Feyz Medical Sciences Journal
مجله علوم پزشکی فيض
Feyz Med Sci J
Medical Sciences
http://feyz.kaums.ac.ir
29
journal29
3060-5806
3060-5814
fa
jalali
1397
9
1
gregorian
2018
12
1
22
6
online
1
fulltext
fa
ارزیابی اثر حفاظتی شش هفته تمرین مقاومتی و مصرف ویتامین D بر عوامل التهابی مغز در موش های صحرایی ماده مبتلا به آنسفالومیلیت خودایمن تجربی
Evaluating the protective effect of 6 weeks resistance training and vitamin D intake on brain neuro-inflammatory factors in female rats with experimental autoimmune encephalomyelitis
عمومى
General
پژوهشي
Research
<div style="text-align: justify;"><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">سابقه و هدف:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> مولتیپل اسکلروزیس</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">(</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">MS</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) یک بیماری خودایمن التهابی مزمن است که با کاهش توانایی بدنی همراه است. هدف از این پژوهش ارزیابی اثر حفاظتی 6 هفته تمرین مقاومتی و مصرف ویتامین </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">D</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> بر عوامل التهابی مغز در موش های صحرایی ماده مبتلا به آنسفالومیلیت خودایمن تجربی </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">(</span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">EAE</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">)</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> بود.</span></span></strong><br>
<strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">مواد و روش</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">­</span></span></span></strong><strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">ها:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در این پژوهش تجربی </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">48 سر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">موش</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ماده</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">نژاد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">لوئیز</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">4 هفته­ ای</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">­</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> طور</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تصادفی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">8 گروه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تقسیم</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">شدند. </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">پروتکل تمرین مقاومتی به ­مدت 6 هفته و 5 روز در هفته انجام شد. شروع</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">برنامه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تمرینی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بار</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">معادل</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">۵۰ درصد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">وزن</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بدن بود.</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">حیوانات</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">دو</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">جلسه</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">اول ۸ تا ۱۰ تکرار (بالا</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">رفتن</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">از</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">نردبان)</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">را</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">با</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">فواصل</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">استراحت</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">۲ دقیقه ­ای</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">انجام دادند. موش­ ها با نخاع خوکچه هندی و ادجوانت کامل ایمونیزه شدند. مدل </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">EAE</span></span></span><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> <strong>در انتهای هفته ششم تمرینات القاء شد</strong></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">. حیوانات </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ازای</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">هر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">کیلوگرم وزن</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بدن</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">5 میکروگرم</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">ویتامین </span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">D3</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> در 150 میکرولیتر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">روغن کنجد</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">هر</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">دو</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">روز</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">یک­بار به ­مدت 2 هفته</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به­ صورت</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">داخل صفاقی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">دریافت کردند.</span></span></strong><br>
<strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتایج:</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> شش هفته تمرین مقاومتی به همراه مصرف ویتامین </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">D</span></span></span><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;"> بر شاخص­ های </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">عامل رشد گرانولوسیت-مونوسیت</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">، عامل رونویسی هسته ­ای کاپا-بی</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">و </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">فاکتور تبدیل رشد بتا </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">تاثیر معنی­ داری نداشت (0/05</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">></span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">)؛ بااین­ وجود، مقادیر اینترلوکین-17 </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">در هر سه سطح تمرین (0/001</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">=</span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">)، مکمل (0/045</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">=</span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) و اثر تعاملی تمرین و مکمل (0/043</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">=</span></span></span></strong><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">P</span></span></span></em><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">) تفاوت معنی­ داری نشاد داد.</span></span></strong><br>
<strong><span style="font-family:b zar;"><span style="font-size:9.0pt;">نتیجه­ گیری:</span></span></strong> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">احتمالا اجرای تمرین مقاومتی و مصرف ویتامین </span></span></strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">D</span></span></span> <strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">به صورت پیش ­آماده سازی از طریق پیشگیری از افت مقادیر عوامل ضد­التهابی و نیز افزایش عوامل پیش­ران بیماری، اثر حفاظتی در برابر </span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:9.0pt;">بیماری دارد. </span></span></strong></div>
<div style="text-align: justify;"><span style="font-family:times new roman;"><strong>Background:</strong> Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease and is associated with reduced physical capacity. The aim of this study was to examine the protective effect of a 6-week resistance training program and vitamin D intake on the brain neuro-inflammatory factors in female Lewis rats with experimental autoimmune encephalomyelitis (EAE).<br>
<strong>Materials and Method</strong><strong>s:</strong> In this experimental study, four-week-old female Lewis rats (n=48) were randomly divided into 8 groups. Resistance training protocol was performed for 6 weeks and 5 days a week. A training program was started with a load equal to 50% of the body weight of the rat. Animals in the first two sessions performed 8 to 10 repetitions (climbing the ladder) at 2-minute breaks. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of the exercise. Rats received 5 μg of vitamin D3 in 150 μl of sesame oil per kilogram of their body weight via intraperitoneal injection once every two days for 2 weeks.<br>
<strong>Results:</strong> The results showed that six weeks of resistance training with vitamin D had no significant effect on granulocyte-monocyte growth factor, nuclear transcription factor kB and transforming growth factor beta (<em>P</em>>0.05). However, Interleukin-17 values showed a significant difference in all three levels of training (<em>P</em>=0.000), supplements (<em>P</em>=0.045), and interactive training and supplementation (<em>P</em>=0.043).<br>
<strong>Conclusion:</strong> The inadequacy of the training period (frequency and intensity of exercise) and the dose rate of vitamin D3 can be one of the possible causes of ineffectiveness of the present intervention.</span></div>
تمرین مقاومتی, عوامل نوروالتهابی مغز, ویتامین D, مولتیپل اسکلروزیس
Resistance training, Neuro-inflammatory factors, Vitamin D, Multiple sclerosis
573
580
http://feyz.kaums.ac.ir/browse.php?a_code=A-10-2573-1&slc_lang=fa&sid=1
Smatalsadat
Mousavi
عصمت السادات
موسوی
mousavi2753@gmail.com
2900319475328460023666
2900319475328460023666
No
Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, I. R. Iran.
گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران
Ziya
Fallahmohammadi
ضیاء
فلاح محمدی
zia-falm@umz.ac.ir
2900319475328460023667
2900319475328460023667
Yes
Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, I. R. Iran.
گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران
Akbar
Hajizadeh Moghaddam
اکبر
حاجی زاده مقدم
a.hajizadeh@umz.ac.ir
2900319475328460023668
2900319475328460023668
No
Department of Animal Physiology, Faculty of Sport Sciences, University of Mazandaran, I. R. Iran.
گروه فیزیولوژی جانوری، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، ایران