[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 24, Issue 5 (Bimonthly 2020) ::
Feyz 2020, 24(5): 516-524 Back to browse issues page
Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria
Yaser Jamshidi-Moghaddam , Ahmad Rajabizadeh * , Payam Khazaeli
Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, I.R. Iran. , yaser.jm1366@yahoo.com
Abstract:   (690 Views)
Background: The general tendency of the community to use herbal medicines is due to prove the destructive effects of chemical drugs and environmental pollution. This study aimed to investigate the Anti-bacterial properties of P. gnaphalodes extract on some gram-positive and negative bacteria.
Materials and Methods: In this study, all of the organs of the P. gnaphalodes plant was dried in the shade in the summer of 2016 after collection. The Anti-bacterial effect of the plant was measured on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus to obtain the best extraction method. Next, 15 ml of Muller-Hinton-Agar medium was mixed with concentrations of 10 - 5.2 - 0.25 - 0.625 and 20 mg/ml of the extracts and 104 CFU /ml of the bacterium was inoculated and cultured after growing the colonies. In order to determine the MIC and MBC, the same volumes of 105 CFU/ ml were inoculated into Muller Hinton broth medium containing different concentrations of herbicide extracts. The culture medium was incubated for 24 hours at 37 ° C.
Results: Concentration of 20 mg/ml had the most effect on inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus bacteria, which was eliminated for 90, 68, 75 and 90% bacteria, respectively. MIC and MBC of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus were obtained at 25, 50, 50, 70, 50, 80 and 30 and 50 mg/ml, respectively.
Conclusion: The results showed that the extract of P. gnaphalodes has anti-bacterial effects on some gram positive and negative bacteria.
Keywords: P. gnaphalodes, Plant extracts, Anti-bacterial agents, Gram-positive bacteria, Gram-negative bacteria
Full-Text [PDF 833 kb]   (128 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2019/12/10 | Accepted: 2020/10/12 | Published: 2020/12/15
References
1. Shariatifar N. Quantitative and qualitative study of phenolic compounds and antioxidant activity of plant pulicaria gnaphalodes. HMS 2012; 17(4): 35-41. [in Persian]
2. Calixto BJ. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). BJMBR 2000; 33: 2.
3. Bagheri A, Naghdi BH, Maki ZM, Hemati A, Movahedian F. Evaluation of using herbal medicine in Isfahan women population. JMP 2005; 15: 4
4. Vajargah Sk, motamedi M, vajargah Fk. Study the effect of three medicinal herb kinds as Thyme, Fennel,and Spearmint in health and cure of animal and birds. BAU 2009. [in Persian]
5. ATEŞ DA, ERDOĞRUL ÖT. Antimicrobial activities of various medicinal and commercial plant extracts. TJB 2003; 27(3): 157-62.
6. Hojjati M, and Barzegar H. Chemical composition and biological activities of lemon (Citrus limon) leaf essential oil. NFSR 2017; 4(4): 15-24. [in Persian]
7. Ali MS, Saleem M. Chemical constituents of Pulicaria gnaphalodes. NPS 1999; 5(3): 134-7.
8. Mozafarian V. Lexicon of Iranian plant names. PCV Tehran Press, Tehran; 1996.
9. Shariatifar N. Qualitative and quantitative study of Pulicaria gnaphalodes essential oil and plant extract and evaluation of oxidative stability of soya bean oil in the presence of plant essential oil and extracts: Thesis] TU 2011. [In Persian]
10. Behdani M, Ghazvini K, Mohammadzadeh AR, Sadeghian A. Antibacterial activity of Henna extracts against Staphylococcus aureus and Pseudomonas aeroginosa. HMS 2009; 15(2): 46-51. [in Persian]
11. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. FC 2007; 18(5): 414-20.
12. Daneshvar N, Aber S, Dorraji MS, Khataee A, Rasoulifard M. Photocatalytic degradation of the insecticide diazinon in the presence of prepared nanocrystalline ZnO powders under irradiation of UV-C light. SAPT 2007; 58(1): 91-8 [in Persian]
13. Salehi M, Raeesniya N, Mehrabiyan S. Evaluation of the bacterial effect of external methanolic extracts Pistacia vera. Journal of Microbiological Biotechnology. IAU 2011; 3(10): 53-9. [in Persian]
14. Malakootian M, Toolabi A. Determining and comparing the effect of nanoparticle cuo, tio and zno in removing gram positive and negative bacteria from wastewater. JRHY 2010; 29(2). [in Persian]
15. Molana Z, Shahande Z, Ahmadi MH. The effect of temperature on bacterial resistance Staphylococcus and E. coli to antibiotics. JBUMS 2006; 8(4): 26-31. [in Persian]
16. Moattar F, Asgari M. The Antibacterial Activity of Pulycaria gnaphalodes. JDPS 1990; 1(3): 135-43 . [in Persian]
17. Hui L, He L, Huan L, XiaoLan L, AiGuo Z. Chemical composition of lavender essential oil and its antioxidant activity and inhibition against rhinitis-related bacteria. AJMR 2010; 4(4): 309-13.
18. Hamedi H, Rahimikia E, Gandomi H, Abbaszadeh S, Shariatifar N. chemical composition and antibacterial effect of essential oil from pulicaria gnaphalodes. Turkey Association of Food Technology International Congress on FTTAU 2014. [in Persian]
19. Gandomi H, Abbaszadeh S, Rahimikia E, Shariatifar N. Volatile Organic Compound from Pulicaria gnaphalodes and the Antibacterial and Antifungal Properties of Its Essential Oil and Aqueous, Ethanolic and Methanolic Extracts. JFPP 2015; 39(6): 2129-34. [in Persian]
20. Ebadi A, Monadi A, Pashazadeh M, Zakhireh S. Antibacterial effects alcholic extracts of (Astragalus hamosus) on gram positive and gram negative bacteria. JCPI 2014; 1071-6. [in Persian]
21. Behdani M, Ghazvini K, Mohammadzadeh A, Sadeghian A. Antibacterial activity of Henna extracts against Staphylococcus aureus and Pseudomonas aeroginosa. THMS 2009; 15(2): 46-51. [in Persian]
22. Talei GR, Meshkat AM, Delfan B. Antibacterial activity of fruit, leaves extracts of Artemisia persica boiss, rhus coriaria, ephedra intermedia and daphne mucronata royle of lorestan. UASSH 2004; 19-23. [in Persian]
23. Kapourchali SS, Aliabadi AA, Ghasemi A, Sadeghi SE. Comparing the antibacterial effects of Iranian oak galls extract on some Gram-positive and Gram-negative plant pathogenic bacteria. BPP 2013; 271-40. [in Persian]
24. Azizi Tabrizzad N, Seyedin Ardebili M, Hojjati M. Investigation of chemical compounds and antibacterial activity of pennyroyal, mint and thyme essential oils. FST 2019; 15(85): 447-57. [in Persian]
25. Vajargah SK, Motamedi M, Vajargah FK. Study the effect of three medicinal herb kinds as Thyme, Fennel and Spearmint in health and cure of animal and birds. BAU 2009. [in Persian]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jamshidi-Moghaddam Y, Rajabizadeh A, Khazaeli P. Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria. Feyz. 2020; 24 (5) :516-524
URL: http://feyz.kaums.ac.ir/article-1-4041-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 5 (Bimonthly 2020) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4331