[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 1 (Bimonthly 2017) ::
Feyz 2017, 21(1): 19-27 Back to browse issues page
Effect of aqueous extract of mango kernel (Mangifera Indica) on basal activity of virgin rat uterine smooth muscle: role of muscarinic receptors
Mehdi Noureddini *, Masoumeh Dehghan-Sheibani, Behrang Alani
Physiology Research Center, Kashan University of Medical Sciences, Kashan, I. R. Iran. , mnoureddini@yahoo.com
Abstract:   (1529 Views)

Background: Mango belongs to the Anacardiaceae and the extracts from its stems, leaves, fruit and kernel are reported to affect smooth muscle contractility. We studied the role of   cholinergic muscarinic receptors for the effects of aqueous extract of mango kernel (Mangifera indica) on the basal activity of virgin rat uterine smooth muscle.

Materials and Methods: In this experimental study, mid-sections (n=24) of the uterine of healthy virgin rats were placed in an organ bath containing carbonated Tyrode’s solution under 1 g tension. The cumulative effects of the aqueous extracts of mango kernel (0.002, 0.02, 0.2, 2, 20, 200, and 2000 μg/mL) or extract vehicle (Tyrode’s solution) in the presence or absence of atropine were examined by isometric method using the strength, frequency and contractile activity of uterine smooth muscle.

Results: The cumulative concentrations (0.002-20 µg/ml) of mango kernel aqueous extract was significantly decreased the strength, frequency and contractile activity of uterine smooth muscle, but the contractile activity was returned to the basal level at the concentrations of 200 and 2000 µg/ml. Atropine (1 µM) could not significantly change the effects of cumulative use of extract on the strength and contractile activity of uterine smooth muscle, but it significantly enhanced the contractile frequency at low concentrations.

Conclusion: The effects of aqueous extract of mango kernel on the activity of the uterine smooth muscle might not be through cholinergic muscarinic receptors and atropine could enhance the effects of the extract on frequency through other receptors.

Keywords: Aqueous extract, Mango, Muscarinic receptors, Uterine smooth muscle, Rat
Full-Text [PDF 375 kb]   (499 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/02/26 | Accepted: 2017/02/26 | Published: 2017/02/26
References
1. Chang E. Preterm birth and the role of neuroprotection. BMJ 2015; 350: g6661.
2. Dolan SM. Genetic and environmental contributions to racial disparities in preterm birth. Mt Sinai J Med 2010; 77(2): 160-65.
3. Swaggart KA, Pavlicev M, Muglia LJ.Genomics of Preterm Birth. Cold Spring Harb Perspect Med 2015; 5(2): a023127.
4. Engstrøm T. Myometrial receptors in rat parturition. Dan Med Bull 2003; 50(3): 219-37.
5. López Bernal A. The regulation of uterine relaxation. Semin Cell Dev Biol 2007; 18(3): 340-47.
6. Darios ES, Seitz B, Watts SW. Smooth muscle pharmacology in the isolated virgin and pregnant rat uterus and cervix. J Pharmacol Exp Ther 2012; 341(3): 587-96.
7. Crankshaw DJ. Pharmacological techniques for the in vitro study of the uterus. J Pharmacol Toxicol Methods 2001; 45(2): 123-40.
8. Varol FG, Hadjiconstantinou M, Zuspan FP, Neff NH. Pharmacological characterization of the muscarinic receptors mediating phosphoinositide hydrolysis in rat myometrium. J Pharmacol Exp Ther 1989; 249(1): 11-5.
9. Vogel JP, Nardin JM, Dowswell T, West HM, Oladapo OT. Combination of tocolytic agents for inhibiting preterm labour. Cochrane Database Syst Rev 2014; (7): CD006169.
10. Hösli I, Sperschneider C, Drack G, Zimmermann R, Surbek D, Irion O. Swiss Society of Obstetrics and Gynecology. Tocolysis for preterm labor: expert opinion. Arch Gynecol Obstet 2014; 289(4): 903-9.
11. Cordell GA, Colvard MD. Natural products and traditional medicine: turning on a paradigm. J Nat Prod 2012; 75(3): 514-25.
12. Bielory L, Russin J, Zuckerman GB. Clinical efficacy, mechanisms of action, and adverse effects of complementary and alternative medicine therapies for asthma. Allergy Asthma Proc 2004; 25(5): 283-91.
13. Mills E, Dugoua JJ, Perri D, Koren G. Herbal medicines in pregnancy and lactation: an evidence-based approach: CRC Press; 2013.
14. Ahangarpour A, Heidari R, Abdollahzadeh M, Oroojan AA. Antispasmodic Effects of Aqueous and Hydroalcoholic Punica granatum Flower Extracts on the Uterus of Non-pregnant Rats. J Reprod Infertil 2012; 13(3): 138-42.
15. Noureddini M, Namdar F, Hidari A, Benafshe H. The effect of aqueous extract of pomegranate seed on rat uterine smooth muscle contractility. Physiol Pharmacol 2014; 17(4): 461-8. [in Persian]
16. Habibian R, Rafieian Kopaei M, Mohammadzade Z. Effect of hydroalcoholic extract of kelussia odoratissima mozaffarian on uterus contractions of mature rats. Quarterly Horizon Med Sci 2015; 21(3): 169-74. [in Persian]
17. Eslamian SS, Sharifani M. Humid Region Crops. Aeij Publishing; 2010. P. 145-78. [In Persian]
18. Shah KA, Patel MB, Patel RJ, Parmar PK. Mangifera Indica (mango). Pharmacogn Rev 2010; 4(7): 42-8.
19. Bragança VL, Melnikov P, Zanoni LZ. Trace element in fruit juices. Biol Trace Elem Res 2012; 146(2): 256-61.
20. Arif M, Fareed S, Rahman MA. Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice. J Pharm Bioallied Sci 2016; 8(1): 58-63.
21. Parvez GM. Pharmacological activities of mango (Mangifera Indica): A review. J Pharmacognosy Phytochemistry 2016; 5(3): 1-7
22. Gondi M, Basha SA, Bhaskar JJ, Salimath PV, Rao UJ. Anti-diabetic effect of dietary mango (Mangifera indica L.) peel in streptozotocin-induced diabetic rats. J Sci Food Agric 2015; 95(5): 991-9.
23. Mohanvelu R, Madhuri AS, Ramabhimaiah S. Evaluation of analgesic activity of aqueous extract of Mangifera indica leaves in albino rats. Int J Basic Clin Pharmacol 2015; 4(1): 107-10.
24. Rivera DG, Balmaseda IH, León AA, Hernández BC, Montiel LM, et al. Anti-allergic properties of Mangifera indica L. extract (Vimang) and contribution of its glucosylxanthone mangiferin. J Pharm Pharmacol 2006; 58(3): 385-92.
25. Prabhu K, Rajan S. Assessment of Antiulcer Activity of Ethanolic Extract of Mangifera indica Seed Kernel Using Acid Ethanol Induced Ulcer Model. Int J Current Microbiol App Sci 2015; 4(4): 854-60.
26. Yakubu MT, Salimon SS. Antidiarrhoeal activity of aqueous extract of Mangifera indica L. leaves in female albino rats. J Ethnopharmacol 2015; 163: 135-41.
27. Pallab K. An Overview on Mangifera Indica Importance and Its Various Pharmacological Action. PharmaTutor 2014; 2(12): 72-6.
28. Beltrán AE, Alvarez Y, Xavier FE, Hernanz R, Rodriguez J, et al. Vascular effects of the Mangifera indica L. extract (Vimang). Eur J Pharmacol 2004; 499(3): 297-305.
29. Ahmed A, Eltyeb I, Mohamed A. Pharmacological activities of Mangifera indica Fruit Seeds Methanolic Extract. Omdurman J Pharmaceutical Sci 2006; 1(2): 216-31.
30. Alkizim FO, Matheka D, Abdulrahman FK, Muriithi A. Inhibitory effect of Mangifera indica on gastrointestinal motility. J Med Chem Drug Disco 2012; 2(1): 9-16.
31. Agbonon A1, Eklu-Gadegbeku K, Aklikokou K, Essien K, Akpagana K, Gbeassor M. The effect of Mangifera indica stem bark and Pluchea ovalis roots on tracheal smooth muscle in vitro. Fitoterapia 2002; 73(7-8): 619-22.
32. Dehghan Shiebani M, Noureddini M. Effect of Aqueous Extract of Mangifera Indica Seed on the Basic Contractility, Frequency and Strength of Isolated Virgin Rat Uterine Smooth Muscle. CHJ. 2014; 8(2): 66-73.]
33. Sairam K, Hemalatha S, Kumar A, Srinivasan T, Ganesh J, Shankar M, et al. Evaluation of anti-diarrhoeal activity in seed extracts of Mangifera indica. J Ethnopharmacol 2003; 84(1): 11-5.
34. Maggio CD, Jennings SR, Robichaux JL, Stapor PC, Hyman JM. A modified Hai-Murphy model of uterine smooth muscle contraction. Bull Math Biol 2012; 74(1): 143-58.
35. Keskil Z, Bayram M, Ercan ZS, Türker RK. The contribution of nitric oxide and endothelins to angiotensin II. Evoked responses in the rat isolated uterus smooth muscle. Gen Pharmacol 1999; 33(4): 307-12.
36. Bausher LP, Gregory DS, Sears ML. Interaction between alpha 2- and beta 2-adrenergic receptors in rabbit ciliary processes. Curr Eye Res 1987; 6(3): 497-505.
37. after cervical ganglionectomy. Curr Eye Res 1991; 10(5): 399-407.
38. Ahangarpour A, Amirzargar A, Oroojan AA, Bakhtiyarizadeh H, Nikraftar O. The effect of Daucus carota aqueous extract on uterine contractions of non-pregnant rats. Daneshvar Med 2012; 19(3): 67-74. [in Persian]
39. Yektaparast-Movafegh K, Panahi N, Sadeghi Hashjin G. Antispasmodic effect of Ephedra major extract on adrenergic and calcium channels receptors in the rat uterus. J Comparative Pathol 2015; 12(1): 1569-75. [in Persian]
40. Cole NM, Carvalho JC, Erik-Soussi M, Ramachandran N, Balki M. In Vitro Comparative Effect of Carbetocin and Oxytocin in Pregnant Human Myometrium with and without Oxytocin Pretreatment. Anesthesiology 2016; 124(2): 378-86.
41. Gruber CW, O'Brien M. Uterotonic plants and their bioactive constituents. Planta Med 2011; 77(3): 207-20.
42. Kitazawa T, Hirama R, Masunaga K, Nakamura T, Asakawa K, Cao J, et al. Muscarinic receptor subtypes involved in carbachol-induced contraction of mouse uterine smooth muscle. Naunyn Schmiedebergs Arch Pharmacol 2008; 377(4-6): 503-13.
43. Abdalla FM, Maróstica E, Picarelli ZP, Abreu LC, Avellar MC, Porto CS. Effect of estrogen on muscarinic acetylcholine receptor expression in rat myometrium. Mol Cell Endocrinol 2004; 213(2): 139-48.
44. Kitazawa T, Hashiba K, Cao J, Unno T, Komori S, Yamada M, et al. Functional roles of muscarinic M2 and M3 receptors in mouse stomach motility:studies with muscarinic receptor knockout mice. Eur J Pharmacol 2007; 554(2-3): 212-22.
45. Yasuda K, Sumi G, Kanamori C, Nakajima T, Tsuzuki T, Cho H, et al. Effects of ovarian hormone treatment on the gene expression of muscarinic acetylcholine receptors in the ovariectomized rat myometrium. J Steroid Biochem Mol Biol 2014; 143: 81-9.
46. Agbonon A, Aklikokou K, Gbeassor M. Mangifera indica stem bark effect on the rat trachea contracted by acetylcholine and histamine. Pharm Biol 2005; 43(5): 475–9.
47. Alani B, Zare M, Noureddini M. Bronchodilatory and B-adrenergic effects of methanolic and aqueous extracts of Althaea root on isolated tracheobronchial smooth rat muscle. Adv Biomed Res 2015; 4: 78.
48. Al-Otaibi MA. The physiological mechanism of uterine contraction with emphasis on calcium ion. Calcium Signaling 2014; 1(2): 70-5.
49. Brainard AM, Korovkina VP, England SK. Potassium channels and uterine function. Semin Cell Dev Biol 2007; 18(3): 332-9.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Noureddini M, Dehghan-Sheibani M, Alani B. Effect of aqueous extract of mango kernel (Mangifera Indica) on basal activity of virgin rat uterine smooth muscle: role of muscarinic receptors. Feyz. 2017; 21 (1) :19-27
URL: http://feyz.kaums.ac.ir/article-1-3276-en.html


Volume 21, Issue 1 (Bimonthly 2017) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3921