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:: Volume 17, Issue 3 (Bimonthly 2013) ::
Feyz 2013, 17(3): 294-299 Back to browse issues page
A study on the prevalence of rotavirus infection among the HIV-positive patients with gastroenteritis
Mohammad Hasan Pouriayevali , Mehdi Zavvar , Mohammad Reza Aghasadeghi , Sayed Mehdi Sadat , Alireza Azizi-Saraji , Fozieh Javadi , Javid Sadraraei , Ehsan Mostafavi , Fatemeh Motevalli , Pooneh Rahimi
Pasteur Institute of Iran , mehdi_sadat@pasteur.ac.ir
Abstract:   (5917 Views)

Background: Gastroenteritis is one of the most common symptoms in AIDS patients. Although gastroenteritis in these patients is caused by several factors, the role of viral agents, especially rotavirus, is still unknown. The aim of this study was to evaluate the prevalence of rotavirus infection among the HIV-positive cases.

Materials and Methods: In this cross-sectional study, 75 fecal samples were collected from HIV-positive patients with gastroenteritis referred to Imam Khomeini hospital. After viral RNA extraction, the sixth conserved segment of the virus genome (VP6) was amplified using RT-PCR method. Finally, the products were detected on 1.5% agarose gel electrophoresis and confirmed by sequencing.

Results: RT-PCR products with the expected size (433bp) were obtained for all rotavirus- positive as well as the wild-type standard viral isolates. Among the samples taken from 75 HIV-positive cases, 19 (25.3%) were rotavirus-positive and confirmed using direct sequencing.

Conclusion: Although in this study the anticipated prevalence of rotavirus among the HIV-positive cases is about 25%, further studies are required to characterize the genotype of rotavirus in HIV- positive cases with gastroenteritis.

Keywords: Rotavirus, HIV, Gastroenteritis
Full-Text [PDF 275 kb]   (2135 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/06/27 | Revised: 2013/07/7 | Accepted: 2013/07/7 | Published: 2013/07/7
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Pouriayevali M H, Zavvar M, Aghasadeghi M R, Sadat S M, Azizi-Saraji A, Javadi F, et al . A study on the prevalence of rotavirus infection among the HIV-positive patients with gastroenteritis. Feyz 2013; 17 (3) :294-299
URL: http://feyz.kaums.ac.ir/article-1-1960-en.html


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This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
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Volume 17, Issue 3 (Bimonthly 2013) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
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