[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 2 (Bimonthly 2016) ::
Feyz 2016, 20(2): 125-132 Back to browse issues page
Effect of hydroalcoholic extract of Ferula szowitsiana DC. on paw edema in rat
Seyyed Javad Saghravanian, Masoud Fereidoni *, Ali Asadollahi
Rayan Center for Neuroscience and Behavior, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, I. R. Iran. , fereidoni@um.ac.ir
Abstract:   (3165 Views)

Background: Due to the side effects of chemical medicines, nowadays the use of drugs of natural origin is concerned. In traditional medicine plants of Ferula family generally regarded as the inhibitor of pain and inflammation. In this study using the sub-plantar formalin injection the anti-inflammatory effect of the hydroalcoholic extract of Ferula szowitsiana DC., as one of the species of this family, is investigated on rat paw edema.

Materials and Methods: The hydroalcoholic extract of Ferula szowitsiana DC. was prepared and solved in Saline, Ethanol and Tween 80 (in a ratio of 8:1:1, respectively). Male Wistar rats (200-250 g) were assigned to: Control, Solvent (i.p), Solvent (i.t), Extract (50, 100, 200, 400 mg/kg, i.p), Naloxone (2 mg/kg, i.p), Naloxone-Extract (2 mg/kg, i.p and 400 mg/kg, i.p, respectively) and Extract (8 μg/10 μl, i.t) groups. For inducing inflammation Formalin 2.5% (0.05 ml) and for assessing the volume of edema plethysmometeric methods were used.

Results: The extract of Ferula szowitsiana DC. reduced inflammation in a dose dependent manner (P<0.001). The effects of central (i.t.) administration of the equivalent concentration of the effective dose of extract were similar to their systemic administration. Naloxone partially reduced the anti-inflammatory effect of extract (P<0.05).

Conclusion: The anti-inflammatory property of Ferula szowitsiana DC. extract is probably induced because of its effect on central nervous system (CNS) and part of this effect is mediated by opioid system.

Keywords: Ferula szowitsiana DC., Inflammation, Plethysmometer, Rat
Full-Text [PDF 297 kb]   (967 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/06/16 | Accepted: 2016/06/16 | Published: 2016/06/16
References
1. Richardson JD, Vasko MR. Cellular mechanisms of neurogenic inflammation. J Pharmacol Exp Ther 2002; 302(3): 839-45.
2. Kandel ER, Schwartz JH, Jessell TM, Siejelbaum SA, Hudspeth AJ. Principles of neural science. 5th ed. New York: McGraw-Hill Companies; 2013. p. 530-55.
3. Sharififar F, Koohpayeh A, Motaghi MM, Amirkhosravi A, Puormohseni Nasab E, Khodashenas M. Study the ethnobotany of medicinal plants in Sirjan, Kerman province, Iran. J Herbal Drugs 2010; 1(3): 19-28.
4. Dehghan G, Solaimanian R, Shahverdi AR, Amin G, Abdollahi M, Shafiee A. Chemical composition and antimicrobial activity of essential oil of Ferula szovitsiana D.C. Flavour Frag J 2007; 22: 224-7.
5. Habibi Z, Aghaie HR, Ghahremanzadeh R, Masoudi S, Rustaiyan A. Composition of the essential oils of Ferula szowitsiana DC., Artedia squamata L. and Rhabdosciadium petiolare Boiss. & Hausskn.ex Boiss. three umbelliferae herbs growing wild in Iran. J Essent Oil Res 2006; 18: 503-5.
6. Kim DS, Lee HJ, Jeon YD, Han YH, Kee JY, Kim HJ, et al. Alpha-Pinene Exhibits Anti-Inflammatory Activity Through the Suppression of MAPKs and the NF-kappaB Pathway in Mouse Peritoneal Macrophages. Am J Chin Med 2015; 43(4): 731-42.
7. Liapi C, Anifandis G, Chinou I, Kourounakis AP, Theodosopoulos S, Galanopoulou P. Antinociceptive properties of 1,8-Cineole and beta-pinene, from the essential oil of Eucalyptus camaldulensis leaves, in rodents. Planta Med 2007; 73(12): 1247-54.
8. Karlsten R, Gordh T, Post C. Local antinociceptive and hyperalgesic effects in the formalin test after peripheral administration of adenosine analogues in mice. Pharmacol Toxicol 1992; 70(6 Pt 1): 434-8.
9. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983; 16(2): 109-10.
10. Fereidoni M, Ahmadiani A, Semnanian S, Javan M. An accurate and simple method for measurement of paw edema. J Pharmacol Toxicol Methods 2000; 43(1): 11-4.
11. Ahmadiani A, Fereidoni M, Semnanian S, Kamalinejad M, Saremi S. Antinociceptive and anti-inflammatory effects of Sambucus ebulus rhizome extract in rats. J Ethnopharmacol 1998; 61(3): 229-35.
12. Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Dehpour AA. Antioxidant activity of hydroalcholic extract of Ferula gummosa Boiss roots. Eur Rev Med Pharmacol Sci 2011; 15(6): 658-64.
13. Urushidani T, Forte JG, Sachs G. Mechanisms and consequences of proton transport. Boston, Mass.; London: Kluwer Academic; 2002.
14. Yaksh TL, Rudy TA. Chronic catheterization of the spinal subarachnoid space. Physiol Behav 1976; 17(6): 1031-6.
15. Alizadeh Z, Fereidoni M, Behnam-Rassouli M, Hosseini S. Role of C-fibers in pain and morphine induced analgesia/hyperalgesia in rats. Iran J Neurol 2014; 13(1): 19-27.
16. de Cassia da Silveira e Sa R, Andrade LN, de Sousa DP. A review on anti-inflammatory activity of monoterpenes. Molecules 2013; 18(1): 1227-54.
17. Merighi S, Gessi S, Varani K, Fazzi D, Mirandola P, Borea PA. Cannabinoid CB(2) receptor attenuates morphine-induced inflammatory responses in activated microglial cells. Br J Pharmacol 2012; 166(8): 2371-85.
18. Abo-Salem OM, Hayallah AM, Bilkei-Gorzo A, Filipek B, Zimmer A, Muller CE. Antinociceptive effects of novel A2B adenosine receptor antagonists. J Pharmacol Exp Ther 2004; 308(1): 358-66.
19. Gutierrez-Venegas G, Arreguin-Cano JA, Hernandez-Bermudez C. Bradykinin promotes Toll like receptor-4 expression in human gingival fibroblasts. Int Immunopharmacol 2012; 14(4): 538-45.
20. Hosseinzadeh H, Behravan E, Soleimani MM. Antinociceptive and anti-inflammatory effects of Pistacia vera leaf extract in mice. Iran J Pharm Res 2011; 10(4): 821-8.
21. Kiasalari Z, Khalili M, Roghani M, Heidari H, Azizi Y. Antiepileptic and Antioxidant Effect of Hydroalcoholic Extract of Ferula Assa Foetida Gum on Pentylentetrazole- induced Kindling in Male Mice. Basic Clin Neurosci 2013; 4(4): 299-306.
22. Damas J, Liegeois JF. The inflammatory reaction induced by formalin in the rat paw. Naunyn Schmiedebergs Arch Pharmacol 1999; 359(3): 220-7.
23. Borner C, Hollt V, Kraus J. Mechanisms of the inhibition of nuclear factor-kB by morphine in neuronal cells. Mol Pharmacol 2012; 81(4): 587-97.
24. Franchi S, Moretti S, Castelli M, Lattuada D, Scavullo C, Panerai AE, et al. Mu opioid receptor activation modulates Toll like receptor 4 in murine macrophages. Brain Behav Immun 2012; 26(3): 480-8.
25. Morello S, Sorrentino R, Pinto A. Adenosine A2a receptor agonists as regulators of inflammation: pharmacology and therapeutic opportunities. J Receptor Ligand Channel Res 2009; 2: 11-7.
26. Kidd BL, Urban LA. Mechanisms of inflammatory pain. Br J Anaesth 2001; 87(1): 3-11.
27. Fredholm BB, Cunha RA, Svenningsson P. Pharmacology of adenosine A2A receptors and therapeutic applications. Curr Top Med Chem2003; 3(4): 413-26.
28. Freidin M, Kessler JA. Cytokine regulation of substance P expression in sympathetic neurons. Proc Natl Acad Sci U S A 1991; 88(8): 3200-3.
29. Hua XY, Chen P, Fox A, Myers RR. Involvement of cytokines in lipopolysaccharide-induced facilitation of CGRP release from capsaicin-sensitive nerves in the trachea: studies with interleukin-1beta and tumor necrosis factor-alpha. J Neurosci 1996; 16(15): 4742-8.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Saghravanian S J, Fereidoni M, Asadollahi A. Effect of hydroalcoholic extract of Ferula szowitsiana DC. on paw edema in rat. Feyz. 2016; 20 (2) :125-132
URL: http://feyz.kaums.ac.ir/article-1-3028-en.html


Volume 20, Issue 2 (Bimonthly 2016) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3937