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:: Volume 21, Issue 5 (Bimonthly 2017) ::
Feyz 2017, 21(5): 407-413 Back to browse issues page
The effect of hydroalcoholic Ziziphus spina-christi leaf extract on viability of breast cancer cell line (MCF7) and evaluation of Bax and Bcl2 genes expression level
Rahim Ahmadi , Sasan Rahimi , Nadia Ehteshamzad
Department of Biology, Hamedan Branch, Islamic Azad University, Hamedan, I. R. Iran. , rahahmadi2001@yahoo.com
Abstract:   (4670 Views)
Background: Studies have revealed that the Sidr (Ziziphus spina-christi) leaf has anticancer effects. The aim of this study was to investigate the effects of hydroalcoholic Sidr leaf extract on MCF7 cell line viability and evaluation of Bax and Bcl2 genes expression level.
Materials and Methods: In this laboratory-experimental study, MCF cells were randomly divided into control group and groups exposed to 0.001, 0.01, 0.1, 1 and 10 mg/mL of the Sidr leaf hydroalcoholic extract. The cytotoxic effect of the extract was measured using the MTT assay method. Also, the real-time polymerase chain reaction method was used to evaluate Bax and Bcl2 genes expression levels.  
Results: Viability of the MCF7 cells did not significantly change in group exposed to 0.001 mg/mL of the extract; however, it was significantly decreased in groups exposed to 0.01, 0.1, 1 and 10 mg/mL of the extract (P<0.01, P<0.05, P<0.001 and P<0.001, respectively). The expression levels of Bcl2 and Bax genes were significantly decreased and increased respectively in MCF7 cells exposed to 1mg/mL of the extract (P<0.01 and P<0.001, respectively). 
Conclusion: The appropriate doses of the hydroalcoholic Sidr leaf extract have cytotoxic effects on MCF7 cells by inducing apoptosis. So, further research on the anticancer effects of Sidr on breast cancer has a significant place in breast cancer treatment.
Keywords: Hydroalcoholic extract, Ziziphus spina-christi, MCF7, Viability, BAX, BCL2, Apoptosis
Full-Text [PDF 248 kb]   (2385 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/03/22 | Revised: 2017/12/10 | Accepted: 2017/09/10 | Published: 2017/11/14
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Ahmadi R, Rahimi S, Ehteshamzad N. The effect of hydroalcoholic Ziziphus spina-christi leaf extract on viability of breast cancer cell line (MCF7) and evaluation of Bax and Bcl2 genes expression level. Feyz 2017; 21 (5) :407-413
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Volume 21, Issue 5 (Bimonthly 2017) Back to browse issues page
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