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:: Volume 22, Issue 6 (Bimonthly 2018) ::
Feyz 2018, 22(6): 589-594 Back to browse issues page
Cytotoxic effects of Zn oxide nanoparticles against breast cancer T47D cells and NM23 gene expression
Fatemeh Ghodratpour , Fahimeh Baghbani-Arani * , Seyed Ataolah Sadat Shandiz
Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, I. R. Iran. , baghbani.f@gmail.com
Abstract:   (1261 Views)
Background: Breast cancer is the second leading cause of cancer-related death worldwide among women. Nowadays, nanoparticles are interested as anti-cancer factors in studies related to cancer therapy. Therefore, the present study aimed to explore cytotoxic effects of zinc oxide nanoparticles (ZnONPs) and to evaluate NM23 gene expression in T47D breast cancer cells.
Materials and Methods: The cancerous T47D and normal HEK293 cell lines were treated with different concentration of ZnONPs for 24 hours and the cytotoxicity activity of ZnONPs was analyzed by the MTT assay. Then, the NM23 gene expression was evaluated using the real-time PCR method.
Results: According to MTT results, the ZnONPs significantly decreased the viability of T47D cells in dose-dependent manner. Also, the results revealed that the mRNA level of NM23 was up-regulated (7.8-fold) in cells treated with ZnONPs.
Conclusion: ZnONPs can destroy cancerous T47D cells compared to normal HEK293 cells. Furthermore, it seems that ZnONPs can modulate metastasis by enhancing the NM23 gene expression level in breast cancer T47D cells. Thus, ZnONPs can be considered as a promising strategy for the treatment of breast cancer.
Keywords: Breast cancer, ZnONPs, T47D, NM23
Full-Text [PDF 295 kb]   (662 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/08/11 | Accepted: 2018/12/11 | Published: 2019/01/30
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Ghodratpour F, Baghbani-Arani F, Sadat Shandiz S A. Cytotoxic effects of Zn oxide nanoparticles against breast cancer T47D cells and NM23 gene expression. Feyz. 2018; 22 (6) :589-594
URL: http://feyz.kaums.ac.ir/article-1-3663-en.html

Volume 22, Issue 6 (Bimonthly 2018) Back to browse issues page
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