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:: Volume 16, Issue 7 (Supplement 2013) ::
Feyz 2013, 16(7): 737-738 Back to browse issues page
Determining the effect of trace elements (Lead and Zinc) on amylin Amyloidogenesis in vitro
Sayyed Mahdi Mirhashemi , Fereshteh Bahmani , Fatemeh Nejati , Fahimeh Talebi , Farzaneh Khalaji , Mohsen Mahdizade
Kashan University of Medical Sciences , mirhashemi@kaums.ac.ir
Abstract:   (6274 Views)

Background: Type 2 diabetes mellitus can be defined as a conformational disease because in which a constituent beta cell protein, islet amyloid polypeptide (amylin), undergoes a change in tertiary structure followed by a self-association. In this research, Lead (Pb) and Zinc effects in the aggregation of the amylin was examined experimentally.

Materials and Methods: Human amylin peptide and other chemicals were purchased from Sigma-Aldrich Company. Aggregation was induced by adding stock solution to modified Krebs-Hensleit buffer (pH: 7.4) to a final concentration of 0.4 μM and incubated at 37oC for 144h. Zinc (50 μM) and Pb (10 μM) was prepared in the peptide containing solution, separately. Also a mixture of trace elements and amylin with the same concentration was prepared. The zinc and Pb free solution was selected as control. To monitor the peptide precipitation Thioflavin T fluorescence assay was performed.

Results: The results showed that zinc increased amylin aggregation by 12.31 % (P<0.05) and Pb enhanced aggregation potential by 23.17 % (P<0.05). A combination of Zn and Pb increased amylin aggregation by 0.24% compared to Pb group.

Conclusion: Under the experimental conditions, Pb and Zinc separately have permissive effects on formation of beta-amyloid sheet. However, pb along with Zinc has no synergistic effect on aggregation of amylin.

Keywords: Diabetes Mellitus, Amylin, Lead, Zinc
Full-Text [PDF 138 kb]   (1152 Downloads)    
Type of Study: Research | Subject: General
Received: 2013/03/4 | Revised: 2013/03/4 | Published: 2013/03/15
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Mirhashemi S M, Bahmani F, Nejati F, Talebi F, Khalaji F, Mahdizade M. Determining the effect of trace elements (Lead and Zinc) on amylin Amyloidogenesis in vitro. Feyz 2013; 16 (7) :737-738
URL: http://feyz.kaums.ac.ir/article-1-1835-en.html

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Volume 16, Issue 7 (Supplement 2013) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
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