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Cytotoxicity effects of green synthesized silver nanoparticles on human colon cancer (HT29) cells
Rabee Movagharnia , Fahimeh Baghbani-Arani *, Seyed Ataollah Sadat Shandiz
, baghbani.f@gmail.com
Abstract:   (450 Views)

Background: Nowadays, investigations on the role of nanoparticles for diagnosis, novel drug delivery system design and treatment of cancers are being increased. The aim of this study was to evaluate the cytotoxicity effects of green synthesized AgNPs by using Artemisia on human colon cancer (HT29) cells.

Materials and Methods: In the current study, the eco-friendly green synthesis of AgNPs by using Artemisa tournefortiana was evaluated. The characterization of fabricated AgNPs was performed by Ultraviolet-visible (UV-vis) spectroscopy, X-Ray Diffraction (XRD), and Transmission Electron Microscopy (TEM). The HT29 and HEK293 cell lines were treated with various concentrations of synthesized AgNPs for 24 hours. The viability effect of cells and Half maximal inhibitory concentration) IC50(were calculated by MTT assay.

Results: The fabricated AgNPs were monitored characteristic surface plasmon resonance peak at around 425nm. The crystallographic shapes of AgNPs were evaluated by XRD results. The TEM result for shape and size of AgNPs was show that the nanoparticles are ranging from 10 to 30 nm. The MTT results demonstrated that AgNPs significantly decreased the viability of cells in dose-and time-dependent manner. The IC50 value of nanoparticles for HT29 and HEK293 cell lines were calculated 40.71 and 61.38 mg/mL during the 24 hours respectively.

Conclusion: According to the current study, the green fabricated AgNPs can more cytotoxic effect against colon cancer cells compared to the normal cells. Thus, they can be considered as a promising strategy for the treatment of colon cancer.

Keywords: Green synthesis, silver nanoparticles, MTT, colon cancer
     
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/12/8 | Accepted: 2017/10/30 | Published: 2017/12/12
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Movagharnia R, Baghbani-Arani F, Sadat Shandiz S A. Cytotoxicity effects of green synthesized silver nanoparticles on human colon cancer (HT29) cells. Feyz. 2018; 22 (1)
URL: http://feyz.kaums.ac.ir/article-1-3196-en.html


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