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:: Volume 21, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 21(6): 584-590 Back to browse issues page
Investigation of c-Myc gene amplification in breast cancer patients and its correlation with other prognostic factors
Zohreh Zahraei , Atefeh Sarlak , Mohammad Esmaeel Akbari
Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, I. R. Iran. , zahraie@kashanu.ac.ir
Abstract:   (5135 Views)
Background: Breast cancer is the most common cancer in woman. The frequency of amplification of some proto-oncogenes like c-Myc gene may be various in different populations.  In this study, amplification of c-Myc oncogene was determined in breast cancer patients and its correlation with prognostic factors such as age, tumor size, tumor stage, grade of the disease, lymph node involvement, HER/2 protein expression, estrogen and progesterone receptors and recurrence was also investigated.
Materials and Methods: In this study, to determine the amplification of c-Myc oncogenes in breast cancer patients, the multiplex PCR technique was used. After extracting DNA from 100 tumor tissue and 8 normal breast tissue samples, amplification of c-Myc gene was determined by coamplification of a single-copy reference gene, g-IFN, and the target gene c-myc in PCR reaction and using the Gel analyzer software. In the next step, the correlation of the amplification of this gene with other prognostic factors was investigated.
Results: In this study, amplification of the c-Myc gene was observed in 27% of the tumor samples. The statistic analysis showed that amplification of the c-Myc oncogene was significantly associated with the recurrence of breast cancer. There was no significant correlation between amplification of the c-Myc oncogene and other prognostic factors including age, estrogen and progesterone receptors, lymph node involvement, HER/2 expression, tumor size, stage and grade of the disease.
Conclusion: Amplification of the c-Myc gene can be used as an independent prognostic factor in predicting the recurrence in breast cancer patients.
Keywords: Breast cancer, Gene amplification, c-Myc oncogene, Prognostic factors
Full-Text [PDF 351 kb]   (1955 Downloads)    
Type of Study: Applicable | Subject: medicine, paraclinic
Received: 2017/02/17 | Revised: 2018/01/10 | Accepted: 2017/11/18 | Published: 2017/12/12
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Zahraei Z, Sarlak A, Akbari M E. Investigation of c-Myc gene amplification in breast cancer patients and its correlation with other prognostic factors. Feyz 2017; 21 (6) :584-590
URL: http://feyz.kaums.ac.ir/article-1-3267-en.html


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Volume 21, Issue 6 (Bimonthly 2017) Back to browse issues page
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