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Showing 4 results for Hemati
Reza Ghanei , Masomeh Hemati-Maslak-Pak, Sercot Ghosi , Heidar Hossein-Pour, Edalat Amin-Pour, Vajiheh Baghi ,
Volume 15, Issue 3 (Quarterly 2011)
Abstract
Background: Restless legs syndrome is one of the most common sleep disorders that decreases the quality of life. Diabetic patients suffer from restless legs syndrome more than the others. The purpose of this study was to determine the relationship between restless legs syndrome and the quality of sleep in type II diabetes. Materials and Methods: In this cross-sectional study, type II diabetic patients (n=108) referred to the Saqqez diabetes unit were selected through purposive sampling. Data were collected using screening questionnaires for restless legs syndrome including Pittsburgh sleep quality index (PSQI) and Epworth sleepiness scale (ESS) and were analyzed using descriptive statistics, correlation coefficient and independent T tests. Results: The mean sleep quality scores in the presence and absence of restless legs syndrome were 8.1±0.6 and 5.5± 4.4, respectively and there was a significant difference in the quality of sleep between the two groups (P=0.001). Forty-two percent of samples suffered from restless legs syndrome and other sleep disorders. Conclusion: Considering the high incidence of sleep disorders in diabetic patients and also the lack of proper diagnosis of restless legs syndrome, using various screening methods for the diagnosis of restless legs syndrome is necessary for diabetic patients.
Neda Eivazi-Arvanagh , Arezo Chakerzehi , Mahdieh Hemati, Hananeh Zarinnehad , Ali Moradi ,
Volume 19, Issue 1 (Bimonthly 2015)
Abstract
Background: Cell migration plays a principal role in many biological and pathological processes, including embryonic and tumor metastasis. It is well-known that Rac1 from Rho GTPase family is the key regulator of the cell migration. Melittin is a natural peptide in bee venom with apoptotic and anti cancer effects. This study aimed to examine the effect of melittin on Rac1 expression.
Materials and Methods: HeLa cells were cultured in Dulbecco's modified Eagle's medium until they reached 80% confluency, then treated with melittin (0.5 and 1 μg/ml) with the consideration of IC50=1 μg/ml for 6 hours. In the control group the same volume of distilled water, as the melittin solvent, was added. Thereafter, cells were lyzed with buffer and after centrifugation the protein concentration of supernatant was measured using the Bradford method. The amount of Rac1 expression was determined using the Western blotting technique and chemiluminescence. Outcomes were normalized with β-Actin as an internal control and the results were displayed as the percentage against the control.
Results: The Rac1expression compared to the control (100%), in the presence of different concentrations of melittin (0.5 and 1 μg/ml) were 13.88%±1.15% and 1.7%±0.96%, respectively which showed a significant reduction in Rac1 melittin expression (P<0.05).
Conclusion: It can be concluded that melittin can dose-dependently inhibit the Rac1 expression in HeLa cell line.
Maghsoud Nabilpour, Abbas Sadegi, Ali Hematiafif, Mohaddese Faal Pakdeh,
Volume 25, Issue 4 (Bimonthly 2021)
Abstract
Background: One consequence of diabetes mellitus is increased inflammation, which is exacerbated by training. The objective of this study was to evaluate the effect of two months of chia supplementation in conjunction with continuous training on Interleukin-1 beta, Interleukin-13 responses in male Wistar diabetic rats.
Materials and Methods: 36 diabetic rats were randomly assigned to 3 equal groups (control group, diabetes + training group, diabetes + training+ chia group) and subjected to 8 weeks of supplementation and training. Interleukin-1 and interleukin-13 were measured in soleus muscle tissue to examine the inflammatory and anti-inflammatory indicators. ANOVA and Tukey's post hoc test was used for data analysis.
Results: There was a significant difference between the experimental groups of chia supplementation group and training group in the level of interleukin-1 and interleukin-13 after applying the diabetes (P <0.001). However, changes were not significant in the groups other than the control group (P=0/72)
Conclusion: Eight weeks of aerobic training has a positive effect on reducing inflammation in diabetic rats, but chia supplementation could not play a synergistic role in preventing inflammatory factors. However, chia supplementation could possibly affect and strengthen anti-inflammatory factors.
Sayyed Saeed Vaezi, Mohsen Salesi, Farhad Daryanoosh, Mohammad Hemati Nafar,
Volume 27, Issue 2 (Bimonthly 2023)
Abstract
Background: Factors such as exercises training and taking antioxidant supplements can moderate or increase apoptosis. Therefore, this present study aimed to the effect of endurance training and vitamin C consumption in the conditions of air pollution on the expression of apoptotic proteins BAX and Bcl-2 in the brain of male Wistar rats.
Materials and Methods: In this experimental study, 30 Wistar rats were randomly divided into five groups: 1) polluted air control, 2) supplement, 3) training, 4) training+supplement, and 5) healthy air control .Rats trained in polluted air were maintained in a special chamber to simulate polluted air and performed endurance training protocol .Vitamin C supplement groups received the supplement at a dose of 20 mg/kg. Data analysis was done through One-Way-ANOVA and Tukey's post-hoc tests in SPSS version 26 software.
Results: There is a significant difference in BAX and BCL-2 protein gene expression between the groups (P≤0.0001). This significant difference was the increase in BAX protein expression in the training+supplement group as well as the healthy air control group compared to other groups (P≤0.05). In the training and supplement groups were seen a decrease in BCL-2 protein expression compared to the control group (polluted and healthy) (P≤0.05).
Conclusion: Considering the increase in BAX and decrease in BCL-2 expression, it is possible that training in polluted air can lead to cell damage related to apoptosis. Also, the use of vitamin C supplement alone or together with training cannot modulate the apoptosis of brain cells.
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