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:: Volume 11, Issue 2 (Quarterly 2007) ::
Feyz 2007, 11(2): 6-11 Back to browse issues page
Effect of vitrification with single agent in stepwise manner using different concentrations of ethylene gycol on the morphology of mouse ovarian tissue
Elaheh Mirrasekhian, Mogdeh Salehnia *
Tarbiat Modarres University of Medical Sciences , mogdeh@dr.com
Abstract:   (16168 Views)

Background: Considering the importance of cryoprotection and dehydration manner in the vitrification procedure, the aim of this study was to evaluate the effect of this technique using different concentrations of ethylene glycol (EG) as a single agent in stepwise manner and different dehydration time on the mouse ovarian tissue.

Materials and Methods: Thirty five NMRI adult mice (6-10 weeks) were sacrificed and their ovarian tissues were divided into control, toxicity test and vitrified groups. After dehydration of samples in increasing concentration of EG (2 and 4 M) for 1 min in every group the vitrification procedure was done using final concentration of 6 or 8 M of EG in different times (4,6, 8 min). The ovarian tissue in each vitrified group was stored in liquid nitrogen for one week. After warming, the samples were washed in 1 M sucrose solution. The samples of fresh, vitrified and toxicity test groups were fixed in 10% formaldehyde solution and embedded in paraffin. Then serial sections were prepared, stained with hematoxylen and eosin and studied morphologically.

Results: The morphology of ovarian tissue in all groups with different concentrations of EG and different dehydration times was almost the same. The preantral and antral follicles were well preserved especially on the cortical part of ovary they had a normal morphology.

Conclusion: The results showed that high concentration of EG (6 or 8 M) was not toxic and dehydration time from 4 to 8 minutes had no harmful effect on the structure of ovarian tissue during vitrification and warming. However, more additional investigations are recommended.

Keywords: Ethylene glycol, Ovarian tissue, Stepwise manner vitrification
Full-Text [PDF 210 kb]   (1374 Downloads)    
Type of Study: Research | Subject: General
Received: 2008/10/26 | Published: 2007/07/15
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Mirrasekhian E, Salehnia M. Effect of vitrification with single agent in stepwise manner using different concentrations of ethylene gycol on the morphology of mouse ovarian tissue. Feyz. 2007; 11 (2) :6-11
URL: http://feyz.kaums.ac.ir/article-1-32-en.html


Volume 11, Issue 2 (Quarterly 2007) Back to browse issues page
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