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:: Volume 24, Issue 1 (Bimonthly 2020) ::
Feyz 2020, 24(1): 21-30 Back to browse issues page
Comparison of the apoptotic effects of TPSF small molecule and hydroalcoholic extract of Ziziphus spina-christi on cervical cancer cells
Kiavash Hoshmndi , Elham Hoveizi , Saad Gooraninejad , Mohammadreza Tabandeh
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, I.R. Iran. , e.hoveizi@yahoo.com
Abstract:   (2028 Views)
Background: Cervical cancer is the third most common cancer in the world. Considering the prevalence of cervical cancer in Iran and unsuccessful treatments, the apoptotic effects of TPSF small molecule investigated in comparison with the hydroelectric extract of Ziziphus Spina-christi leaves on the cervical cancer cell line (HeLa).
Materials and Methods: HeLa cells were cultured in a DMEM medium containing 10% FBS, and treated with different concentrations of TPSF and extract on days 1, 3 and 5. Apoptotic effects were investigated using MTT method, and the morphology of cells was studied by Giemsa staining using invert microscope.
Results: 2 μm concentration of TPSF was determined in 24 hours as IC50 for HeLa cells. The percentage of viability in cells treated with 0.5, 1, 2, 5, and 10 μM TPSF was 78, 63, 50, 42 and 37%. The concentration of 4 mM hydroalcoholic extract was determined as IC50 of HeLa cells in 24 hours. The percentage of viability in treated cells with 1, 2, 4, 6 and 8 mM from the extract of was 73, 61, 50, 41, and 37. The results showed a significant (P≤0.05) decrease compared with the control samples. Also, morphological results showed that the treated cells indicated notable changes such as the shrunk nucleus, damaged membrane, and wrinkled cytoplasm compared with the control sample.
Conclusion: The TPSF is significantly better than the extract of Ziziphus Spina-christi, it can be concluded that small molecules have the smallest amount of cytotoxic effect on HeLa cells and have a greater apoptotic effect.
Keywords: Cervical cancer, Ziziphus Spina-christi leave, TPSF, Small molecule
Full-Text [PDF 489 kb]   (828 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/03/1 | Revised: 2020/05/16 | Accepted: 2019/09/29 | Published: 2020/05/16
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Hoshmndi K, Hoveizi E, Gooraninejad S, Tabandeh M. Comparison of the apoptotic effects of TPSF small molecule and hydroalcoholic extract of Ziziphus spina-christi on cervical cancer cells. Feyz 2020; 24 (1) :21-30
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Volume 24, Issue 1 (Bimonthly 2020) Back to browse issues page
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