[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 22, Issue 6 (Bimonthly 2018) ::
Feyz 2018, 22(6): 617-623 Back to browse issues page
Investigating the association of Val/Met polymorphism of the BDNF gene with the incidence of disease in patients with Alzheimer and comparison with healthy elderly people in Iran
Fatemeh Mirzaii-Fini , Mohammad Ali Dowlati * , Mahmood Dehghani Ashkezari , Ebrahim Kouchaki
Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, I. R. Iran. , dr_dovlati@yahoo.com
Abstract:   (1730 Views)
Background: Alzheimerchr('39')s disease is the most common cause of dementia in the elderly and the genetic and environmental factors interfere with its creation. The BDNF gene is responsible for producing a brain-derived neuronal factor. In this disease, the valine66methionine polymorphism and nucleotide changes of 196 (G>A) BDNF are genetic risk factors .This polymorphism has not been investigated in patients with Alzheimerchr('39')s disease in Iran. This study aimed to provide appropriate information on the prognosis of the disease and the ability to get it.
Materials and Methods: In this case-control study, 73 patients with Alzheimerchr('39')s disease and 100 healthy controls were evaluated. Blood samples were taken from the subjects and DNA was extracted. After quantitative and qualitative DNA analysis, PCR-RFLP was performed and the results of both groups were analyzed and compared.
Results: Fourteen patients and seven controls had polymorphisms of BDNF gene. Fifty-nine patients had normal allele, 8 patients with heterozygote allele and 6 patients had methionine/methionine allele. In the controls, 93 patients had normal allele, 5 with heterozygote allele and 2 had methionine/methionine allele.
Conclusion: The findings of this study indicate that the increase in valine/methionine polymorphism in the BDNF gene in Alzheimerchr('39')s patients compared to the control group can express the role of this polymorphism in this disease. Also, patients with this polymorphism had a worse clinical status than patients without this polymorphism. Therefore, evaluation of this polymorphism can provide appropriate information about the patientchr('39')s condition.
Keywords: Alzheimer's disease, BDNF gene, Polymorphism, Valine/methionine
Full-Text [PDF 352 kb]   (536 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/08/21 | Accepted: 2018/10/17 | Published: 2019/01/30
1. Berwick DM. From the Centers for Disease Control and Prevention. Public health and aging: trends in aging-United States and worldwide. JAMA 2003; 289(11): 1371-73.
2. Sabayan B, Bonneux L. Dementia in Iran: how soon it becomes late. Arch Iran Med 2011; 14(4): 290-1.
3. Genecard human database of BDNF. Aveilable at: www.Genecards.org.
4. Mikko Hiltunen, Amolecular genetic study of factors involved in Alzheimer's disease, in Depa‌r‌t‌ment of Clinical Genetics, Chromosome and DNA Laboratory Kuopio University Hospital. Kuopio University: Kuopio; 2001. p. 1-63.
5. Ji H, Dai D, Wang Y, Jiang D, Zhou X, Lin P, et al. Association of BDNF and BCHE with Alzh‌eimer's disease: Meta-analysis based on 56 genetic case-control studies of 12,563 cases and 12,622 controls. Exp Ther Med 2015; 9(5): 1831-40.
6. Cohen-Cory S, Kidane AH, Shirkey NJ, Mar‌shak S. Brain-derived neurotrophic factor and the development of structural neuronal connectivity. Dev Neurobiol 2010; 70(5): 271-88.
7. Ikegame T, Bundo M, Murata Y, Kasai K, Kato T, Iwamoto K. DNA methylation of the BDNF gene and its relevance to psychiatric disorders. J Hum Genet 2013; 58(7): 434-8.
8. Egan MF, Kojima M, Callicott JH, Goldberg TE, Kolachana BS, Bertolino A, et al. The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function. Cell 2003; 112(2): 257-69.
9. American Psychiatric Association: Diagnostic and statistical. manual of mental disorders. 4th ed. 1995.
10. McKhann G, Drachman D, Folstein M, Kat‌z‌man R, Price D, Stadlan EM. Clinical diagnosis of Alzheimer's disease: report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer's Disease. Neurology 1984; 34(7): 939-44.
11. Suriyaprom K, Tungtrongchitr R, Thawn‌a‌som K. Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome. Diabetol Metab Syndr 2014; 6(1): 6.
12. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. Aveilable at: https://blast.ncbi.nlm.nih.gov/Blast. cgi.Blast. 2017.
13. Tessarollo L. Pleiotropic functions of neuro‌trophins in development. Cytokine Growth Factor Rev1998; 9(2): 125-37.
14. Huang EJ, Reichardt LF. Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci 2001; 24: 677-736.
15. Ventriglia M, Bocchio Chiavetto L, Benussi L, Binetti G, Zanetti O, Riva MA, et al. Asso‌ciation between the BDNF 196 A/G polymorphism and sporadic Alzheimer's disease. Mol Psychiatry 2002; 7(2): 136-7.
16. Momose Y, Murata M, Kobayashi K, Ta‌ch‌i‌kawa M, Nakabayashi Y, Kanazawa I, et al. Asso‌ciation studies of multiple candidate genes for Parkinson's disease using single nucleotide polymorphisms. Ann Neurol 2002; 51(1): 133-6.
17. Bian JT, Zhang JW, Zhang ZX, Zhao HL. Association analysis of brain-derived neurotrophic factor (BDNF) gene 196 A/G polymorphism with Alzheimer's disease (AD) in mainland Chinese. Neurosci Lett 2005; 387(1): 11-6.
18. Nagata T, Shinagawa S, Nukariya K, Yamada H, Nakayama K. Association between BDNF poly‌morphism (Val66Met) and executive function in patients with amnestic mild cognitive impairment or mild Alzheimer disease. Dement Geriatr Cogn Disord 2012; 33(4): 266-72.
19. Weinstein G, Beiser AS, Choi SH, Preis SR, Chen TC, Vorgas D, Au R, Pikula A, Wolf PA1, DeStefano AL, Vasan RS, Seshadri S. Serum brain-derived neurotrophic factor and the risk for dementia: the Framingham Heart Study. JAMA Neurol 2014; 71(1): 55-61.
20. Ward DD, Summers MJ, Saunders NL, Jan‌ssen P, Stuart KE, Vickers JC. APOE and BDNF Val66Met polymorphisms combine to influence episodic memory function in older adults. Behav Brain Res 2014; 271: 309-15.
21. Ye Q, Bai F, Zhang Z. Shared Genetic Risk Factors for Late-Life Depression and Alzheimer's Disease. J Alzheimers Dis 2016; 52(1): 1-155.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzaii-Fini F, Dowlati M A, Dehghani Ashkezari M, Kouchaki E. Investigating the association of Val/Met polymorphism of the BDNF gene with the incidence of disease in patients with Alzheimer and comparison with healthy elderly people in Iran. Feyz. 2018; 22 (6) :617-623
URL: http://feyz.kaums.ac.ir/article-1-3672-en.html

Volume 22, Issue 6 (Bimonthly 2018) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4256