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:: Volume 16, Issue 5 (Bimonthly 2012) ::
Feyz 2012, 16(5): 454-460 Back to browse issues page
Expression of the apoptotic genes Bax and Bcl-2 in rat hippocampus following transient ischemia
Masoomeh Majidy-Zolbin, Leila Rezaei, Abolfazl Azami-Tameh, Zahra Rezvani, Mohammad Ali Atlasi, Hosein Nikzad, Tahereh Naji, Homayoon Naderian *
Kashan University of Medical Sciences , naderian_h@kaums.ac.ir
Abstract:   (6584 Views)

Background: Cerebral ischemia is considered as one of the leading causes of death and involved in neuronal apoptosis. This study aimed to evaluate the Bax and Bcl-2 expression in the rat hippocampus after reperfusion following transient ischemia.

Materials and Methods: In this experimental study, transient global cerebral ischemia (15 and 30 minutes) was induced in adult male Wistar rats (n=5 in each group) by the occlusion of bilateral common carotid artery followed by reperfusion for 24 and 72 hours. Five Sham-operated animals, without any intervention, considered as a control group. The expression of Bcl-2 and Bax genes were detected by reverse transcription (RT)-PCR in all groups.

Results: Transcription and expression of both Bcl-2 and Bax genes were increased after global cerebral ischemia in all experimental groups the two gene expression increment in the experimental groups (2, 3 and 4) was more detectable than the first group.

Conclusion: According to the results, transient cerebral ischemia activates Bcl-2 and Bax apoptotic genes that play an important role in the protection and induction of cell death in the hippocampal neurons, respectively.

Keywords: Transient cerebral ischemia, Gene expression, Apoptosis, Bcl-2 and Bax genes
Full-Text [PDF 451 kb]   (4024 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2012/10/16 | Accepted: 2013/03/9 | Published: 2013/03/9
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Majidy-Zolbin M, Rezaei L, Azami-Tameh A, Rezvani Z, Atlasi M A, Nikzad H, et al . Expression of the apoptotic genes Bax and Bcl-2 in rat hippocampus following transient ischemia. Feyz. 2012; 16 (5) :454-460
URL: http://feyz.kaums.ac.ir/article-1-1645-en.html

Volume 16, Issue 5 (Bimonthly 2012) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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