)

Type of Study: Research | Subject: medicine, paraclinic
Received: 2023/08/6 | Revised: 2023/12/13 | Accepted: 2023/10/17 | Published: 2023/12/10

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Rezazadeh Mehrizi H, Soltani N, Mehranfard N, Ghasemi M. Changes in protein expression of NMDA glutamate receptor and Pannexin-1 channel in gastric antrum tissue of rats with type 2 diabetes and treated with insulin. Feyz Med Sci J 2023; 27 (5) :473-480
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