[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 22, Issue 4 (Bimonthly 2018) ::
Feyz Med Sci J 2018, 22(4): 355-361 Back to browse issues page
Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman
Hamed Ranjbar Zeidabadi , Babak Kheirkhah *
Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, I. R. Iran. , babak.kheirkhah44@gmail.com
Abstract:   (3197 Views)
Background: Cancer is the second common cause of death in developed countries. Viruses are one of the most important environmental factors which increase the risk of developing cancer. The aim of this study was to identify the human papilloma virus (HPV) in patients with the blood cancer.
Materials and Methods: In this case-control study, blood serum samples were collected from 35 patients with Chronic lymphocytic leukemia (CLL) and 25 patients with Acute lymphoblastic leukemi (ALL) from Kerman medical centers. Thirty healthy individuals were considered as a control group. After DNA extraction from the serum samples, the polymerase chain reaction (PCR) was performed to detect HPV.
Results: The specific sequence of HPV was observed in 15 samples (25%) of the patients’ serum. Out of this number, five samples (14.25%) of the 35 patients had CLL and 10 samples (40%) of 25 patients had ALL. Also, no positive sample was found in the serum of the patients of the control group. Out of the 15 patients suffering from HPV, seven (46.62%) and three (19.98%) had HPV16 and HPV18, respectively and five samples (33.4%) were of other types of HPV.
Conclusion: This study showed that the PCR method with specific primers of Papilloma Virus 16 and 18 is a suitable and accurate method for detecting human papillomavirus.

 
Keywords: Cancer, Human papilloma virus, CLL, ALL
Full-Text [PDF 265 kb]   (2633 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/05/9 | Revised: 2018/10/10 | Accepted: 2018/07/17 | Published: 2018/10/6
References
1. Muñoz N, Bosch FX, De Sanjosé S, Herrero R, Castellsagué X, Shah KV, et al. Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med 2003; 348(6): 518-27.
2. World Cancer Report 2014. World Health Orga‌n‌‌ization; 2014. p. Chapter 5.13. ISBN 9283204298.
3. Hutter JJ. Childhood leukemia. Pediatr Rev 2010; 31(6): 234-41.
4. Zinatizadeh MR, Masoumalinejad Z, Parnak F. Prevalence of Mycoplasma hyorhinis contamination in tissues samples from cancer patients: A Brief Rep‌‌ort. Mod Med Lab J 2017; 1(3): 91-5.
5. Insinga RP, Dasbach EJ, Elbasha EH. Epidemiologic natural history and clinical management of Human Papillomavirus (HPV) Disease: a critical and systematic review of the literature in the development of an HPV dynamic transmission model. BMC Infect Dis 2009; 9(1): 119.
6. De Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H. Classification of papillomaviruses. Virol. 2004; 324(1): 17-27.
7. Baliga MS, Dsouza JJ. Amla (Emblica offi‌cinalis Gaertn), a wonder berry in the treatment and prevention of cancer. Eur J Cancer Prev 2011; 20(3): 225-39.
8. Zur Hausen H. Papillomaviruses in human cancers. Proc Assoc Am Physicians 1999; 111(6): 581-7.
9. Capone RB, Pai SI, Koch WM, Gillison ML, Danish HN, Westra WH, et al. Detection and qua‌ntitation of human papillomavirus (HPV) DNA in the sera of patients with HPV-associated head and neck squamous cell carcinoma. Clin Cancer Res 2000; 6(11): 4171-5.
10. Liu VW, Tsang P, Yip A, Ng TY, Wong LC, Ngan HY. Low incidence of HPV DNA in sera of pre‌‌treatment cervical cancer patients. Gynecol Oncol 2001; 82(2): 269-72.
11. Josefsson AM, Magnusson PK, Ylitalo N, Sørensen P, Qwarforth-Tubbin P, Andersen PK, et al. Viral load of human papilloma virus 16 as a dete‌rminant for development of cervical carcinoma in situ: a nested case-control study. Lancet 2000; 355(9222): 2189-93.
12. Ylitalo N, Sørensen P, Josefsson AM, Mag‌nusson PK, Andersen PK, Pontén J, et al. Cons‌istent high viral load of human papillomavirus 16 and risk of cervical carcinoma in situ: a nested case-control study. Lancet 2000; 355(9222): 2194-8.
13. Tseng CJ, Pao CC, Lin JD, Soong YK, Hong JH, Hsueh S. Detection of human papillomavirus types 16 and 18 mRNA in peripheral blood of advanced cervical cancer patients and its association with prognosis. J Clin Oncol 1999; 17(5): 1391.
14. Dong SM, Pai SI, Rha SH, Hildesheim A, Kurman RJ, Schwartz PE, et al. Detection and quantitation of human papillomavirus DNA in the plasma of patients with cervical carcinoma. Cancer Epidemiol Biomarkers Prev 2002; 11(1): 3-6.
15. Tsai HJ, Peng YW, Lin LY, Chou MC, Lee H, Chiou HL. An association between human papi‌llomavirus 16/18 deoxyribonucleic acid in per‌ipheral blood with p16 protein expression in neo‌plastic cervical lesions. Cancer Detect Prev 2005; 29(6): 537-43.
16. Bodaghi S, Wood LV, Roby G, Ryder C, Steinberg SM, Zheng ZM. Could human papillomaviruses be spread through blood?. J Clin Microbiol 2005; 43(11): 5428-34.
17. Rombaldi RL, Serafini EP, Mandelli J, Zimm‌ermann E, Losquiavo KP. Transplacental transm‌ission of human papillomavirus. Virol J 2008; 5(1): 106.
18. Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet 2013; 382(9895): 889-99.
19. Antonsson A, Karanfilovska S, Lindqvist PG, Hansson BG. General acquisition of human papillomavirus infections of skin occurs in early infancy. J Clin Microbiol 2003; 41(6): 2509-14.
20. Chen AC, McMillan NA, Antonsson A. Human papillomavirus type spectrum in normal skin of individuals with or without a history of frequent sun exposure. J Gen Virol 2008; 89(Pt 11): 2891-7.
21. Kay P, Allan B, Denny L, Hoffman M, Willi‌amson AL. Detection of HPV 16 and HPV 18 DNA in the blood of patients with cervical cancer. J Med Virol 2005; 75(3): 435-9.
22. Kedzia H, Gozdzicka-Jozefiak A, Wolna M, Tomczak E. Distribution of human papillomavirus 16 in the blood of women with uterine cervix carcinoma. Eur J Gynaecol Oncol 1992; 13(6): 522-6.
23. Pao CC, Hor JJ, Yang FP, Lin CY, Tseng CJ. Detection of human papillomavirus mRNA and cervical cancer cells in peripheral blood of cervical cancer patients with metastasis. J Clin Oncol 1997; 15(3): 1008-12.
24. Pao CC, Lin SS, Lin CY, Maa JS, Lai CH, Hsieh TS. Identification of human papillomavirus DNA sequences in peripheral blood mononuclear cells. Am J Clin Pathol 1991; 95(4): 540-6.
25. Xi LF, Koutsky LA, Galloway DA, Kiviat NB, Kuypers J, Hughes JP, et al. Genomic variation of human papillomavirus type 16 and risk for high grade cervical intraepithelial neoplasia. J Natl Cancer Inst 1997; 89(11): 796-802.
26. Szostek S, Zawilinska B, Kopec J, Kosz-Vnenchak M. Herpesviruses as possible cofactors in HPV-16-related oncogenesis. Acta Biochim. Pol. 2009; 56(2): 337.
27. Meshkat Z, Soleimanjahi H, Mahmoudi M, Mirshahabi H, Hassan ZM, Ghaffari SR, et al. Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene. Saudi Med J 2007; 28(10): 1511-5.
28. Ho CM, Yang SS, Chien TY, Huang SH, Jeng CJ, Chang SF. Detection and quantitation of human papillomavirus type 16, 18 and 52 DNA in the peripheral blood of cervical cancer patients. Gynecol Oncol 2005; 99(3): 615-21.
29. Oriel JD. Natural history of genital warts. Br J Vener Dis 1971; 47(1): 1-13.
30. Parkin DM. The global health burden of inf‌ection‐associated cancers in the year 2002. Int J Cancer 2006; 118(12): 3030-44.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ranjbar Zeidabadi H, Kheirkhah B. Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman. Feyz Med Sci J 2018; 22 (4) :355-361
URL: http://feyz.kaums.ac.ir/article-1-3600-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 22, Issue 4 (Bimonthly 2018) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.06 seconds with 46 queries by YEKTAWEB 4660