[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 1 (Bimonthly 2017) ::
Feyz 2017, 21(1): 74-82 Back to browse issues page
Determination of antibiotic resistance profile in Klebsiella pneumonia strains isolated from urinary tract infections of patients hospitalized in Peyambaran hospital (Tehran-Iran)
Marzieh Tavakol, Hasan Momtaz *
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord. I. R. Iran. , hamomtaz@yahoo.com
Abstract:   (3501 Views)

Background: Urinary tract infection (UTI) is the second prevalent infection in human  mostly caused by Escherichia coli and Klebsiella pneumonia. The aim of this study was to determine the antibiotic resistance profile and detect the prevalence of antibiotic resistance encoding genes in K .pneumoniae isolated from UTI.

Materials and Methods: Fifty K. pneumonia strains isolated from 122 UTI samples of hospitalized patients in Payambaran Hospital (Tehran, Iran) which were subjected to this study (2014) were confirmed by standard biochemical tests. Isolates were tested for susceptibility to 10 antimicrobial drugs by using disk diffusion method. Antibiotic resistance encoding genes frequently include the aadA1, aac(3)-IV, sul1, blaSHV, Cat1, cmlA, tetA, tetB, dfrA1, CITM, qnr in isolates were determined by  PCR.  

Results: The highest antibiotic resistance in K. pneumoniae isolates were for Tetracycline and the lowest   resistance (2%) for Gentamicin and Imipenem. To determine the frequency of antibiotic resistant genes, 64% and 4% of isolates had tetA and Gentamicin-(aac(3)-IV)  resistant genes, respectively.

Conclusion: Frequency of antibiotic resistance encoding genes may have important and basic role in the occurrence and transfer of antibiotic resistance which can be due to the indiscriminate use of antibiotics.  

Keywords: Urinary tract infection, Klebsiella pneumonia, antibiotic resistance profile
Full-Text [PDF 326 kb]   (1310 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/03/1 | Accepted: 2017/03/1 | Published: 2017/03/1
1. Kim JM, Park ES, Jeong JS, Kim KM, Kim JM, Oh HS, et al. Multicenter surveillance study for nosocomial infections in major hospitals in Korea. Nosocomial Infection Surveillance Committee of the Korean Society for Nosocomial Infection Control. Am J Infect Control 2000; 28(6): 454-8.
2. Larypoor M, Frsad S. Evaluation of nosocomial infections in one of hospitals of Qom, 2008. Iran J Med Microbiol 2011; 5(3): 7-17. [in Persian]
3. Sefton AM. The impact of resistance on the management of urinary tract infections. Int J Antimicrob Agents 2000; 16(4): 489-91.
4. Ahanjan M, Haghshenas MR, Naghshvar F, Bairamvand E. Survey and detect of bacteria caused UTI in patients referring to the Imam Khomeini hospital in Sari city 1388-89. J Mazand Univ Med Sci 2013; 22(Suppl 1): 82-6. [in Persian]
5. Yankowitz J, Niebyl JR. Drug therapy in pregnancy. 3rd ed. Philadelphia: Lippincott Williams & Wilkins; 2001. p. 63-72.
6. DelzellJr JE, Lefevre ML. Urinary tract infections during pregnancy. Am Fam Physician 2000; 61(3): 713-20.
7. Falade AG, Ayede AI. Epidemiology, etiology and management of childhood acute community-acquired pneumonia in developing countries--a review. Afr J Med Med Sci 2011; 40(4): 293-308.
8. Livermore DM. Current epidemiology and growing resistance of gram-negative pathogens. Korean J Intern Med 2012; 27(2): 128-42.
9. Lu PL, Liu YC, Toh HS, Lee YL, Liu YM, Ho CM, et al. Epidemiology and antimicrobial susceptibility profiles of Gram-negative bacteria causing urinary tract infections in the Asia-Pacific region: 2009-2010 results from the Study for Monitoring Antimicrobial Resistance Trends (SMART). Int J Antimicrob Agents 2012; 40 Suppl: S37-43.
10. Gootz TD. The global problem of antibiotic resistance. Crit Rev Immunol 2010; 30(1): 79-93.
11. Jalalpoor SH, KasraKermanshahi R, Nouhi AS, ZarkeshIsfahani H. Comparing the frequency of β-lactamase enzyme in isolated nosocomial infectious bacteria. JRMS 2009; 8(3): 203-14. [in Persian]
12. Boucher Y, Labbate M, Koenig JE, Stokes HW. Integrons: mobilizable platforms that promote genetic diversity in bacteria. Trends Microbiol 2007; 15(7): 301-9.
13. Lapierre L, Cornejo J, Borie C, Toro C, San Martín B. Genetic characterization of antibiotic resistance genes linked to class 1 and class 2 integrons in commensal strains of Escherichia coli isolated from poultry and swine. Microb Drug Resist 2008; 14(4): 265-72.
14. Mahon CR, Lehman DC, Manuselis G. Textbook of diagnostic microbiology. 5th ed. New York: Saunders; 2014.
15. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility testing; 20th informational supplement. CLSI/NCCLS M100-S20. Clinical and Laboratory Standards Institute, Wayne, Pa; 2010.
16. Forbes AB, Sahm DF, Weissfeld AS. Bailey and Scott's Diagnostic Microbiology, 12th ed. Philadelphia. Mosby; 2007. p. 236-40, 365-584.
17. Liu Y, Liu C, Zheng W, Zhang X, Yu J, Gao Q, et al. PCR detection of Klebsiella pneumoniae in infant formula based on 16S–23S internal transcribed spacer. Int J Food Microbiol 2008; 125(3): 230–5.
18. Van TT, Chin J, Chapman T, Tran LT, Coloe PJ. Safety of rawmeat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes. Int J Food Microbiol 2008; 124(3): 217-23.
19. Randall LP, Cooles SW, Osborn MK, Piddock LJ, Woodward MJ. Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-fiveserotypes of Salmonella enterica isolated from humans and animals in the UK. J Antimicrob Chemother 2004; 53(2): 208-16.
20. Toro CS, Farfán M, Contreras I, Flores O, Navarro N, Mora GC, et al. Geneticanalysis of antibiotic-resistance determinants in multidrug-resistant Shigella strains isolated from Chileanchildren. Epidemiol Infect 2005; 133(1): 81-6.
21. Mammeri H, Van De Loo M, Poirel L, Martinez-Martinez L, Nordmann P. Emergence of plasmid-mediated quinolone resistance in Escherichia coli in Europe. Antimicrob Agents Chemother 2005; 49(1): 71-6.
22. Stanton SL, Dwyer PL. Urinary tract infection in female. 1st ed. London: Marlin Duntize; 2000. p. 304.
23. Centers for Disease Control and Prevention (CDC). Perinatal group B streptococcal disease after universal screening recommendations. United States, 2003-2005. MMWR Morb Mortal Wkly Rep 2007; 56(28): 701-5.
24. Rijavec M, StarcicErjavec M, Ambrozic Avgustin J, Reissbrodt R, Fruth A, Krizan-Hergouth V, et al. High prevalence of multidrug resistance and ran dom distribution of mobile genetic elements among uropathogenic Escherichia coli (UPEC) of the four major phylogenetic groups. Curr Microbiol 2006; 53(2): 158–62.
25. Tsering DC, Das S, Adhiakari L, Pal R, Singh TS. Extended Spectrum Beta-lactamase Detection in Gram-negative Bacilli of Nosocomial Origin. J Glob Infect Dis 2009; 1(2): 87-92.
26. Mathai E, Grape M, Kronvall G. Integrons and multidrug resistance among Escherichia coli causing communityacquired urinary tract infection in southern India. APMIS 2004; 112(3): 159–64.
27. Gulsun S, Oguzoglu N, Inan A, Ceran N. The virulence factors and antibiotic sensitivities of Escherichia coli isolated from recurrent urinary tract infections. Saudi Med J 2005; 26(11): 1755–58.
28. Tariq N, Jaffery T, Ayub R, Alam AY, Javid MH, Shafique S. Frequency and antimicrobial susceptibility of aerobic bacterial vaginal isolates. J Coll Physicians Surg Pak 2006; 16(3): 196–99.
29. SoltanDalal MM, Miremadi S, SharifyYazdi M K, RastegarLari A, Rajabi Z, AvadisYans S. Antimicrobial Resistance Trends Of Klebsiella Spp. Isolated From Patients In Imam Khomeini Hospital. J Payavard Salamat 2012; 6(4): 275-81. [in Persian]
30. Amin A, Ghumro PB, Hussain S, Hameed A. Prevalence of antibiotic resistance among clinical isolates of Klebsiella pneumonia isolated from a Tertiary Care Hospital in Pakistan. Malays J Micro 2009; 5(2): 81-6.
31. Ishii Y, Alba J, Kimura S, Shiroto K, Yamaguchi K. Evaluation of antimicrobial activity of Blactam antibiotics using E-test against clinical isolates from 60 medical centers in Japan. J Antimicrob Agent 2005; 25(4): 296-301.
32. Al Shara MA. Emerging Antimicrobial Resistance of Klebsiella pneumonia strains Isolated from Pediatric Patients in Jordan. Iraqi J Med 2011; 7(2): 29-32.
33. Du J, Li P, Liu H, Lü D, Liang H, Dou Y. Phenotypic and molecular characterization of multidrug resistant Klebsiella pneumoniae isolated from a university teaching hospital, China. PloS One 2014; 9(4): e95181.
34. Dormanesh B, Mirnejad R, Khodaverdi Dariyan E, Momtaz H, Yahaghi E, Safarpour Dehkordi F, et al. Characterization and study the antibiotic resistance of Uropathogenic Escherichia coli isolated from pediatrics with pyelonephritis and cystitis in Iran. Iran J Med Microbiol 2013; 7(2): 27-39.
35. Machado E, Coque TM, Canton R, Novais A, Sousa JC, Baquero F, et al. Portuguese Resistance Study Group. High diversity of extended-spectrum betalactamases among clinical isolates of Enterobacteriaceae from Portugal. J Antimicrob Chemother 2007; 60(6): 1370-74.
36. Khorshidi A, Moazen Z, Rohani M, Moniri R, shagari GH. Prevalence of TEM and SHV1 genes in Kelebsiella pneumoniea with ESBL. J Mil Med 2009; 3(11): 153-49. [in Persian]
37. Shams E, Firoozeh F, Moniri R, Zibaei M. Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran. J Pathog 2015; 2015: 434391.
38. Shibl A, Al-Agamy M, Memish Z, Senok A, Khader SA, Assiri A. The emergence of OXA-48- and NDM-1-positive Klebsiella pneumoniae in Riyadh, Saudi Arabia. Int J Infect Dis 2013; 17(12): 1130–3.
39. Teo J, Ngan G, Balm M, Jureen R, Krishnanb P, Lina R. Molecular characterization of NDM-1producing Enterobacteriaceae isolates in Singapore hospitals. Western Pac Surveill Response J 2012; 3(1): 19-24.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tavakol M, Momtaz H. Determination of antibiotic resistance profile in Klebsiella pneumonia strains isolated from urinary tract infections of patients hospitalized in Peyambaran hospital (Tehran-Iran). Feyz. 2017; 21 (1) :74-82
URL: http://feyz.kaums.ac.ir/article-1-3286-en.html

Volume 21, Issue 1 (Bimonthly 2017) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4282