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Showing 3 results for Klebsiella Pneumonia
Marzieh Tavakol , Hasan Momtaz,
Volume 21, Issue 1 (4-2017)
Abstract
Background: Urinary tract infection (UTI) is the second prevalent infection in human mostly caused by Escherichia coli and Klebsiella pneumonia. The aim of this study was to determine the antibiotic resistance profile and detect the prevalence of antibiotic resistance encoding genes in K .pneumoniae isolated from UTI.
Materials and Methods: Fifty K. pneumonia strains isolated from 122 UTI samples of hospitalized patients in Payambaran Hospital (Tehran, Iran) which were subjected to this study (2014) were confirmed by standard biochemical tests. Isolates were tested for susceptibility to 10 antimicrobial drugs by using disk diffusion method. Antibiotic resistance encoding genes frequently include the aadA1, aac(3)-IV, sul1, blaSHV, Cat1, cmlA, tetA, tetB, dfrA1, CITM, qnr in isolates were determined by PCR.
Results: The highest antibiotic resistance in K. pneumoniae isolates were for Tetracycline and the lowest resistance (2%) for Gentamicin and Imipenem. To determine the frequency of antibiotic resistant genes, 64% and 4% of isolates had tetA and Gentamicin-(aac(3)-IV) resistant genes, respectively.
Conclusion: Frequency of antibiotic resistance encoding genes may have important and basic role in the occurrence and transfer of antibiotic resistance which can be due to the indiscriminate use of antibiotics.
Zahra Soroush, Maryam Ghane,
Volume 21, Issue 3 (8-2017)
Abstract
Background: Extended-spectrum beta-lactamases (ESBL) producing isolates of Klebsiella pneumoniae which have been increased in the hospitals were resulting in limitation of therapeutic options. The aims of this study were to evaluate the antibiotic susceptibility pattern and presence of ESBL genes in clinical isolates of Klebsiella pneumoniae from patients admitted to the intensive care units (ICUs).
Materials and Methods: In this descriptive cross-sectional study, a total of 65 Klebsiella pneumoniae strains were isolated from ICUs of educational hospitals in Tehran. Identification was performed using biochemical tests and the antimicrobial susceptibility was performed as recommended by the Clinical and Laboratory Standards Institute (CLSI). Molecular analysis of the ESBL genes was performed by Multiplex PCR (M-PCR).
Results: Most of the isolates were resistant to Cotrimoxazole (72.3%), Gentamicin (67.7%) and Ampicillin (69.2%) and the highest susceptibility was seen for Ciprofloxacin (50.8%) Tetracycline (49.2%), Imipenem (46.3%) and Ceftriaxone (43.1%). Among the ESBL-producing genes, blaCTX-M (55.3 %) was the most prevalent, followed by blaTEM (41.5 %) and blaSHV (10.7 %). The results showed that 1.5 % of the isolates had concurrently blaTEM/ blaSHV and blaSHV/ blaCTX-M genes and 21.6% of isolates the blaTEM/ blaCTX-M genes.
Conclusion: These findings reveal the high prevalence of multi drug resistant and ESBL-producing Klebsiella pneumoniae in patients hospitalized in ICUs and emphasize the need for appropriate infection control policies.
Ali Shivaee, Maryam Meskini, Shahla Shahbazi, ِdonya Hassani, Faramarz Masjedian Jazi, Mohsen Zargar,
Volume 23, Issue 2 (5-2019)
Abstract
Background: Klebsiella pneumoniae is an opportunistic microorganism causing nosocomial infection all over the world .This study aimed to investigate the prevalence of biofilm formation in K. pneumoniae isolated from patients and its correlation with the virulence factors.
Materials and Methods: Biochemical tests were used for the identification of K. pneumonia isolated from patients referred to Motahari and Milad hospitals in Tehran, Iran, from October 2015 to June 2016. Kirby-bauer test was performed and biofilm formation was assessed phenotypically. Finally, virulence genes were detected by the PCR method.
Results: The highest resistance rate was against ceftazidime and cefotaxime (67%) and the least resistance rate was against imipenem and meropenem (39%). In addition, 81% of the isolates were biofilm producers according to the results of biofilm formation assay. Also, the results of PCR showed that all 57 biofilm producer isolates harbored fimA, mrkA, ecpA, and fimD virulence genes and 92% of these isolates harbored fimH virulence gene. Among non-biofilm producer isolates, 36% had fimA gene, 29% had ecpA gene, and none of these isolates carried mrkA and fimH genes.
Conclusion: It seems that antibacterial resistance has a significant association with biofilm formation in K. pneumoniae isolates. Therefore, understanding resistance pattern and mechanisms leading to biofilm formation can facilitate efficient treatment of infections caused by this bacterium.
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