Background: Lead pollution is produced by industrial processes and smoke emitted by vehicles. It negatively affects the physiological processes such as hematopoietic system, immune system, kidney and the nervous system. However, its mechanism on blood parameters is not yet known. This study aimed to analyze the effect of Lead on coagulation and fibrinolysis in healthy subjects in vitro.
Materials and Methods: In this study, 0.5, 1, 4, and 5 µ M of Lead acetate were added to plasma citrate . It was incubated at 37 ° C for 24 hours. Then, coagulation and fibrinolysis parameters were analyzed by adding Phosphate buffer, Calcium chloride, and Streptokinase enzyme applying turbidimetric procedure at 405nm.
Results: The following results were achieved by comparing the control group with 0.5, 1, 4, and 5 µ M of Lead acetate, respectively:
The total coagulation time is (823±42.40), (753±38.30), (742±46.42), (731±54.58), (716 ±59.83), lysis maximum velocity (0.144±0.011), (0.128±0.004), (0.111±0.004), (0.105±0.003), (0.097±0.017), and the total lysis time except 0.5 µ M (342±26.37), (424±18.40), (460±19.20), (476±23.12) which changed significantly (P<0.05), and lag time of fibrinolysis, halftime lysis, coagulation maximum velocity increased and delay coagulation time decreased, but were not significant (P >0.05).
Conclusion: The shortening of coagulation duration and fibrinolysis extension in the presence of high concentrations of Lead can be a reason for cardiovascular diseases in people exposed to Lead.