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:: Volume 21, Issue 4 (Bimonthly 2017) ::
Feyz 2017, 21(4): 335-344 Back to browse issues page
The effect of alcoholic extract of Panicum miliaceum L. seed on hippocampus neuronal density in male mouse
Arezoo Bornarodi, Maryam Tehranypour *, Naser Mahdavi-Shahri
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran. , maryam_tehranipour@mshdiau.ac.ir
Abstract:   (1488 Views)
Background: Hippocampus organization is a part of temporal lobe, which consists of several sections including hippocampal body, dentate gyrus and subiculum. Panicum miliaceum L. contains proteins, vitamins and antioxidants for human health. This study was conducted to examine the effect of the alcoholic extract of the seed of Panicum miliaceum L. plant on hippocampus neuronal density.
Materials and Methods: In this experimental study, 24 male mice were divided into 4 groups (n=6, each group). The alcoholic extract of the seed of the Panicum miliaceum L. plant was prepared by soxhlet extraction. Three doses of the extract 25, 50, 75 mg/kg were intraperitoneally injected to 3 treatment groups for 21 days and the control group received normal saline injection. At the end of the experiment, the animals were anesthetized and after perfusion, their brains were removed from the skull. After tissue processing, slices of the brain were prepared and stained. Then, different regions of the hippocampus were photographed and neuronal densities were evaluated.
Results: Results showed that the neuronal density in the CA1, CA3 regions of the group treated with 50 mg/kg of the alcoholic extract and in all regions of hippocampus (CA1,CA2,CA3) in groups treated with dose of 75 mg/kg of the alcoholic extract had a significant increase compared to the control group (P<0.05).
Conclusion: The present study shows that the alcoholic extract of the seed of Panicum miliaceum L. plant increases neuronal density and induces neurogenesis in the mouse hippocampus.
Keywords: Panicum miliaceum, Alcoholic extract, Neuronal density, Hippocampus
Full-Text [PDF 675 kb]   (425 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/05/1 | Accepted: 2017/08/15 | Published: 2017/10/8
References
1. Gorden M.s. Neurobiology. 3rd ed. Oxford university press; 2000. p. 618-34.
2. Guyton AC, Hall JE. Textbook of Medical physiology. 3rd. Philadelphia: Saunders; 2008. p. 645-643.
3. Jahanshahi M, Sadeghi Y, Hosseini A, Naghdi N, Piriaie A. Working memory learning method and astrocytes number in different subfields of rat’s hippocampus. J Animal Veterinary 2008; 3(1): 31-28.
4. Achille G, Synthia H. Ganadotropins and Progestogens: Obligatory developmental function during early embryogenesis and their role in adult neurogenesis, neuroregeneration and neurodegeneration. Academic Press 2011; 1: 375-305.
5. Taupin P. The hippocampus: neurotransmission and plasticity in the nervous system. 1st. New York: Nova Biomedical Books; 2008. P. 25-20.
6. Baker RD. Millet production. Cooperative Extension Service, New Mexico State 1996; 1: 41-1.
7. Kazemi Arbat H. private farming. Jahad University Press 2003; p. 228-17. [Persian]
8. Hulse JH, Laing EM, Pearson OE. Sorghum and the millets: their composition and nutritive value. New York: Academic Press; 1980. P. 997-1.
9. Kalinova J, Moudry J. Content and quality of protein in proso millet (Panicum miliaceum L.) varieties. Plant Foods Hum Nutr 2006; 9: 61-45.
10. Chandrasekara A, Shahidi F. Content of insoluble bound phenolics in millets and their contribution to antioxidant capacity. J Agric Food Chem 2010; 58: 6706-14.
11. Mehla J, Pahuja M, Gupta YK. Streptozotocin- Induced Sporadic Alzheimer's Disease: Selection of Appropriate Dose. J Alzheimers Dis 2013; 33(1): 21-17.
12. Williams R, Spencer J. Flavonoids. Cognition and dementia: actions, mechanisms and potential therapeutic utility for Alzheimer’s disease. Free Radical Bio Med 2011; 127(3): 213-210.
13. Andres-Lacueva CB, Shukitt-Hale RL, Galli O, Jauregui RM, et al. Anthocyanins in Aged Blueberry-Fed Rats Are Found Centrally and May Enhance Memory. Nutr Neurosci 2005; 8(2): 111-20.
14. Querfurth HW, LaFerla FM. Alzheimer's Disease. N Engl J Med 2010; 362(4): 329-44.
15. Tahmasebi S, Heidarien N, Mohagerani HR. Effects of Crataegus Aronia on Passive Avoidance Learning in Wistar Male Rats. J Cellular Molecular Biotechnol News 2013: 86-79.
16. Choi Y, Jeong H-S, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 2007; 103(1): 130-8.
17. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Scientific World J 2013; 20:750-162.
18. Pradeep P.M, Yadahally N, Sreerma N. Soluble and bound phenolics of two different millet genera and their milled fractions: Comparative evaluation of antioxidant properties and inhibitory effects on starch hydrolysing enzyme activities. J Functional Foods 2017; 35:693-685.
19. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biol Int 2006; 160: 40-1.
20. Truswell AS. Cereal grain and coronary heart disease. Eur J Clin Nutr 2002; 56(1): 4-1.
21. Tehranipour M, Sabzalizade M. Effect of Cannabis sativa alcoholic extract on hippocampus neuronal density in Rats. J Gorgan Univ Med Sci 2011; 13(2): 15-9. [in Persian]
22. Behnam-rasouli M, Nikravesh M, Mahdavi N, Tehranipour M. Post-Operative time effects after sciatic nerve crush on the number of alpha motoneurons, using a stereological counting method (disector). Iran Biomedical J 2000; 4(1): 45-9.
23. Kempermann G, Kuhn H.G, Gage F.H. More hippocampal neurons in adult mice living in an enriched environment. Nature 1997; 386: 495-493.
24. Gage F. Brain repair yourself. Scientific American 2003; 289(3): 95-87.
25. Rendeiro C, Rhodes JS, Spencer JP. The mechanisms of action of flavonoids in the brain: Direct versus indirect effects. Neurochem Int 2015; 89: 139-126.
26. Wang H, Wang H, Cheng H, Che Z. Ameliorating effect of luteolin on memory impairment in an Alzheimer's disease model. Mol Med Rep 2016; 13: 4220–15.
27. Oberbauer E, Urmann C, Steffenhagen C, Bieler L, Brunner D, Furtner T, et al. Chroman-like cyclic prenylflavonoids promote neuronal differentiation and neurite outgrowth and are neuroprotective. J Nutr Biochem 2013; 24: 1962–53.
28. Macready AL, Kennedy OB, Ellis JA, Williams CM, Spencer JP, Butler LT. Flavonoids and cognitive function: a review of human randomized controlled trial studies and recommendations for future studies. Genes Nutr 2009; 4: 227-42.
29. Ashrafpour M, Parsaei S, Sepehri H. Quercetin improved spatial memory dysfunctions in rat model of intracerebroventricular streptozotocin-induced sporadic Alzheimer's disease. Natl J Physiol 2015; 5: 411-5.
30. Vauzour D, Vafeiadou K, Rodriguez-Mateos A, Rendeiro C, Spencer PE. The neuroprotective potential of flavonoids: a multiplicity of effects. Genes Nutr 2008 Dec; 3(3-4): 115-26.
31. Pocernich CB, Lange ML, Sultana R, Butterfield DA. Nutritional approaches to modulate oxidative stress in Alzheimer's disease. Curr Alzheimer Res 2011; 8: 469-52.
32. Lei X, Chao H, Zhang Z, Lv J, Li S, Wei H, et al. Neuroprotective effects of quercetin in a mouse model of brain ischemic/reperfusion injury via anti-apoptotic mechanisms based on the Akt pathway. Mol Med Rep 2015; 12: 3696–88.
33. Abd El, Baky AE. Quercetin protective action on oxidative stress, sorbitol, insulin risistance and β-cells function in expermintal diabetic rats. International J Pharmaceutical Studies Res 2011; 2(2): 7-1.
34. Heo HJ, Lee CY. Protective effects of quercetin and vitamin C against oxidative stress-induced neurodegeneration. J Agric Food Chem 2004; 52(25): 7514-7.
35. Sriraksa N, Wattanathorn J, Muchimapura S, Tiamkao S, Brown K, Chaisiwamongkol K. Cognitive-Enhancing Effect of Quercetin in a Rat Model of Parkinson's Disease Induced by 6- Hydroxydopamine. Evidence- Based Complementary and Alternative Medicine (eCAM). 2012; 212: Article ID 823206.
36. Rattray M. Is there nicotinic modulation of nerve growth factor? Implications for cholinergic therapies in Alzheimer's disease. Biol Psychiatry 2001; 49(3): 185-93.
37. Kosaka K, Yokoi T. Carnosic Acid, a component of Rosemary (Rosmarinusofficinalis L.), promotes synthesis of nerve growth factor in T98G human glioblastoma cells. Biol Pharm Bull 2003; 26: 1622-1620.
38. Rajasekaran NS, Nithya M, Rose C, Chandra TS. The effect of finger millet feeding on the early responses during the process of wound healing in diabetic rats. Biochim Biophys Acta 2004; 1689(3–4): 190-201.
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Bornarodi A, Tehranypour M, Mahdavi-Shahri N. The effect of alcoholic extract of Panicum miliaceum L. seed on hippocampus neuronal density in male mouse. Feyz. 2017; 21 (4) :335-344
URL: http://feyz.kaums.ac.ir/article-1-3327-en.html


Volume 21, Issue 4 (Bimonthly 2017) Back to browse issues page
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