[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Issue 5 (Quarterly 2013) ::
Feyz 2013, 17(5): 413-421 Back to browse issues page
Investigating the effects of low-frequency electromagnetic field on HT-29 cancer cell line
Sayyed Damoon Sadooghi *
Islamic Azad University , damoon.sadughi@gmail.com
Abstract:   (4782 Views)

Background: Colon cancer is the most common digestive cancer and also the fourth cause of cancer death around the world. On the other hand, many processes can be affected by electromagnetic fields and such fields may have different effects on cells.The aim of this study was to investigate the effect of low-frequency electromagnetic field on HT-29 cancer cell line.

Materials and Methods: HT-29 and L929 cell lines were cultured in RPMI medium containing fetal bovine serum and antibiotics. Cells were exposed to 50 Hz electromagnetic field with intensities of 50, 200 and 400 gauss for 3 hours. Growth, proliferation and morphological changes were photographed using an inverted microscope. MTT assay was used to quantify cell viability.

Results: Viability percent of HT-29 (81.097±2.703) and L929 (89.375±3.672) cell lines in the vicinity of electromagnetic field with intensity of 50 gauss was not significantly decreased compared to the control group. Viability percent of HT-29 (30.807±4.479) and L929 (35.179±4.137) cell lines in the vicinity of electromagnetic field with intensity of 200 gauss was significantly decreased compared to the control group. Moreover, viability percent of HT-29 (18.391±3.091) and L929 (23.046±3.513) cell lines in the vicinity of electromagnetic field with intensity of 400 gauss was significantly decreased compared to the control group.

Conclusion: Electromagnetic field with frequency of 50 Hz and intensities of 200 and 400 gauss has an inhibitory effect on the growth and proliferation as well as a cytotoxic effect on HT-29 and L929 cell lines.

Keywords: HT-29, Cancer, Electromagnetic field, MTT assay
Full-Text [PDF 320 kb]   (2115 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/09/30 | Accepted: 2013/10/19 | Published: 2013/10/19
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadooghi S D. Investigating the effects of low-frequency electromagnetic field on HT-29 cancer cell line. Feyz. 2013; 17 (5) :413-421
URL: http://feyz.kaums.ac.ir/article-1-2045-en.html


Volume 17, Issue 5 (Quarterly 2013) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3937