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:: Volume 21, Issue 4 (Bimonthly 2017) ::
Feyz 2017, 21(4): 390-397 Back to browse issues page
Prevalence of metallo-beta-lactamase enzyme and patern of antibiotic resistance in Klebsiella pnomoniae isolated from patients with urinary tract infection in Qom city during 2013-2014
Rohollah Khodadadian, Ahmad Khorshidi *, Mahmoud Safari, Hamid Reza Gilasi
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R. Iran. , khorshidimalahmadi@gmail.com
Abstract:   (1887 Views)
Bachground: Klebsiella pnomoniae is one of the most important etiologic agents of urinary tract infection (UTI). An increasing occurrence of antimicrobial resistance among uropathogenic bacterial isolates has complicated the treatment process. The aim of this study was to determine antibiotic susceptibility patterns and prevalence of the metallo-beta-lactamase enzyme of K. pneumoniae isolates collected from UTI.
Materials and Methods: This cross-sectional study was conducted on patients with complicated UTI reffered to hospitals in Qom city, Iran. A total of 1807 culture positive samples of pathogens were collected from the patients, among which 457 isolates were K. pneumoniae. The isolates were tested for antimicrobial susceptibility by the disc-diffusion method recommended by the guidelines of Clinical and Laboratory Standards Institute (CLSI 2013). In addition,  the dubble disk synergy test was used to detect the K. pneumoniae isolates of metallo-beta-lactamase enzyme.
Results: The prevalence of UTI infection due to K. pneumoniae was 25.3%. Among 1807 positive urine cultures, 62.4% were from females and 37.6% from males. Results of antimicrobial susceptibility showed that the highest antibiotic resistance was seen for trimetoprium-sulfametoxazole (98.5%) and the lowest resistance levels were seen for amikacin (9.4%), meropenem (22.8%) and  imipenem (25.6%). The results of the imipenem-EDTA combined disk showed that 93.2% imipenem resistance isolates were positive for the metallo-beta- lactamase enzyme.
Conclusion: Carbapenem resistance and production of the metallo-beta-lactamase enzyme in K. pneumoniae uropathogenic are increasing. However, amikacin is still effective against these bacterial infections and its effectiveness should be maintained.
Keywords: Klebsiella pnomoniae, UTI, Antibiotic resistance, Metallo-Beta-Lactamases
Full-Text [PDF 269 kb]   (603 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/04/8 | Accepted: 2017/07/18 | Published: 2017/10/14
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Khodadadian R, Khorshidi A, Safari M, Gilasi H R. Prevalence of metallo-beta-lactamase enzyme and patern of antibiotic resistance in Klebsiella pnomoniae isolated from patients with urinary tract infection in Qom city during 2013-2014. Feyz. 2017; 21 (4) :390-397
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Volume 21, Issue 4 (Bimonthly 2017) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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