TY - JOUR JF - KAUMS JO - Feyz VL - 26 IS - 1 PY - 2022 Y1 - 2022/3/01 TI - Association of -308G/A TNFα gene polymorphism with reduced risk of idiopathic infertility in men TT - بررسی ارتباط پلی‌مورفیسم -308G/A ژن TNFα با کاهش خطر ناباروری ایدیوپاتیک در مردان N2 - Background: Infertility is one of the major health problems in the world and several factors play a role in the occurrence of this complication. One of the causes of idiopathic male infertility is a defect in the process of spermatogenesis due to genetic changes in cytokines involved in this process. Tumor Necrosis Factor-alpha (TNFα) as a multifunctional cytokine controls spermatogenesis-related cellular activity. In this study, the association of -308G/A polymorphism in the TNFα gene with male infertility was investigated. Materials and Methods: In a case-control study, blood samples were collected from 82 infertile men and 107 fertile men. After DNA extraction, the genotype of the samples at the -308G/A region was determined using the PCR-RFLP technique. Results: Data analysis showed a significant association between GA genotype and reduced risk of male infertility. Also, in the subgroup study, a significant association was observed between this genotype and the reduction of oligozoospermia and asthenozoospermia risk. Similar results were found for the association of carriers of allele A (GA + AA) and idiopathic male infertility. In addition, the allelic analysis showed a significant association between allele and a reduced risk of idiopathic male infertility. Subgroup analysis showed a significant association between this allele and reduced risk of asthenozoospermia, also. Conclusion: Based on findings of this research, the TNFα -308G/A polymorphism can be considered as a protective factor and a potential biomarker for idiopathic male infertility. SP - 75 EP - 81 AU - Zamani-Badi, Tayyebeh AU - Nikzad, Hossein AU - Rafatmanesh, Atieh AU - Rezazadeh Lavaf, Zeinab AU - Karimian, Mohammad AD - University of Mazandaran KW - Male infertility KW - -308G/A polymorphism KW - TNFα gene KW - Cytokine KW - PCR-RFLP UR - http://feyz.kaums.ac.ir/article-1-4464-en.html DO - 10.48307/FMSJ.2022.26.1.75 ER -