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Investigating the Effect of Biosynthetic Nano Silver on HDAC8 Expression in Lung Cancer A549 Cell Line
Tooba Ranjbar , Nastaran Asghari Moghaddam * , Azadeh Mohammadgholi
, namoghadam@gmail.com
Abstract:   (67 Views)
Background: Currently nanoparticles have encouraged a broad range of investigations in cancer therapy and diagnosis. In this study, cytotoxicity of nano-silver, synthesized by eucalyptus extract, was measured on lung cancer A549 cell line via MTT assay. In addition, HDAC8 expression changes were analyzed in treated cells by mentioned nanoparticles in comparison to untreated cells. This gene is responsible for repression of transcription in cells. It has been shown that its expression increases in lung cancer. The aim of this study was to determine the effect of synthetic nanoparticles on the expression of HDAC8 gene.
Materials and Methods: In current study, green synthesis of silver nanoparticles were done using the extract of Eucalyptus camaldulensis. In order to evaluate the inhibitory concentration (IC50), cultured A549 cells were treated by different concentrations of synthesized nanoparticles and MTT assay was performed. Finally, the HDAC8 expression change of cells treated by inhibitory concentration was analyzed since this gene considered as an oncogenic factor by Real-time PCR.
Results: Results from MTT revealed that the obtained nanoparticles decreased the viability of cells in concentration-dependent manner. The IC50 value of silver nanoparticles against A549 cells was 2 μg/ml. Also, the HDAC8 expression showed 9-fold decrease in treated cells.
Conclusion: According to the results, Nano silver particles produced by eucalyptus extract could cause apoptosis by decrease of HDAC8 expression. Hence, they can be considered as an agent with promising aspects to treat lung cancer.
 
Keywords: silver nanoparticles, eucalyptus extract, lung cancer, cell line A549, HDAC8
     
Type of Study: Research | Subject: General
Received: 2018/02/2 | Accepted: 2018/08/18
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