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Showing 5 results for Pcr-Rflp
Nader Pestehchian, Mahdi Baghaei, Hossein Ali Yosefi,
Volume 9, Issue 3 (10-2005)
Abstract
Background : Trichostrongyliases as zoonotic diseases are of major public health and socio-economic importance. Accurate identification of these parasites is the first step in strategic planning for the prevention,control and treatment of the parasitic disease. The aim of this study is using PCR-RFLP technique for genotyping. Materials and methods : samples were collected from elementary tract content of 70 sheep in Isfahan and Khorasgan slaughterhouses. Identification of Trichostrongylus was determined based on morphological character and key identification after direct and flotation methods . For genotyping studies, DNA was extracted and the ITS-2 of ribosomal DNA of each species with specific primers was amplified by PCR and restricted with the endonucleases DraI, RsaI, HinfI. The profile was visualized in agarose gel under ultraviolet transillumination. Results: The eggs of most species are morphologically indistinguishable at the generic level .rDNA-ITS2 fragment size of all species were the same size about 330bp, however, there were differences among species in their PCR-RFLP patterns. The two fragments produced with RsaI in all species were the same size about 138bp,190bp. The PCR product T.probulurus with DraI remained unrestricted. Restriction with DraI produced two fragments in the PCR product of T.axei and T.colubriformis about 110bp,215bp, however, the larger of the two fragments in T.vitrinus is smaller in size than in T.axei and T.colubriformis had a size about145bp,185bp.The two fragments produced with HinfI in T.colubriformis had a size about 90bp,238bp, By contrast other Trichostrongylus remained unrestricted. Conclusion: Based of our results from genotyping studies using PCR-RFLP technique, it can be concluded that four species had been identified .This study provides a pattern to distinguish Trichostrongylus species in Isfahan region using the DraI, RsaI, HinfI restriction endonucleases.
Seyeyd Mahdi Sajjadi, Shahram Samie, Maryam Kheirandish, Zahra Ataiei, Mahnaz Kavari, Maryam Abdollahi, Samiramis Tootian, Mohammad Reza Tabatabaiei, Reza Meshkani,
Volume 12, Issue 1 (4-2008)
Abstract
Background: Coagulation factor XIII is a transglutaminase with a tetrameric structure which consists of two identical pre enzyme subunits (FXIIIA) and two carrier protein subunits (FXIIIB). FXIII Val34Leu polymorphism has a high prevalence in Caucasians. The prevalence of FXIII Val34Leu polymorphism in Iranian patients was investigated in this study by PCR-RFLP method. Materials and Methods: In this descriptive study blood samples of 213 patients referred to the central laboratory of IBTO were assessed by DNA extraction and polymerase chain reaction (PCR). Then the genotypes of this polymorphism were identified by RFLP method in the presence of restricted enzyme. Data analyzed by SPSS 11.5 software and X2 Test. Results: The prevalence of Val34Leu polymorphism was 24.4% (22.06% heterozygote and 2.34% homozygote and 13.07% of Leu allele frequency). No relationship was seen between the polymorphism and sex. Conclusion : The high prevalence of FXIII Val34Leu polymorphism in Iranian thrombotic patients in comparison with reports from other Asian countries confirms the ethnic heterogenecity of this polymorphism which is not influenced by sex.
Somayeh Reisi, Zohreh Atai , Marzieh Abolhasani, Mahbobeh Kasiri, Mohamad Taghi Akbari, Soraya Heidari, Mostafa Montazer Zohouri , Efat Farrokhi, Abolfath Shirmardi, Golandam Banitalebi,
Volume 14, Issue 4 (1-2011)
Abstract
Background: Hearing loss is a sensorineural disorder occuring in 1 out of 500 births. It happens due to some genetic/environmental causes or both. More than 60% of cases are noninherited and 80% non syndromic with autosomal recessive inheritance. In the present study we investigated the frequency of mtDNA A1555G, A3243 and A7445G mutations among the patients in Fars province. Materials and Methods: Seventy two non syndromic hearing loss subjects were studied. DNA was extracted using standard phenol-chloroform method. The screening of the mitochondrial gene mutations were performed using PCR-RFLP procedure. Finally, the possible mutations were confirmed by direct sequencing. Results: None of the A1555G, A3243G and A7445G mutations was detected in this study. However, destroying a MTTL1 restriction site for the investigation of A3243G mutation, revealed a G3316A with allelic variant of 1.4% in the deaf subjects. Conclusion: Our data indicated that the mitochondrial A1555G, A3243 and A7445G mutations have no role in auditory deficits in patients studied.
Hassan Hassani-Bafrani, Monavareh Ahmadi, Gholam Reza Khosravi, Erfan Khosravi, Mohammad Karimian,
Volume 23, Issue 1 (4-2019)
Abstract
Background: Among probiotics, lactobacillus species with bacteriocin activities have been used in microbial biotechnology as safe biological preservatives. So, this study aimed to determine antimicrobial activity of bacteriocins of lactobacillus species isolated from lactic acid bacteria in traditional dairy products.
Materials and Methods: In this experimental study, isolated lactic acid bacteria from local yoghurt of Nadushan region were used as probiotics with a bacteriocin production potential. The bacteriocin effect of isolated lactic acid bacteria was investigated against seven pathogenic bacteria using well diffusion and disk methods.
Results: The results of well diffusion analysis showed that the most growth inhibitory effect against Staphylococcus epidermidis, Escherichia coli Staphylococcus aureus, Enterococcus faecalis and Candida albicans pathogen bacteria was caused by isolated lactic acid bacteria including Lactobacillus delbrucki, Lactobacillus caesium and Lactobacillus plantarum, respectively. Also, the results of disc method showed that the highest inhibitory effect against the bacterial pathogens was caused by Lactobacillus delbrucki, Lactobacillus plantarum and Lactobacillus caesium, respectively. Although antimicrobial compounds were inactivated by proteolytic enzymes, they showed stability against variant pH and temperature treatments.
Conclusion: It seems that bacteriocin of the probiotics isolated from dairy products of this region can be used as a safe and healthy biological preservative in food industry.
Tayyebeh Zamani-Badi, Hossein Nikzad, Atieh Rafatmanesh, Zeinab Rezazadeh Lavaf, Mohammad Karimian,
Volume 26, Issue 1 (3-2022)
Abstract
Background: Infertility is one of the major health problems in the world and several factors play a role in the occurrence of this complication. One of the causes of idiopathic male infertility is a defect in the process of spermatogenesis due to genetic changes in cytokines involved in this process. Tumor Necrosis Factor-alpha (TNFα) as a multifunctional cytokine controls spermatogenesis-related cellular activity. In this study, the association of -308G/A polymorphism in the TNFα gene with male infertility was investigated.
Materials and Methods: In a case-control study, blood samples were collected from 82 infertile men and 107 fertile men. After DNA extraction, the genotype of the samples at the -308G/A region was determined using the PCR-RFLP technique.
Results: Data analysis showed a significant association between GA genotype and reduced risk of male infertility. Also, in the subgroup study, a significant association was observed between this genotype and the reduction of oligozoospermia and asthenozoospermia risk. Similar results were found for the association of carriers of allele A (GA + AA) and idiopathic male infertility. In addition, the allelic analysis showed a significant association between allele and a reduced risk of idiopathic male infertility. Subgroup analysis showed a significant association between this allele and reduced risk of asthenozoospermia, also.
Conclusion: Based on findings of this research, the TNFα -308G/A polymorphism can be considered as a protective factor and a potential biomarker for idiopathic male infertility.
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