[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 24, Issue 5 (Bimonthly 2020) ::
Feyz 2020, 24(5): 499-507 Back to browse issues page
Study of cell proliferation and migration inhibition by miR-4516 treatment in human breast cancer
Roya Aliasghari-Renani , Zahra Doosti , Somayeh Reiisi
Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, I.R. Iran. , s.reiisi@yahoo.com
Abstract:   (2286 Views)
Background: Breast cancer is the most common cancer among women. Studies have displayed that the dysregulation of microRNAs (miRNAs) plays a crucial role in the incidence, progress, and metastasis of tumors. miRNAs have been considered as a biomarker as well as a therapeutic agent, thus gene therapy with miRNA is considered as one of the ways to treat cancer. miR-4516 has been reported to play a role in different diseases and malignancies. However, the expression level and biological function of miR-4516 in breast cancer remain poorly understood. This study aimed to investigate the expression level and the effect of miR-4516 on the proliferation and migration in breast cancer.
Material and Methods: Tumor and adjacent non-tumor tissues were collected from 40 patients with breast cancer. MiR-4516 levels in breast cancer cell lines and tumor tissue were measured by real-time PCR. Pre-mir-4516 was cloned in the pEGFPN1 vector. Then human breast cancer MCF7 cells were cultured and pre-miR-4516 vector and control vector were transfected into cells. After transfection, a miR-4516 level was verified by QPCR. Cell proliferation and migration were studied with MTT assay and scratch test respectively.
Results: miR-4516 was down-regulated in breast cancer tissues and cell lines. Low expression of miR-4516 was directly related to the high-grade tumor. miR-4516 suppressed breast cancer cell proliferation, but not migration in vitro.
Conclusions: miR-4516 may function as a tumor suppressor and inhibits the proliferation which may consequently serve as a therapeutic target for breast cancer.
Keywords: miR-4516, Breast cancer, Proliferation, Migration
Full-Text [PDF 807 kb]   (1647 Downloads)    
Type of Study: Research | Subject: General
Received: 2020/01/6 | Revised: 2020/12/28 | Accepted: 2020/10/19 | Published: 2020/12/15
References
1. Matamala N, Vargas MT, González-Cámpora R, Miñambres R, Arias JI, Menéndez P, et al. Tumor microRNA expression profiling identifies circulating microRNAs for early breast cancer detection. Clin Chem 2015; 61: 1098-106.
2. Hamam R, Hamam D, Alsaleh KA, Kassem M, Zaher W, Alfayez M, et al. Circulating microRNAs in breast cancer: novel diagnostic and prognostic biomarkers. Cell Death Dis 2017; 8: e3045.
3. Mattick JS, Makunin IV. Small regulatory RNAs in mammals. Hum Mol Genet 2005; 14: R121-R32.
4. Li Y,Kowdley KV. MicroRNAs in common human diseases. Genomics Proteomics Bioinformatics 2012; 10: 246-53.
5. Qin H, Liu W. MicroRNA‐99a‐5p suppresses breast cancer progression and cell‐cycle pathway through downregulating CDC25A. J Cell Physiol 2019; 234: 3526-37.
6. Lin S, Gregory RI. MicroRNA biogenesis pathways in cancer. Nat Rev Cancer 2015; 15: 321.
7. Wahid F, Shehzad A, Khan T, Kim YY. MicroRNAs: synthesis, mechanism, function, and recent clinical trials. Biochimica et Biophysica Acta (BBA)-Molecular Cell Res 2010; 1803(11): 1231-43.
8. Lee Y, Ahn C, Han J, Choi H, Kim J, Yim J, et al. The nuclear RNase III Drosha initiates microRNA processing. Nat 2003; 425: 415.
9. Lund E, Güttinger S, Calado A, Dahlberg JE, Kutay U. Nuclear export of microRNA precursors. Sci 2004; 303: 95-8.
10. Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet 2008; 9: 102.
11. Ardekani AM, Naeini MM. The role of microRNAs in human diseases. Avicenna J Med Biotechnol 2010; 2: 161.
12. Jima DD, Zhang J, Jacobs C, Richards KL, Dunphy CH, Choi WW, et al. Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. Blood 2010; 116: e118-e27.
13. Yoshikawa M, Fujii YR. Human ribosomal RNA-derived resident microRNAs as the transmitter of information upon the cytoplasmic cancer stress. Biomed Res Int 2016; 2016.
14. Chowdhari S, Sardana K, Saini N. miR-4516, a microRNA downregulated in psoriasis inhibits keratinocyte motility by targeting fibronectin/integrin α9 signaling. Biochim Biophys Acta Mol Basis Dis 2017; 1863: 3142-52.
15. Borrelli N, Denaro M, Ugolini C, Poma AM, Miccoli M, Vitti P, et al. miRNA expression profiling of ‘noninvasive follicular thyroid neoplasms with papillary-like nuclear features’ compared with adenomas and infiltrative follicular variants of papillary thyroid carcinomas. Mod Pathol 2017; 30: 39.
16. Bell EH, Kirste S, Fleming JL, Stegmaier P, Drendel V, Mo X, et al. A novel miRNA-based predictive model for biochemical failure following post-prostatectomy salvage radiation therapy. PloS One 2015; 10: e0118745.
17. Asahchop EL, Akinwumi SM, Branton WG, Fujiwara E, Gill MJ, Power C. Plasma microRNA profiling predicts HIV-associated neurocognitive disorder. Aids 2016; 30: 2021-31.
18. Mandourah AY, Ranganath L, Barraclough R, Vinjamuri S, Hof RVT, Hamill S, et al. Circulating microRNAs as potential diagnostic biomarkers for osteoporosis. Sci Rep 2018; 8: 8421.
19. Chowdhari S, Saini N. hsa‐miR‐4516 mediated downregulation of STAT3/CDK6/UBE2N plays a role in PUVA induced apoptosis in keratinocytes. J Cell Physiol 2014; 229: 1630-8.
20. Hu Y, Song J, Liu L, Zhang Y, Wang L, Li Q. microRNA-4516 Contributes to Different Functions of Epithelial Permeability Barrier by Targeting Poliovirus Receptor Related Protein 1 in Enterovirus 71 and Coxsackievirus A16 Infections. Front Cell Infect Microbiol 2018; 8: 110.
21. Samanta D, Almo SC. Nectin family of cell-adhesion molecules: structural and molecular aspects of function and specificity. Cell Mol Life Sci 2015; 72: 645-58.
22. Cui T, Gray A, Liu Z, Geurts M, Robe P, McElroy J, et al. A novel tumor-promoting role for miR-4516 in glioblastoma. AACR 2017.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Aliasghari-Renani R, Doosti Z, Reiisi S. Study of cell proliferation and migration inhibition by miR-4516 treatment in human breast cancer. Feyz 2020; 24 (5) :499-507
URL: http://feyz.kaums.ac.ir/article-1-4063-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 24, Issue 5 (Bimonthly 2020) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645