[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 22, Issue 6 (Bimonthly 2018) ::
Feyz 2018, 22(6): 624-631 Back to browse issues page
The study of expression of PTEN and AKT1 genes in co-culturing of HT29 colon cancer cell line with Streptococcus thermophilus
ُShiva Sattari , Changiz Ahmadizadeh
Department of Microbiology, Faculty of Basic Sciences, Ahar Branch, Islamic Azad University, Ahar, I. R. Iran. , dr_ahmadizadeh@yahoo.com
Abstract:   (3216 Views)
Background: Colon cancer is one of the most common cancers in the world. Probiotics are viable and useful microorganisms that have an effective role in controlling cancer by influencing the digestive enzymes of animals and humans, inhibition of cancerous agents in the body and in vitro conditions, suppression of lotions, and cancer-inducing compounds and tumors in experimental animals. The present study was conducted to investigate the PTEN/ AKT1 cellular signaling pathway in adjacent cultures of Streptococcus thermophilus with HT29 colon cancer cells.
Materials and Methods: Bacterial culture, supernatant and bacterial extract were prepared and the cells were treated with these materials. Cell necrosis was evaluated by the MTT method. Also, the expression of PTEN and AKT1 genes in HT29 cell line was investigated using the real-time PCR.
Results: The results showed that the thermophiles bacterium reduced the expression of AKT1 genes, and increased the expression of PTEN and led the cancerous cells toward apoptosis. The MTT test showed that the concentration of OD=0.05 had the highest mortality in 4 hours.
Conclusion: Thermophiles bacteria can be used to create a novel therapeutic effect with high impact, low side effects, harmless biological effect and lower costs. Also, it can be used as an extra treatment appropriate to the body's natural flora for the treatment and prevention of colon cancer.
Keywords: Probiotic, Colon cancer, HT29 cell
Full-Text [PDF 345 kb]   (2247 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/07/22 | Revised: 2019/02/23 | Accepted: 2018/11/21 | Published: 2019/01/30
References
1. Kich DM, Vincenzi A, Majolo F, Volken de Souza CF, Goettert MI. Probiotic: nutrition in cancer treatment and prevention. Nutr Hosp 2016; 29; 33(6): 1430-7.
2. Guandalini S, Cernat E, Moscoso D. Prebiotics and probiotics in irritable bowel syndrome and inflammatory bowel disease in children. Benef Micro bes 2015; 6(2): 209-17.
3. Whelan K. Probiotics and prebiotics in the management of irritable bowel syndrome: a review of recent clinical trials and systematic reviews. Curr Opin Clin Nutr Metab Care 2011; 14(6): 581-7.
4. Barrons R, Tassone D. Use of Lactobacillus probio tics for bacterial genitourinary infections in women: a review. Clin Ther 2008; 30(3): 453-68.
5. Gertler R, Rosenberg R, Schuster T, Friess H. Defining a high-risk subgroup with colon cancer stages I and II for possible adjuvant therapy. Eur J Cancer 2009; 45(17): 2992-9.
6. Slattery ML, Curtin K, Anderson K, Ma KN, Edwards S, Leppert M, et al. Associations between dietary intake and Ki-ras mutations in colon tumors: a population-based study. Cancer Res 2000; 60(24): 6935-41.
7. Brink M, Weijenberg MP, de Goeij AF, Scho‌uten LJ, Koedijk FD, Roemen GM, et al. Fat and K-ras mutations in sporadic colorectal cancer in The Netherlands Cohort Study. Carcinogenesis 2004; 25(9): 1619-28.
8. Pretlow TP, Barrow BJ, Ashton WS, O’Riordan MA, Pretlow TG, Jurcisek JA, et al. Aberrant cry‌pts: putative preneoplastic foci in human colonic mucosa. Cancer Res1991; 51(5): 1564-7.
9. Lye HS, Kuan CY, Ewe JA, Fung WY, Liong MT. The improvement of hypertension by probio‌ti‌cs: effect on cholesterol, diabetes, renin, and phyto‌estrogens. Int J Mol Sci 2009; 10(9): 3755-75.
10. Altonsy MO, Andrews SC, Tuohy KM. Diff‌erential induction of apoptosis in human colonic carcinoma cells (Caco-2) by Atopobium, and commensal, probiotic and enteropathogenic bact‌eria: mediation by the mitochondrial pathway. Int J Food Microbiol 2010; 137(2-3): 190-203.
11. Kelland L. The resurgence of platinum-based cancer chemotherapy. Nat Rev Cancer 2007; 7(8): 573-84.
12. Javidnia K, Miri R, Amirghofran Z, Jafari A, Amoozegar Z. Cytotoxicity and antimicrobial asse‌ss‌ment of Euphoria hebecarpa. Iran J Pharm Res 2004; 3(2): 75-82.
13. Roberfroid MB. Perbiotics and synbio‌tics: co‌nc‌epts and nutriionl propertices. Br J Nutr 1998; 80(4): S197-202
14. Le Leu RK, Hu Y, Brown IL, Woodman RJ, Young GP. Synbiotic intervention of Bifido‌bact‌erium lactis and resistant starch protects against colorectalcancer development in rats. Carcinogenesis 2010; 31(2): 246-51.
15. Le Leu RK, Brown IL, Hu Y, Bird AR, Jackson M, Esterman A, et al. A Synbiotic Comb‌ination of Resistant Starch and Bifidobacterium lactis Facilitates Apoptotic Deletion of Carcinogen-Damaged Cells in Rat Colon1. J Nutr 2005; 135(5): 996-1001.
16. Oliver MH, Harrison NK, Bishop JE, Cole PJ, Laurent GJ. A rapid and convenient assay for cou‌nting cells cultured in microwell plates: application for assessment of growth factors. J Cell Sci 1989; 92(Pt 3): 513-8.
17. Enoki T, Yoshida Y, Lally J, Hatta H, Bonen A. Testosterone increases lactate transport, mono‌carboxylate transporter (MCT) 1 and MCT4 in rat skeletal muscle. J Physiol 2006; 577 (Pt 1): 433-43.
18. Peterson SM, Freeman JL. RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis J Vis Exp 2009; (30). pii: 1470.
19. Keniry M, Parsons R. The role of PTEN sign‌aling perturbations in cancer and in targeted therapy. Oncogene. 2008 Sep 18;27(41):5477-85.
20. Baldwin C, Millette M, Oth D, Ruiz MT, Luquet FM, Lacroix M. Probiotic Lactobacillus acidophilus and L. casei mix sensitize colorectal tumoral cells to 5-fluorouracil-induced apoptosis. Nutr Cancer 2010; 62(3): 371-8.
21. Pan X, Chen F, Wu T, Tang H, Zhao Z. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control 2009; 20(6): 598-602.
22. Montalto M, Maggiano N, Ricci R, Curigliano V, Santoro L, Di Nicuolo F, et al. Lactobacillus aci‌d‌ophilus protects tight junctions from aspirin da‌mage in HT-29 cells. Digestion 2004; 69(4): 225-8.s
23. Chan WK, Cheung CC, Law HK, Lau YL, Chan GC. Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function. J Hematol Oncol 2008; 1: 9.
24. Mair C, Plitzner C, Pfaffl MW, Schedle K, Meyer HH, Windisch W. Inulin and probiotics in newly weaned piglets: effects on intestinal morp‌ho‌logy, mRNA expression levels of inflam‌matory ma‌rker genes and haematology. Arch Anim Nutr 2010; 64(4): 304-21
25. Plaza-Diaz J, Gomez-Llorente C, Fontana L, Gil A. Modulation of immunity and inflammatory gene expression in the gut, in inflammatory diseases of the gut and in the liver by probiotics. World J Gastroenterol 2014; 20(42): 15632-49.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sattari ُ, Ahmadizadeh C. The study of expression of PTEN and AKT1 genes in co-culturing of HT29 colon cancer cell line with Streptococcus thermophilus . Feyz 2018; 22 (6) :624-631
URL: http://feyz.kaums.ac.ir/article-1-3648-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 22, Issue 6 (Bimonthly 2018) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645