[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 26, Issue 2 (Bimonthly 2022) ::
Feyz 2022, 26(2): 118-127 Back to browse issues page
Evaluation of miR 26b 5p changes during differentiation of human endometrial stem cells into cardiomyocytes
Somayeh Sadat , Behrang Alani , Mahdi Noureddini , Behnaz Maleki , Alireza Farrokhian , Javad Verdi , Hossein Ghanbarian
Department of Applied Cell Sciences, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran. , Alani-be@kaums.ac.ir
Abstract:   (1212 Views)
Background: Recently, menstrual blood-derived stem cells as a unique source of stem cells with some features such as ease of access, high ability to proliferate and regenerate, lack of immune system stimulation and no tumorigenesis have raised great hopes for heart disease cell therapy. However, the regulatory mechanisms and role of miRNAs in controlling the differentiation of stem cells into cardiomyocytes are not fully understood. In this study, the level of human miR-26b-5p microRNA were investigated before and after differentiation of endometrial stem cells into heart cardiomyocytes.
Materials and Methods: Endometrial mesenchymal stem cells were differentiated into cardiomyocyte-like cells for 30 days in the presence of 5-azacitidine and fibroblast growth factor. Then, using bioinformatics studies, human miR-26b-5p microRNA was selected and its expression pattern was performed during days 0, 6, 12, 18, 24 and 30 of differentiation by Real Time qRT PCR
Results: Expression Level of human miR-26b-5p showed an uptrend differentiation between days 0 and 6 and then showed a significant decreasing trend differentiation from day 6 to day 18 and an uptrend again after day 18.
Conclusion: The non-uniformity in the expression of hsa-miR-26b-5p microRNA during the 24-day differentiation induction period indicates the existence of different messaging pathways involved in the differentiation process as well as different phases in the evolution and differentiation of cardiomyocytes. The miRNAs involved in the differentiation process and their possible role in turning off and on these messaging pathways at the beginning or end of a phase seems necessary.

 
Keywords: Endometrial mesenchymal stem cells, Cardiomyocyte, Human MIRN26A microRNA, Cell differentiation
Full-Text [PDF 538 kb]   (696 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/02/1 | Revised: 2022/07/4 | Accepted: 2022/05/1 | Published: 2022/05/30
References
1. Yester J, Kühn B. Mechanisms of Cardiomyocyte Proliferation and Differentiation in Development and Regeneration. Curr Cardiol Rep 2017; 19(2): 13-.
2. Liu Y, Niu R, Li W, Lin J, Stamm C, Steinhoff G, et al. Therapeutic potential of menstrual blood-derived endometrial stem cells in cardiac diseases. Cell Mol Life Sci 2019; 76(9): 1681-95.
3. Guo X, Bai Y, Zhang L, Zhang B, Zagidullin N, Carvalho K, et al. Cardiomyocyte differentiation of mesenchymal stem cells from bone marrow: new regulators and its implications. Stem Cell Res The 2018; 9(1): 1-12.
4. Kazemzadeh N, Sarvari MH, Jani MR, Razmara MR. Stem Cells Therapy For Peripheral Arterial Disorders. iranian journal of cardiovascular nursing. 2015; 3(4): 64- 73. [in Persian]
5. Bozorgmehr M, Gurung S, Darzi S, Nikoo S, Kazemnejad S, Zarnani AH, et al. Endometrial and menstrual blood mesenchymal stem/stromal cells: biological properties and clinical application. Front Cell Dev Biol 2020; 8.
6. Darzi S, Werkmeister JA, Deane JA, Gargett CE. Identification and characterization of human endometrial mesenchymal stem/stromal cells and their potential for cellular therapy. Stem Cells Transl Med 2016; 5(9): 1127-32.
7. Guo Y, Yu Y, Hu S, Chen Y, Shen Z. The therapeutic potential of mesenchymal stem cells for cardiovascular diseases. Cell Death Dis 2020; 11(5): 1-10.
8. Rahimi M, Zarnani AH, Mohseni-Kouchesfehani H, Kazemnejad S. Evaluation of differentiation potential of menstrual blood-derived stem cells to cardiomyocytes in vitro. Daneshvar Med 2016; 23(123): 31-42. [in Persian]
9. Szaraz P, Gratch YS, Iqbal F, Librach CL. In vitro differentiation of human mesenchymal stem cells into functional cardiomyocyte-like cells. J Vis Exp 2017; (126): e55757.
10. Paige SL, Plonowska K, Xu A, Wu SM. Molecular regulation of cardiomyocyte differentiation. Circ Res 2015; 116(2): 341-53.
11. Sun X, Li H, Zhu Y, Xu P, Zuo Q, Li B, et al. 5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells. Stem Cells Int 2020; 2020: 5162350.
12. Wang J, Greene SB, Bonilla-Claudio M, Tao Y, Zhang J, Bai Y, et al. Bmp signaling regulates myocardial differentiation from cardiac progenitors through a MicroRNA-mediated mechanism. Dev Cell 2010; 19(6): 903-12.
13. Alfar EA, El-Armouche A, Guan K. MicroRNAs in cardiomyocyte differentiation and maturation. Oxford University Press; 2018.
14. Katz MG, Fargnoli AS, Kendle AP, Hajjar RJ, Bridges CR. The role of microRNAs in cardiac development and regenerative capacity. Am J Physiol Heart Circ Physiol 2016; 310(5): H528-H41.
15. Wang Q, Xu C, Zhao Y, Xu Z, Zhang Y, Jiang J, et al. miR-26b-3p regulates human umbilical cord-derived mesenchymal stem cell proliferation by targeting estrogen receptor. Stem Cells Dev 2016; 25(5): 415-26.
16. Leeper NJ, Raiesdana A, Kojima Y, Chun HJ, Azuma J, Maegdefessel L, et al. MicroRNA‐26a is a novel regulator of vascular smooth muscle cell function. J Cell Physiol 2011; 226(4): 1035-43.
17. Sun J, Yan P, Chen Y, Chen Y, Yang J, Xu G, et al. MicroRNA-26b inhibits cell proliferation and cytokine secretion in human RASF cells via the Wnt/GSK-3β/β-catenin pathway. Diagn Pathol 2015; 10(1): 1-9.
18. Dill H, Linder B, Fehr A, Fischer U. Intronic miR-26b controls neuronal differentiation by repressing its host transcript, ctdsp2. Genes Dev 2012; 26(1): 25-30.
19. Icli B, Wara A, Moslehi J, Sun X, Plovie E, Cahill M, et al. MicroRNA-26a regulates pathological and physiological angiogenesis by targeting BMP/SMAD1 signaling. Circ Res 2013; 113(11): 1231-41.
20. Luo X, Pan Z, Shan H, Xiao J, Sun X, Wang N, et al. MicroRNA-26 governs profibrillatory inward-rectifier potassium current changes in atrial fibrillation. J Clin Invest 2013; 123(5): 1939-51.
21. Wang D, Liu C, Wang Y, Wang W, Wang K, Wu X, et al. Impact of miR‐26b on cardiomyocyte differentiation in P19 cells through regulating canonical/non‐canonical Wnt signalling. Cell Prolif 2017; 50(6): e12371.
22. Xu W, Zhang X, Qian H, Zhu W, Sun X, Hu J, et al. Mesenchymal stern cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med (Maywood) 2004; 229(7): 623-31.
23. Tirosh-Finkel L, Zeisel A, Brodt-Ivenshitz M, Shamai A, Yao Z, Seger R, et al. BMP-mediated inhibition of FGF signaling promotes cardiomyocyte differentiation of anterior heart field progenitors. Development 2010; 137(18): 2989-3000.
24. Rowton M, Guzzetta A, Rydeen AB, Moskowitz IP. Control of cardiomyocyte differentiation timing by intercellular signaling pathways. Semin Cell Dev Biol 2021; 118: 94-106.
25. Guo Y, Pu WT. Cardiomyocyte maturation: new phase in development. Circulation research. 2020; 126(8): 1086-106.
26. Buikema JW, Zwetsloot P-PM, Doevendans PA, Domian IJ, Sluijter JP. Wnt/β-catenin signaling during cardiac development and repair. J Cardiovasc Dev Dis 2014; 1(1): 98-110.
27. Mu F, Huang J, Xing T, Jing Y, Cui T, Guo Y, et al. The Wnt/β-catenin/Lef1 pathway promotes cell proliferation at least in part through direct upregulation of miR-17-92 cluster. Front Genet 2019; 10: 525.
28. Marvin MJ, Di Rocco G, Gardiner A, Bush SM, Lassar AB. Inhibition of Wnt activity induces heart formation from posterior mesoderm. Genes Dev 2001; 15(3): 316-27.
29. Hiroi Y, Kudoh S, Monzen K, Ikeda Y, Yazaki Y, Nagai R, et al. Tbx5 associates with Nkx2-5 and synergistically promotes cardiomyocyte differentiation. Nat Genet 2001; 28(3): 276-80.
30. Snyder M, Huang XY, Zhang JJ. Stat3 directly controls the expression of Tbx5, Nkx2. 5, and GATA4 and is essential for cardiomyocyte differentiation of P19CL6 cells. J Biol Chem 2010; 285(31): 23639-46.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadat S, Alani B, Noureddini M, Maleki B, Farrokhian A, Verdi J et al . Evaluation of miR 26b 5p changes during differentiation of human endometrial stem cells into cardiomyocytes. Feyz 2022; 26 (2) :118-127
URL: http://feyz.kaums.ac.ir/article-1-4553-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 26, Issue 2 (Bimonthly 2022) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645