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:: Volume 17, Issue 2 (Bimonthly 2013) ::
Feyz 2013, 17(2): 188-194 Back to browse issues page
Prevalence of Shiga toxin and Intimine genes in Shiga toxin-producing Escherichia coli isolated from urine samples in Lorestan, Iran
Zahra Adeli, Farzaneh Firoozeh *, Mohammad Zibaei, Peghah Shakib
Kashan University of Medical Sciences , ffiroozeh@ut.ac.ir
Abstract:   (4772 Views)

Background: Shiga toxin-producing Escherichia coli (STEC), as one of the most important food-borne pathogen, in human may lead to deadly syndromes, like hemolytic–uremic syndrome (HUS). Occurrence of HUS following urinary tract infection (UTI) has been previously reported. The aim of this study was to identify stx1, stx2 and eaeA genes in E. coli strains isolated from urine samples in Alashtar (Lorestan, Iran)

Materials and Methods: A total number of 144 bacterial isolates were collected from three hospitals in Alashtar during a six-month period. One-hundred E. coli isolates were identified using the standard biochemical tests as well as the selective and differential media. The multiplex PCR method was used to evaluate the presence of stx1, stx2 and eaeA genes.

Results: Two out of 100 E. coli isolates carried both stx2 and eaeA genes and one isolate (1%) only sxt1gene. Moreover, the three genes were not found in any of the isolates tested.

Conclusion: Detection of Shiga toxin-producing E. coli (e.g. O157:H7 and non-O157:H7 serotypes), which may lead to life-threatening syndromes such as HUS, from urine samples is of great importance . Further research in this field using the fast and precise molecular methods is recommended.

Keywords: E. coli, Shiga toxin, Intimine, Urinary tract infection
Full-Text [PDF 219 kb]   (2409 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/05/5 | Accepted: 2013/05/15 | Published: 2013/05/15
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Adeli Z, Firoozeh F, Zibaei M, Shakib P. Prevalence of Shiga toxin and Intimine genes in Shiga toxin-producing Escherichia coli isolated from urine samples in Lorestan, Iran. Feyz. 2013; 17 (2) :188-194
URL: http://feyz.kaums.ac.ir/article-1-1906-en.html


Volume 17, Issue 2 (Bimonthly 2013) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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