Background: Prior studies have proposed that neonates do feel pain. Venepuncture is one the most painful procedures for neonates. There are several ways to eliminate this pain. The present study was conducted to compare the effects of glucose 33%, breast milk and lidocaine cream on acute pain of arteriopuncture in term neonates hospitalized in Shabih Khani and Shaheed Beheshti hospitals in Kashan.

Materials and methods: A clinical trial was performed on 120 term neonates. They were assigned in four groups (each 30). First group underwent blood sampling without any particular treatment. Blood sampling was performed after 2cc of breast milk in the second, 2cc of glucose 33% solution in the third and application of a thin layer of 2% lidocaine cream in the fourth group, respectively. During the blood sampling process DAN score and crying period were determined.

Results: The mean crying period were 65.33±45.97, 48.83±35.78, 18.60±16.65, and 24.67±23.19 in the control, breast milk, Lidocaine cream and glucose groups , respectively (p<0.0001). DAN score showed a significant difference between groups according to the Kruskal-Wallis analysis (p<0.0001).

Conclusion : Both glucose solution and lidocaine cream decreased pain severity and crying period, however, due to safer state of glucose solution, it is more strongly recommended.