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:: Volume 19, Issue 1 (Bimonthly 2015) ::
Feyz 2015, 19(1): 67-75 Back to browse issues page
Relationship between the IL28B gene-related single-nucleotide polymorphism of rs8099917 and susceptibility to hepatitis C infection in Iranian population
Mona Sadat-Larijani, Mehri Nikbin, Gholam Reza Bakhshi-Khaniki, Solmaz Talebi, Mohammad Reza Aghasadeghi, Sayyed Mehdi Sadat *
Department of Hepatitis and AIDS, Pasteur Institute of Iran , mehdi_sadat@pasteur.ac.ir
Abstract:   (5418 Views)
Background: The current medical treatment for hepatitis C is a combination of antiviral therapy along with pegylated interferon alpha and ribavirin. Recent studies have demonstrated that single nucleotide polymorphisms near the interleukin 28B gene coding for IFN-λ3 were associated with the antiviral response. Therefore, this study aimed to determine the frequency of G/T polymorphism of rs8099917 among the Iranian population.
Materials and Methods: This cross-sectional study was performed on 93 blood samples (71 sensitive and 22 resistant to treatment) collected from individuals suffering from chronic HCV, and 57 healthy controls. DNA was extracted from the samples and the frequency of the polymorphism was analyzed using the PCR-RFLP method. Finally, the products were detected on 3.5% agarose gel electrophoresis.
Results: The frequency of the G/T polymorphism between the healthy individuals and patients were TT: 75%, TG: 23%, GG: 2%, and TT: 57%, TG: 35%, GG: 8%, respectively. Moreover, the TT genotype was identified in 46 patients of whom 71 achieved SVR, while the GT heterozygous was found in 33 patients and SVR was achieved in 19. Finally, the GG was detected in 7 patients and only one patient was resistant to treatment.
Conclusion: Results show a significant effect of G allele on susceptibility to HCV compared to the other allele T (P=0.013). Although no correlation was seen between the polymorphism and SVR among the patients, further studies with more samples are necessary.
Keywords: Hepatitis C, IL-28B gene, Polymorphism, rs8099917
Full-Text [PDF 327 kb]   (1476 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2015/03/4 | Accepted: 2015/03/4 | Published: 2015/03/4
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Sadat-Larijani M, Nikbin M, Bakhshi-Khaniki G R, Talebi S, Aghasadeghi M R, Sadat S M. Relationship between the IL28B gene-related single-nucleotide polymorphism of rs8099917 and susceptibility to hepatitis C infection in Iranian population. Feyz. 2015; 19 (1) :67-75
URL: http://feyz.kaums.ac.ir/article-1-2536-en.html


Volume 19, Issue 1 (Bimonthly 2015) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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