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:: Volume 21, Issue 1 (Bimonthly 2017) ::
Feyz Med Sci J 2017, 21(1): 9-18 Back to browse issues page
Optimizing conditions for the production of antifungal agents using the native Bacillus cereus SB15
Hosein Motamedi * , Elnaz Zahedi , Ali Zarei-Mahmoud Abadi
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, I. R. Iran. , hhmotamedi@yahoo.com
Abstract:   (3584 Views)

Background: With regard to the occurrence of fungal infections, finding new antifungal agents is necessary. Due to its biologic properties Bacillus spp. are suitable sources for this purpose. The aim of this study was to find native bacillus species with potential of antifungal agent production.

Material and Methods: In this experimental study, bacillus species were isolated from heat-treated soil samples and following culturing in TSB, anti-fungal effects of their supernatants were determined by disc diffusion method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) levels of the selected strains were obtained against two fungal pathogens. Growth curve of the isolate was obtained in different pH and temperatures. The effects of pH, temperature, carbon and nitrogen sources on antibiotic production by the isolate were also investigated. This isolate was identified through biochemical and molecular tests.

Results: Three out of six bacillus isolates were able to produce the antifungal agents, among them Bacillus cereus strain SB15 showed the most significant antifungal effects against a wide range of fungi. Optimized conditions for antibiotic production by this isolate were: temperature=37˚C, pH=7, 48 h incubation and manitol and urea as the best carbon and nitrogen sources, respectively. The MIC and MFC of the produced antibiotic against Aspergillus niger were: 2 and 4 and against Aspergillus flavus 4 and 16 mg/ml, respectively.

Conclusion: Based on the obtained results it may be concluded that this bacillus strain has optimum potential for antifungal agent production. The strain can be used for the control of fungal infections and also fungal growth in food industry and environment.

Keywords: Antifungal agents, Bacillus, Antibiotic, Optimization, Antibiotic resistance
Full-Text [PDF 324 kb]   (1627 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/03/6 | Revised: 2017/07/17 | Accepted: 2017/03/6 | Published: 2017/03/6
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Motamedi H, Zahedi E, Zarei-Mahmoud Abadi A. Optimizing conditions for the production of antifungal agents using the native Bacillus cereus SB15. Feyz Med Sci J 2017; 21 (1) :9-18
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Volume 21, Issue 1 (Bimonthly 2017) Back to browse issues page
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