TY - JOUR JF - KAUMS JO - Feyz VL - 25 IS - 5 PY - 2021 Y1 - 2021/11/01 TI - Protective effect of ascorbic acid on sperm parameters and testis structure of the first-generation mouse following mancozeb exposure TT - اثر محافظتی آسکوربیک‌اسید بر پارامترهای اسپرم و ساختار بیضه نسل اوّل موش پس از مواجهه با منکوزب N2 - Background: This study aimed to investigate the possible protective effects of ascorbic acid on mancozeb-induced toxicity on sperm parameters and testicular structure in the first-generation mice pups. Materials and methods: In this experimental study, pregnant female NMRI mice were randomly divided into four groups including: control, sham, mancozeb (500 mg/ kg) and ascorbic acid+ mancozeb (100 mg/ kg ascorbic acid + 500 mg/ kg mancozeb). Treatments started at the first day of pregnancy and continued until weaning. Male pups from each group were maintain until puberty (8-10 weeks). Left testicular weight was measured and its structure was studied. Then, the left epididymis was isolated, and sperm parameters (number, viability, motility and morphology) were studied. Results: A significant decrease was observed in the sperm parameters of mancozeb group compared to the control and sham groups. However, concomitant use of ascorbic acid could improve the mancozeb-induced negative effects. Also, testicular parameters including weight, gonadosomatic index, diameter of seminiferous tubules, Johnsen`s score, as well as the number of germ cells including spermatogonia, spermatocytes, spermatids and Sertoli showed a significant decrease compared to the control and sham groups, also. While concomitant use of ascorbic acid could improve most of these damaged testicular and sperm parameters. Conclusion: This study was showed that consumption of ascorbic acid along during pregnancy and breastfeeding periods has a protective effect against the destructive effects of mancozeb on sperm parameters and testicular structure of first-generation male pups. SP - 1155 EP - 1167 AU - Saddein, Esmaeil AU - Haghpanah, Tahereh AU - Nematollahi-Mahani, Seyed Noreddin AU - Ezzatabadipour, Massood AU - Dehesh, Tania AD - Kerman University of Medical Sciences KW - Mancozeb KW - Ascorbic acid KW - Pregnancy KW - Breastfeeding period KW - Testis KW - Spermatozoa UR - http://feyz.kaums.ac.ir/article-1-4310-en.html DO - 10.48307/FMSJ.2021.25.5.1155 ER -